##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503805
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136082412838302	31.0	31.0	34.0	28.0	34.0
2	31.251365111501475	31.0	31.0	34.0	28.0	34.0
3	31.23832038189379	31.0	31.0	34.0	28.0	34.0
4	35.02247496551245	37.0	35.0	37.0	32.0	37.0
5	34.90003275076666	35.0	35.0	37.0	32.0	37.0
6	34.989805579539706	36.0	35.0	37.0	32.0	37.0
7	34.940006550153335	36.0	35.0	37.0	32.0	37.0
8	34.98771548515795	36.0	35.0	37.0	32.0	37.0
9	36.440017467075556	38.0	35.0	39.0	32.0	39.0
10	36.22766943559512	38.0	35.0	39.0	32.0	39.0
11	36.49544367364357	38.0	35.0	39.0	32.0	39.0
12	36.41137940274511	38.0	35.0	39.0	32.0	39.0
13	36.51685275056818	38.0	35.0	39.0	32.0	39.0
14	37.40365220670696	39.0	36.0	40.0	32.0	41.0
15	37.344845724040056	39.0	36.0	40.0	32.0	41.0
16	37.229279185399115	39.0	36.0	40.0	32.0	41.0
17	37.331181707208145	39.0	36.0	40.0	32.0	41.0
18	37.33839283055944	39.0	36.0	40.0	32.0	41.0
19	37.40412659659987	39.0	36.0	40.0	32.0	41.0
20	37.41306457855718	39.0	36.0	40.0	32.0	41.0
21	37.335087980468636	39.0	36.0	40.0	32.0	41.0
22	37.239697898988695	39.0	36.0	40.0	32.0	41.0
23	37.144119252488565	39.0	36.0	40.0	32.0	41.0
24	37.03357052827979	39.0	36.0	40.0	31.0	41.0
25	36.92076299361856	39.0	36.0	40.0	31.0	41.0
26	36.54559402943599	38.0	35.0	40.0	30.0	41.0
27	36.26665872708687	38.0	35.0	40.0	30.0	41.0
28	36.052635444269114	38.0	35.0	40.0	30.0	41.0
29	35.836111193815064	38.0	35.0	40.0	29.0	41.0
30	35.58549041791963	38.0	34.0	40.0	28.0	41.0
31	35.4094957374381	38.0	34.0	40.0	28.0	41.0
32	35.146530899852124	37.0	34.0	40.0	27.0	41.0
33	34.93840473992914	37.0	33.0	40.0	26.0	41.0
34	34.73396849971715	37.0	33.0	40.0	25.0	41.0
35	34.46960034140193	37.0	33.0	40.0	24.0	41.0
36	34.27143041454531	37.0	33.0	40.0	24.0	41.0
37	33.82724665297089	36.0	33.0	40.0	22.0	41.0
38	33.48766685523169	36.0	33.0	40.0	21.0	41.0
39	33.165978900566685	36.0	32.0	39.0	18.0	41.0
40	32.78088943142684	35.0	31.0	39.0	16.0	40.0
41	32.404882841575606	35.0	31.0	39.0	15.0	40.0
42	31.976770774406763	35.0	31.0	39.0	11.0	40.0
43	31.03966018598466	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	2.0
13	5.0
14	5.0
15	8.0
16	11.0
17	44.0
18	85.0
19	181.0
20	379.0
21	724.0
22	1158.0
23	1840.0
24	2898.0
25	4266.0
26	5947.0
27	8212.0
28	10693.0
29	13698.0
30	16964.0
31	20254.0
32	24776.0
33	30491.0
34	37787.0
35	48893.0
36	67737.0
37	89372.0
38	87376.0
39	29994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86239715763043	21.41661952541162	13.559809847063844	20.161173469894106
2	19.79357092525878	23.264556723335417	31.876023461458303	25.0658488899475
3	23.68495747362571	26.460833060410277	29.012217028413772	20.841992437550243
4	14.725141671877015	17.60522424350691	31.362531138039518	36.30710294657655
5	12.333541747303025	41.61113923045623	31.141215351177536	14.91410367106321
6	31.51477258066117	32.41452546123996	17.117734043925726	18.95296791417314
7	26.217087960619683	34.273379581385655	21.156002818550828	18.353529639443835
8	31.381586129554094	29.59954744395153	19.370589811534224	19.648276614960153
9	28.24842945187126	12.501066881035323	22.646261946586478	36.60424172050694
10	21.282043647839938	28.990383183969985	28.360576016514326	21.366997151675747
11	31.45026349480454	24.074393862704817	19.56352160061929	24.91182104187136
12	21.921179821557942	30.811921279066308	26.324867756373994	20.942031143001756
13	29.738093111422074	19.948392731314694	29.190857573862907	21.122656583400325
14	22.45868937386489	23.461855281309237	28.873274381953333	25.206180962872537
15	27.332598922202045	26.257579817588155	26.665277240202062	19.74454402000774
16	23.10100137950199	25.07438393823007	29.570964956679667	22.25364972558827
17	21.811812109844087	28.505473347823067	28.131122160359663	21.551592381973183
18	20.06966981272516	23.30405613282917	32.653903792141804	23.97237026230387
19	20.42357658220939	26.967775230495928	33.22217921616498	19.386468971129702
20	21.371959389049337	23.444983674239044	34.70390329591807	20.47915364079356
21	22.607953474062384	25.281805460446005	33.06834985758379	19.04189120790782
22	23.50770635464118	25.60613729518365	31.29782356268794	19.58833278748722
23	21.064102182392	25.125395738430544	32.87045583112514	20.940046248052322
24	20.099641726461627	27.392741239169915	31.851609253580254	20.6560077807882
25	19.53096932344856	26.655352765454886	32.866089062236384	20.947588848860175
26	20.722303272099325	27.822669485217492	32.00345371721202	19.451573525471165
