FastQCFastQC Report
Fri 10 Feb 2017
ERR1630757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279586
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128221.0020428482337256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99020.7738440401817464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86510.6760780439923538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61490.4805460516135687No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA30040.23476343129731023No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC25340.19803280123414915No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC23360.18255904644158347No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT22140.17302471268050762No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT21930.1713835568691749No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20780.16239627504520993No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG20610.16106772034079772No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC20210.15794170927159254No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA18940.14801662412686603No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC18570.14512506388785124No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG18410.14387465946016914No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT18260.14270240530921718No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC17980.14051419756077355No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.137778937875219No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA17200.13441847597582343No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG16890.1319958173971894No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC16640.13004206047893616No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG16450.12855720522106368No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.1273068007933816No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16290.1273068007933816No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG16280.12722865051665147No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG16140.12613454664242965No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15940.12457154110782707No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA15400.12035142616440005No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC15380.1201951256109398No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.11667836315808396No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT14930.11667836315808396RNA PCR Primer, Index 33 (95% over 22bp)
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG14740.11519350790021148No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14540.11363050236560888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT14320.11191119627754602No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC14180.1108170924033242No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC13900.10862888465488056No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG13890.10855073437815042No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG13450.10511212220202472No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT13360.10440876971145356No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT13080.10222056196300992No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12890.10073570670513744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG200.001842014137.04
GCCGTCT2400.021.58333236
GTTACAC450.003826041720.5555553
CCGTCTT2850.020.12280837
GTACTAT2050.018.9512181
CTTGTAC902.1526048E-618.53
TATATAG609.237597E-418.55
TCTAGCG701.2193343E-418.528
GCAGTCG4350.018.2873579
CAGTCGG4350.017.8620710
GACGGAC1351.1514203E-917.8148167
TTGTATA650.001580066417.0769232
TCGGTGA4350.017.01149613
TTACACA1107.8093035E-716.8181824
AGCTGAT1554.0017767E-1016.7096797
GATATGC3550.016.67605634
CTTATTG4550.016.6703328
GTACTGG1005.8788355E-616.6500021
TACACAT6900.016.6231885
GGGTGTA904.44659E-516.4444457
Produced by FastQC (version 0.11.5)