FastQCFastQC Report
Fri 10 Feb 2017
ERR1630752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517513
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT136832.6439915519030444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136502.637614900495253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT109412.1141497894738874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79651.5390917716076697No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.451003163205562No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.37409688259038903No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.356512783253754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT13370.2583509979459453No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.2413465941918367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11500.22221663996846455No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10690.20656485924025098No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8960.17313574731456022No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.15419902495203017No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7950.15361932936950376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7710.14898176470929234No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.1460832867966602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6330.12231576791307658No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT6160.11903082627876015RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT6130.11845113069623371No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.10453843671559941No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.10202642252465155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1250.031.08000237
AACCGGG250.005493586429.59999814
GGGCGTC250.005493586429.5999989
GCCGTCT1350.028.77777936
AGTACCG405.9339145E-527.7500025
ATGCCGT1650.023.54545434
TGCCGTC1700.022.85294235
TATGCCG1750.022.233
ATAGTAC502.6993218E-422.1999993
AGCGTCA555.137844E-420.1818183
CGTATGC2100.018.50000231
GCAGCGT609.227464E-418.51
TCGTATG2150.018.06976730
AATCGTT1156.384653E-817.69565213
GTATGCC2200.017.6590932
AAATTCC1054.78467E-717.6190531
TCTGTCG852.7184338E-517.4117648
CAGCGTC650.00157834817.0769232
AGCTGAT1201.03791535E-716.9583347
CTCGTAT2300.016.89130429
Produced by FastQC (version 0.11.5)