27	20.77450600926946	27.3506614662419	31.989757942060915	19.885074582427727
28	19.519853911731722	27.25717291412352	33.39585752424053	19.82711564990423
29	19.595478409305187	27.542997786842133	33.527257569893116	19.334266233959568
30	17.311658280485506	28.801024205793908	34.14197953573307	19.745337977987514
31	20.08951876221951	28.624765534284098	31.561814590962772	19.723901112533618
32	18.621490457617533	28.148192256924805	32.258909697204274	20.971407588253392
33	17.658419428151763	28.823652008217465	32.471293456793795	21.04663510683697
34	19.820565496571092	28.31274004823295	32.0786812357956	19.788013219400362
35	18.289616022072032	29.37366639870585	32.534810095175715	19.801907484046406
36	19.347763519615725	29.19224700032751	30.8927065035083	20.567282976548466
37	19.079802701442024	28.11147170036026	31.887932831154913	20.920792767042805
38	18.706046982463455	27.14562181796528	31.713460565099595	22.43487063447167
39	19.44105358223916	28.25517809469934	31.611833943688527	20.691934379372974
40	18.212205119044075	28.34092555651492	31.72715634025069	21.719712984190313
41	18.83486666468177	28.315320411667212	30.980637349768266	21.869175573882753
42	18.140748900864423	29.042387431645178	31.87642044044819	20.94044322704221
43	17.584779825527733	27.236530006649396	31.213068548347078	23.965621619475787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	433.0
1	317.0
2	201.0
3	495.5
4	790.0
5	790.0
6	1287.5
7	1785.0
8	2096.5
9	2408.0
10	3649.0
11	4890.0
12	4890.0
13	9090.5
14	13291.0
15	18527.5
16	23764.0
17	21328.0
18	18892.0
19	18892.0
20	20020.5
21	21149.0
22	15659.0
23	10169.0
24	8872.0
25	7575.0
26	7575.0
27	7966.5
28	8358.0
29	9082.0
30	9806.0
31	10894.0
32	11982.0
33	11982.0
34	13479.5
35	14977.0
36	15755.0
37	16533.0
38	19215.5
39	21898.0
40	21898.0
41	23254.5
42	24611.0
43	26542.0
44	28473.0
45	29174.0
46	29875.0
47	29875.0
48	32524.0
49	35173.0
50	33429.0
51	31685.0
52	31725.0
53	31765.0
54	31765.0
55	28084.0
56	24403.0
57	23820.0
58	23237.0
59	21757.0
60	20277.0
61	20277.0
62	18567.0
63	16857.0
64	15881.5
65	14906.0
66	13960.0
67	13014.0
68	13014.0
69	10363.0
70	7712.0
71	6882.5
72	6053.0
73	4515.0
74	2977.0
75	2977.0
76	2273.5
77	1570.0
78	1201.0
79	832.0
80	659.5
81	487.0
82	487.0
83	426.5
84	366.0
85	336.0
86	306.0
87	239.0
88	172.0
89	172.0
90	146.5
91	121.0
92	73.0
93	25.0
94	16.0
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	503805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.956907037560164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01825762073089	25.71240463638848
2	9.521645157055287	6.2760794857141216
3	3.544916226896603	3.504884236373096
4	1.9338465091958215	2.549343985139077
5	1.277550008738367	2.1052048436912267
6	0.8116581089042044	1.604984450486265
7	0.6340721010674033	1.4627938702992205
8	0.504883952945498	1.3311530801584541
9	0.35150347891713823	1.0426020730246002
>10	2.7890680902143923	18.12516240620983
>50	0.33898744801114766	7.874249360719543
>100	0.24516056507949072	15.727750762647222
>500	0.02179205022928806	4.863800339861729
>1k	0.004237343100139345	1.8617303023621679
>5k	0.0024213389143653403	5.957856166924967
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9516	1.8888260338821568	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8801	1.7469060449975686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6480	1.286211927233751	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5067	1.0057462708786138	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	1619	0.32135449231349433	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.3175831919095682	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	1501	0.2979327319101637	TruSeq Adapter, Index 5 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.2753049294866069	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1102	0.2187354234277151	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1063	0.2109943331249194	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1060	0.21039886464008892	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	985	0.19551215251932794	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	909	0.18042695090362343	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	891	0.17685413999464078	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	849	0.16851758120701463	No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	843	0.16732664423735374	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	827	0.16415081231825807	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	