FastQCFastQC Report
Fri 10 Feb 2017
ERR1630755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221443
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.3994455738008241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44470.36407757054565787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33290.27254648804733417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.16726118206089027No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14620.11969449249780793No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12880.10544904674225486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTCG502.7022365E-422.210
TCTACGG555.143348E-420.1818182
GTCTACG851.2454384E-619.5882361
AAGACGG3450.019.3043485
GACGGAC3550.018.7605637
GTATTGA1002.8752947E-718.51
CAGTCGG1603.45608E-1117.3437510
CAAGACG4200.017.1785724
ACGGACC4050.016.9012348
CTAGCAC1452.9795046E-916.5862063
GCCGTCT1901.8189894E-1216.55263136
CGGTTGG904.446325E-516.44444533
GCGCAAG4100.016.2439021
CGCAAGA4100.016.2439022
AGTACCG803.3827825E-416.18755
GGTATCA23700.016.1582281
TCTTATA13550.016.110737
CGGACCA4250.016.1058839
AGACGGA4150.016.0481936
CTTATAC8350.015.95209637
Produced by FastQC (version 0.11.5)