##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127144
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847535078336374	33.0	31.0	34.0	30.0	34.0
2	31.985127099981124	33.0	31.0	34.0	30.0	34.0
3	31.9851978858617	33.0	31.0	34.0	30.0	34.0
4	35.66284685081482	37.0	35.0	37.0	33.0	37.0
5	35.68556125338199	37.0	35.0	37.0	33.0	37.0
6	35.74027873906751	37.0	35.0	37.0	35.0	37.0
7	35.69950607185553	37.0	35.0	37.0	33.0	37.0
8	35.72546089473353	37.0	35.0	37.0	33.0	37.0
9	37.387969546341154	39.0	37.0	39.0	34.0	39.0
10	37.29048952368967	39.0	37.0	39.0	34.0	39.0
11	37.414703013905495	39.0	37.0	39.0	34.0	39.0
12	37.36333605990058	39.0	37.0	39.0	34.0	39.0
13	37.41850185616309	39.0	37.0	39.0	34.0	39.0
14	38.5177357956333	40.0	38.0	41.0	34.0	41.0
15	38.45988013590889	40.0	38.0	41.0	34.0	41.0
16	38.33646102057509	40.0	38.0	41.0	34.0	41.0
17	38.384123513496505	40.0	38.0	41.0	34.0	41.0
18	38.483396778455926	40.0	38.0	41.0	34.0	41.0
19	38.52302900648084	40.0	38.0	41.0	34.0	41.0
20	38.548842257597684	40.0	38.0	41.0	34.0	41.0
21	38.49792361416976	40.0	38.0	41.0	34.0	41.0
22	38.37192474674385	40.0	38.0	41.0	34.0	41.0
23	38.37396180708488	40.0	38.0	41.0	34.0	41.0
24	38.2561505065123	40.0	38.0	41.0	34.0	41.0
25	38.161556974768764	40.0	37.0	41.0	33.0	41.0
26	37.90982665324356	40.0	37.0	41.0	33.0	41.0
27	37.67496696658907	40.0	37.0	41.0	32.0	41.0
28	37.58339363241679	40.0	36.0	41.0	32.0	41.0
29	37.3843280060404	39.0	36.0	41.0	32.0	41.0
30	37.23555181526458	39.0	36.0	41.0	32.0	41.0
31	37.04221198011703	39.0	35.0	41.0	31.0	41.0
32	36.76695715094696	39.0	35.0	41.0	31.0	41.0
33	36.62878311206192	39.0	35.0	41.0	31.0	41.0
34	36.45366670861385	39.0	35.0	41.0	30.0	41.0
35	36.29691530862644	39.0	35.0	40.0	30.0	41.0
36	36.145669477128294	38.0	35.0	40.0	30.0	41.0
37	35.81683760145976	38.0	35.0	40.0	29.0	41.0
38	35.56370729251872	38.0	35.0	40.0	27.0	41.0
39	35.34945416220978	38.0	35.0	40.0	26.0	41.0
40	35.03687944378028	38.0	34.0	40.0	25.0	41.0
41	34.76930881520166	38.0	34.0	40.0	24.0	41.0
42	34.46345875542692	37.0	33.0	40.0	23.0	41.0
43	33.41889511105518	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	0.0
15	2.0
16	1.0
17	2.0
18	11.0
19	16.0
20	37.0
21	64.0
22	105.0
23	208.0
24	365.0
25	515.0
26	811.0
27	1116.0
28	1567.0
29	2184.0
30	2728.0
31	3342.0
32	4116.0
33	5250.0
34	6695.0
35	8865.0
36	12064.0
37	19632.0
38	27151.0
39	30293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.215566601648526	18.41140753790977	14.505599949663372	23.86742591077833
2	18.90140313345498	20.90543006354999	34.09441263449317	26.09875416850186
3	21.438683697225194	24.28899515509973	31.970836217202542	22.301484930472533
4	13.378531428930977	18.280060403951424	29.46422953501542	38.877178632102186
5	13.542125464040772	38.02696155540175	31.819039828855473	16.61187315170201
6	30.9617441640974	30.56770276222236	17.876580884666204	20.593972189014032
7	26.428301768073997	32.8761089787957	18.219499150569433	22.476090102560875
8	33.05464669980495	28.57704649845844	18.973762033599698	19.394544768136914
9	27.63716730636129	11.845623859560812	18.321745422513054	42.19546341156484
10	21.329358837223936	26.685490467501417	24.74438432014094	27.240766375133706
11	32.107688919650165	22.15283458126219	18.648146982948468	27.09132951613918
12	21.47486314729755	29.538161454728495	23.78012332473416	25.206852073239794
13	34.0889070660039	17.04917259170704	25.77943119612408	23.082489146164978
14	24.722362046183854	21.99789215377839	24.13483923740011	29.14490656263764
15	31.275561567985903	22.41316931982634	21.150821116214686	25.16044799597307
16	27.177059082614992	22.604291197382494	25.209998112376518	25.008651607626
