FastQCFastQC Report
Fri 10 Feb 2017
ERR1630756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503805
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95161.8888260338821568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88011.7469060449975686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64801.286211927233751No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50671.0057462708786138No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT16190.32135449231349433No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.3175831919095682No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT15010.2979327319101637TruSeq Adapter, Index 5 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.2753049294866069No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.2187354234277151No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10630.2109943331249194No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC10600.21039886464008892No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9850.19551215251932794No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC9090.18042695090362343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8910.17685413999464078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8490.16851758120701463No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC8430.16732664423735374No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA8270.16415081231825807No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG8240.1635553438334276No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7950.1577991484800667No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT7830.15541727454074492No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC7720.15323389009636665No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7710.15303540060142318No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG7470.14827165272277965No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA7390.14668373676323182No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC7260.14410337332896658No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.13914113595537955No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC6730.1335834300969621No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC6460.12822421373348816No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC6350.12604082928910987No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG6290.12484989231944899No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6160.12226952888518376No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6130.12167406040035332No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT6110.12127708141046634No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.11988765494586198No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA6020.11949067595597503No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC5870.11651333353182283No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5860.11631484403687935No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG5760.11432994908744454No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC5600.11115411716834886No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG5540.10996318019868799No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.1097646907037445No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG5420.10758130625936621No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT5410.10738281676442275No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT5330.1057949008048749No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5320.10559641130993142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5200.10321453737060966No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC5050.10023719494645746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGTA250.005493449529.624
GTATGTA250.005493449529.61
GCCTATG250.005493449529.61
GTATACT250.005493449529.64
ATACGCC250.005493449529.66
AATACGC250.005493449529.65
ACTTAGA405.9336082E-527.7500021
TATACTT358.8624534E-426.428575
CCGTCTT2250.025.4888937
GCCGTCT2450.023.40816336
TGTTTCA400.001929689723.1250028
AGACGGT400.001929689723.12500210
GACGGTC400.001929689723.12500211
ACCTTAG502.6991838E-422.21
GACACAT1151.3460522E-1020.91304226
GTGACAC1151.3460522E-1020.91304224
GCAGCGT806.944156E-720.81251
ATTAGAG1251.8189894E-1120.7200013
ATCTAAC450.003822870820.55555520
CTGAGAT450.003822870820.55555534
Produced by FastQC (version 0.11.5)