FastQCFastQC Report
Fri 10 Feb 2017
ERR1630751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113926
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28052.462124536980145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24352.137352316415919No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT15791.3859873953267912TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15781.3851096325685093No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT15581.3675543774028756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12491.0963256850938328No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC10540.925161947228903No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC10240.8988290644804522No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG8670.7610203114302266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.28878394747467656No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.28615065919983146No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG2870.25191791162684546No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC2870.25191791162684546RNA PCR Primer, Index 1 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2830.24840686059371875No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.2317293681863666No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2500.2194406895704229No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2340.20539648543791583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2130.1869634675140003No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1870.16414163579867636No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.14307532959991573No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1590.13956427856678896No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.13780875305022558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1480.12990888822569038No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.12903112546740866No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.12113126064287344No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1340.11762020960974667No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1290.11323139581833823No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1260.11059810754349315No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.10357600547723962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1160.10182047996067622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGC250.00547583529.66
GCCGTCT2300.028.15217436
TATACAC2250.027.9555553
CGTCTTC405.8939848E-527.7537
CCGTCTT2350.027.55319237
TTATACA2300.027.3478262
ATGCCCC400.001920582723.12537
CTTATAC2850.022.0701751
TGCCGTC2900.021.68965735
ATGCCGT3000.020.96666734
TGGGCAG2100.020.2619046
TTCCTCC555.104104E-420.18181820
AGTCGGT1850.020.011
ATAGAGC759.146195E-619.73333437
CAGTCGG1950.018.9743610
GCAGTCG2050.018.9512189
GGCAGTC2150.018.9302358
TCTTATA2350.018.89361837
TACACAT3450.018.7681165
GGCCGCG500.006996987418.520
Produced by FastQC (version 0.11.5)