17	20.293525451456613	31.002642672874853	24.167872648335745	24.53595922733279
18	24.15056943308375	20.251840432894987	27.401214371106775	28.19637576291449
19	22.778109859686655	26.70515321210596	28.98131252752784	21.535424400679545
20	23.93585226200214	22.25822689234254	29.372994400050334	24.432926445604984
21	29.051311898320016	20.264424589441894	27.71109922607437	22.97316428616372
22	25.055055684892718	25.528534574970113	25.647297552381552	23.769112187755614
23	26.215157616560752	21.993173095073303	26.391335808217452	25.40033348014849
24	23.26181337695841	24.067985905744667	28.93884099918203	23.731359718114895
25	22.411596300257976	25.62606178820864	28.129522431259048	23.832819480274335
26	25.689769080727366	22.16699175737746	27.544359151827848	24.598880010067326
27	21.77530988485497	26.865601208079028	27.47435978103568	23.884729126030326
28	22.933838796954635	22.779682879255017	28.860976530548037	25.425501793242304
29	20.842509280815452	24.26933241049519	30.31916567042094	24.56899263826842
30	21.160259233624867	26.245044988359655	31.660951362234947	20.933744415780534
31	23.02664695148808	24.446297111936072	26.804253444912856	25.722802491662993
32	20.840149751462906	25.523815516265024	30.211413829988043	23.424620902284023
33	21.91766815579186	24.154501982004657	30.85163279431196	23.076197067891524
34	21.223180016359404	26.698074624048324	26.951330774554837	25.12741458503744
35	20.339143018939154	24.948090354244005	32.82813188196061	21.88463474485623
36	21.150821116214686	25.612691121877557	26.859309129805574	26.377178632102183
37	20.79453218398037	26.742119171962496	28.557383753853898	23.905964890203236
38	20.673409677216384	23.880010067325237	30.41511986409111	25.031460391367265
39	22.553168061410684	24.13483923740011	28.548732146227902	24.763260554961303
40	19.69420499591015	23.10608443969043	32.93667023217769	24.263040332221735
41	20.092965456490276	24.69798024287422	28.823224060907314	26.38583023972818
42	20.04813439879192	23.506417919838924	32.07308248914617	24.372365192222993
43	18.74646070597118	24.57764424589442	31.816680299502924	24.859214748631473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	14.0
2	24.0
3	82.5
4	141.0
5	141.0
6	230.0
7	319.0
8	365.5
9	412.0
10	551.5
11	691.0
12	691.0
13	1290.5
14	1890.0
15	2968.5
16	4047.0
17	3602.5
18	3158.0
19	3158.0
20	3529.0
21	3900.0
22	2719.0
23	1538.0
24	1453.0
25	1368.0
26	1368.0
27	1448.0
28	1528.0
29	1646.5
30	1765.0
31	1847.5
32	1930.0
33	1930.0
34	1981.0
35	2032.0
36	2190.0
37	2348.0
38	2745.5
39	3143.0
40	3143.0
41	3673.0
42	4203.0
43	4926.5
44	5650.0
45	6601.5
46	7553.0
47	7553.0
48	9091.0
49	10629.0
50	10208.5
51	9788.0
52	10408.5
53	11029.0
54	11029.0
55	8927.5
56	6826.0
57	8182.0
58	9538.0
59	8575.0
60	7612.0
61	7612.0
62	6415.5
63	5219.0
64	4923.5
65	4628.0
66	4414.5
67	4201.0
68	4201.0
69	3811.5
70	3422.0
71	3037.0
72	2652.0
73	2248.5
74	1845.0
75	1845.0
76	1438.0
77	1031.0
78	824.5
79	618.0
80	444.5
81	271.0
82	271.0
83	197.5
84	124.0
85	82.5
86	41.0
87	28.0
88	15.0
89	15.0
90	11.0
91	7.0
92	5.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	127144.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.35632039262568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29888164520062	49.443151072799346
2	10.005565272698362	11.877870760712263
3	2.948269465203795	5.2499528094129495
4	1.2415858377060476	2.9478386711130686
5	0.658557269306196	1.9544768136915622
6	0.37896856945990354	1.3496507896558234
7	0.2954894789844702	1.2277417731076574
8	0.1669581809508666	0.792801862455169
9	0.12588116817724068	0.6724658654753665
>10	0.7857635024116181	8.55643994211288
>50	0.05167753219907776	2.063015163908639
