FastQCFastQC Report
Fri 10 Feb 2017
ERR1630758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273328
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66050.2905432036204191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57310.25209736562431817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51530.22667208603421946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.16288894519400632No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG30870.13579210743016407No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT24330.10702371149257828No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG24260.10671579288162553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.020.8397981
CTAGTAC1306.9849193E-1018.4999983
CGAATTA1950.018.02564215
CTAGCGG2400.015.41666729
TACACGG855.367016E-415.2352945
AGTACCG1508.111601E-814.8000015
TCTAGCG2400.014.64583428
TACGTTA1904.5656634E-1014.60526419
TAGCACT6850.014.5839424
GTATCAA43350.014.1683982
ACGTTAA1854.9058144E-914.020
TCGCCAT5700.013.95614113
ATTACGT2009.858923E-1013.87500117
TCTTATA16250.013.66153837
TTAACGG1652.5981535E-713.45454535
GTCGCCA6000.013.25833412
ACGCTAA2102.044544E-913.21428534
CTAGCAC5900.013.1694923
CGAACGA2254.0745363E-1013.15555616
AACGCTA2254.0745363E-1013.15555633
Produced by FastQC (version 0.11.5)