824	0.1635553438334276	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	795	0.1577991484800667	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	783	0.15541727454074492	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	772	0.15323389009636665	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	771	0.15303540060142318	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	747	0.14827165272277965	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	739	0.14668373676323182	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	726	0.14410337332896658	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.13914113595537955	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	673	0.1335834300969621	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	646	0.12822421373348816	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	635	0.12604082928910987	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	629	0.12484989231944899	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	616	0.12226952888518376	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	613	0.12167406040035332	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	611	0.12127708141046634	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.11988765494586198	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	602	0.11949067595597503	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	587	0.11651333353182283	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	586	0.11631484403687935	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	576	0.11432994908744454	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	560	0.11115411716834886	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	554	0.10996318019868799	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	553	0.1097646907037445	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	542	0.10758130625936621	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	541	0.10738281676442275	No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT	533	0.1057949008048749	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	532	0.10559641130993142	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	520	0.10321453737060966	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	505	0.10023719494645746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.001587915959547841	0.0
3	0.0	0.0	0.0	0.002183384444378281	0.0
4	0.0	0.0	0.0	0.0025803634342652414	0.0
5	0.0	0.0	0.0	0.0027788529292087217	0.0
6	0.0	0.0	0.0	0.0027788529292087217	0.0
7	0.0	0.0	0.0	0.003374321414039162	0.0
8	0.0	0.0	0.0	0.003374321414039162	0.0
9	0.0	0.0	0.0	0.005359216363473963	0.0
10	0.0	0.0	0.0	0.007542600807852244	0.0
11	0.0	0.0	0.0	0.008932027272456605	0.0
12	0.0	0.0	0.0	0.010916922221891407	0.0
13	0.0	0.0	0.0	0.011512390706721847	0.0
14	0.0	0.0	0.0	0.013695775151100128	0.0
15	0.0	0.0	0.0	0.01746707555502625	0.0
16	0.0	0.0	0.0	0.026200613332539376	0.0
17	0.0	0.0	0.0	0.03830847252409166	0.0
18	1.9848949494348012E-4	0.0	0.0	0.042675241412848224	0.0
19	1.9848949494348012E-4	0.0	0.0	0.05220273717013527	0.0
20	1.9848949494348012E-4	0.0	0.0	0.057363464038665754	0.0
21	1.9848949494348012E-4	0.0	0.0	0.07225017615942676	0.0
22	1.9848949494348012E-4	0.0	0.0	0.0998402159565705	0.0
23	1.9848949494348012E-4	0.0	0.0	0.13398040908684908	0.0
24	1.9848949494348012E-4	0.0	0.0	0.19193934161034526	0.0
25	1.9848949494348012E-4	0.0	0.0	0.21000188565020197	0.0
26	1.9848949494348012E-4	0.0	0.0	0.2417602048411588	0.0
27	1.9848949494348012E-4	0.0	0.0	0.28622185170849834	0.0
28	1.9848949494348012E-4	0.0	0.0	0.35212036402973373	0.0
29	1.9848949494348012E-4	0.0	0.0	0.4305237145324084	0.0
30	1.9848949494348012E-4	0.0	0.0	0.579589325234962	0.0
31	1.9848949494348012E-4	0.0	0.0	0.9932414326971745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGGTA	25	0.0054934495	29.6	24
GTATGTA	25	0.0054934495	29.6	1
GCCTATG	25	0.0054934495	29.6	1
GTATACT	25	0.0054934495	29.6	4
ATACGCC	25	0.0054934495	29.6	6
AATACGC	25	0.0054934495	29.6	5
ACTTAGA	40	5.9336082E-5	27.750002	1
TATACTT	35	8.8624534E-4	26.42857	5
CCGTCTT	225	0.0	25.48889	37
GCCGTCT	245	0.0	23.408163	36
TGTTTCA	40	0.0019296897	23.125002	8
AGACGGT	40	0.0019296897	23.125002	10
GACGGTC	40	0.0019296897	23.125002	11
ACCTTAG	50	2.6991838E-4	22.2	1
GACACAT	115	1.3460522E-10	20.913042	26
GTGACAC	115	1.3460522E-10	20.913042	24
GCAGCGT	80	6.944156E-7	20.8125	1
ATTAGAG	125	1.8189894E-11	20.720001	3
ATCTAAC	45	0.0038228708	20.555555	20
CTGAGAT	45	0.0038228708	20.555555	34
>>END_MODULE