FastQCFastQC Report
Fri 10 Feb 2017
ERR1630754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127144
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT24671.9403196375762914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19881.5635814509532497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18561.4597621594412635No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT18011.416504121311269TruSeq Adapter, Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13221.0397659346882275No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC12300.9674070345435097No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG12110.9524633486440571No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC11890.9351601333920594No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.5702195935317436No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC3760.2957276788523249RNA PCR Primer, Index 7 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3570.2807839929528723No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG3460.27213238532687345No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.19348140690870194No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.15494242748379788No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1900.14943685899452588No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1760.13842572201598188No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.12505505568489272No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1540.12112250676398414No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1460.11483042849053042No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1280.10067325237525955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCG200.001835784537.09
GTCCTAA250.005478197229.5999981
TACGGGC250.005478197229.5999984
TATTGTG250.005478197229.59999836
CCGTCTT2200.029.43181837
TATACAC3200.028.3281253
TTATACA3250.027.8923072
CGTCTTC556.188402E-726.9090937
GCCGTCT2500.025.936
GCTTATT809.586074E-1025.437527
GCCCCGT451.3141884E-424.66666614
TGACAGC451.3141884E-424.6666669
GTGACAG451.3141884E-424.6666668
CTTATAC3700.024.51
TCCGCTT851.9354047E-923.94117724
CCGCTTA851.9354047E-923.94117725
TACACAT3900.023.7179495
GGGCAGT2850.023.368427
CTTATTG2300.023.32608828
GCAGTCG2700.023.2962979
Produced by FastQC (version 0.11.5)