>100	0.03047649334817406	3.0194110614736047
>500	0.001325064928181481	0.5702195935317436
>1k	0.010600519425451848	10.274963820549928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	2467	1.9403196375762914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1988	1.5635814509532497	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1856	1.4597621594412635	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	1801	1.416504121311269	TruSeq Adapter, Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1322	1.0397659346882275	No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	1230	0.9674070345435097	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	1211	0.9524633486440571	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	1189	0.9351601333920594	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.5702195935317436	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	376	0.2957276788523249	RNA PCR Primer, Index 7 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	357	0.2807839929528723	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	346	0.27213238532687345	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	246	0.19348140690870194	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	197	0.15494242748379788	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	190	0.14943685899452588	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	176	0.13842572201598188	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159	0.12505505568489272	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	154	0.12112250676398414	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	146	0.11483042849053042	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	128	0.10067325237525955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.005505568489272006	0.0
3	0.0	0.0	0.0	0.007865097841817152	0.0
4	0.0	0.0	0.0	0.008651607625998868	0.0
5	0.0	0.0	0.0	0.011797646762725728	0.0
6	0.0	0.0	0.0	0.011797646762725728	0.0
7	0.0	0.0	0.0	0.011797646762725728	0.0
8	0.0	0.0	0.0	0.011797646762725728	0.0
9	0.0	0.0	0.0	0.022808783741269743	0.0
10	0.0	0.0	0.0	0.029887371798905178	0.0
11	0.0	0.0	0.0	0.033819920719813754	0.0
12	0.0	0.0	0.0	0.03775246964072233	0.0
13	0.0	0.0	0.0	0.04011199899326748	0.0
14	0.0	0.0	0.0	0.04325803812999434	0.0
15	0.0	0.0	0.0	0.04719058705090291	0.0
16	0.0	0.0	0.0	0.05426917510853835	0.0
17	0.0	0.0	0.0	0.07157239036053609	0.0
18	0.0	0.0	0.0	0.07786446863398981	0.0
19	0.0	0.0	0.0	0.09280815453344239	0.0
20	0.0	0.0	0.0	0.09674070345435097	0.0
21	0.0	0.0	0.0	0.11247089913798528	0.0
22	0.0	0.0	0.0	0.14707732964198075	0.0
23	0.0	0.0	0.0	0.2013465047505191	0.0
24	0.0	0.0	0.0	0.28157050273705403	0.0
25	0.0	0.0	0.0	0.3004467375574152	0.0
26	0.0	0.0	0.0	0.3271880702195935	0.0
27	0.0	0.0	0.0	0.3610079909394073	0.0
28	0.0	0.0	0.0	0.41685018561630904	0.0
29	0.0	0.0	0.0	0.5041527716604794	0.0
30	0.0	0.0	0.0	0.6819039828855471	0.0
31	0.0	0.0	0.0	1.1585289120996665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATCCG	20	0.0018357845	37.0	9
GTCCTAA	25	0.0054781972	29.599998	1
TACGGGC	25	0.0054781972	29.599998	4
TATTGTG	25	0.0054781972	29.599998	36
CCGTCTT	220	0.0	29.431818	37
TATACAC	320	0.0	28.328125	3
TTATACA	325	0.0	27.892307	2
CGTCTTC	55	6.188402E-7	26.90909	37
GCCGTCT	250	0.0	25.9	36
GCTTATT	80	9.586074E-10	25.4375	27
GCCCCGT	45	1.3141884E-4	24.666666	14
TGACAGC	45	1.3141884E-4	24.666666	9
GTGACAG	45	1.3141884E-4	24.666666	8
CTTATAC	370	0.0	24.5	1
TCCGCTT	85	1.9354047E-9	23.941177	24
CCGCTTA	85	1.9354047E-9	23.941177	25
TACACAT	390	0.0	23.717949	5
GGGCAGT	285	0.0	23.36842	7
CTTATTG	230	0.0	23.326088	28
GCAGTCG	270	0.0	23.296297	9
>>END_MODULE