FastQCFastQC Report
Fri 10 Feb 2017
ERR1630753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272337
Sequences flagged as poor quality0
Sequence length43
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112664.136786408016539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90443.3208855205131877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82393.0252958650495527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54241.9916500512233006No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT21650.7949709367438137No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT20870.7663299514939211RNA PCR Primer, Index 39 (95% over 23bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.6539691632058809No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.5643742862703195No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.5320613798345433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT13340.4898342861968811No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13320.4890999019597044No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC11350.4167630545977961RNA PCR Primer, Index 39 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.39436433536390575No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC10410.3822469954504896No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10310.37857507426460596No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8290.3044022663097559No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG8150.2992615766495188No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.27980039436433535No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7530.27649566529704006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7330.26915182292527273No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7320.26878463080668435No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.2544641381817381No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.2342685716593779No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6360.23353418742220114No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5730.21040108395113405No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.19754935980054125No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5210.191307093784539No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC4930.18102571446406474RNA PCR Primer, Index 39 (95% over 24bp)
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4910.180291330226888No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4820.1769866011595927No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4710.17294748785512068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4560.16743960607629518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4400.16156453217888134No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA4170.15311911345134888No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA4100.15054876862123032No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA3800.1395330050635793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3630.13329073904757707No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3600.13218916269181197No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3600.13218916269181197No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3540.12998600998028179No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3450.1266812809129865No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA3440.12631408879439812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3400.12484532032004465No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.12484532032004465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3150.11566551735533548No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG3040.11162640405086345No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA2970.10905605922074488No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA2860.10501694591627285No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2850.10464975379768449No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2780.10207940896756593No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA2740.10061064049321246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTTC352.3816694E-531.7142853
AAATGCA352.3816694E-531.71428527
AATGCAC303.5923324E-430.83333228
CATGCGA250.005489071429.610
CCGTCTT3100.028.04838837
GCCGTCT3200.027.17187736
ACATATT358.851954E-426.4285733
TACACAA358.851954E-426.4285720
TGCACAT358.851954E-426.4285730
AGCGTCA659.3368726E-825.6153853
AAGTTCC451.3195926E-424.6666684
AAAATGC451.3195926E-424.66666826
AATGGGG400.001927424823.12500231
CCGTACA400.001927424823.12500217
ATGCACA400.001927424823.12500229
TACACTG400.001927424823.1250025
AAAACCC502.694771E-422.237
CGTCTTC705.078369E-621.14285737
ATGCCGT4150.020.95180734
TGCCGTC4150.020.95180735
Produced by FastQC (version 0.11.5)