##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630751.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113926
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351263100609167	31.0	31.0	34.0	30.0	34.0
2	31.461036111159874	31.0	31.0	34.0	28.0	34.0
3	31.41022242508295	31.0	31.0	34.0	28.0	34.0
4	35.20375506907993	37.0	35.0	37.0	32.0	37.0
5	35.18779734213437	37.0	35.0	37.0	33.0	37.0
6	35.27425697382512	37.0	35.0	37.0	32.0	37.0
7	35.20663413092709	37.0	35.0	37.0	32.0	37.0
8	35.28714253111669	37.0	35.0	37.0	32.0	37.0
9	36.76901673015817	39.0	37.0	39.0	32.0	39.0
10	36.64617383213665	38.0	35.0	39.0	32.0	39.0
11	36.84391622632235	39.0	37.0	39.0	33.0	39.0
12	36.77042115057142	38.0	37.0	39.0	32.0	39.0
13	36.86742271298913	39.0	37.0	39.0	33.0	39.0
14	37.79877288766392	39.0	37.0	41.0	33.0	41.0
15	37.723750504713585	39.0	37.0	41.0	32.0	41.0
16	37.54848761476748	39.0	36.0	40.0	32.0	41.0
17	37.633595491810475	39.0	37.0	40.0	32.0	41.0
18	37.73460843003353	39.0	37.0	40.0	33.0	41.0
19	37.796780366202626	39.0	37.0	41.0	33.0	41.0
20	37.82041851728315	39.0	37.0	41.0	33.0	41.0
21	37.78830117795762	40.0	37.0	41.0	33.0	41.0
22	37.64983410283868	39.0	37.0	41.0	32.0	41.0
23	37.59729122412794	39.0	36.0	41.0	32.0	41.0
24	37.4640380597932	39.0	36.0	41.0	32.0	41.0
25	37.373900602145255	39.0	36.0	40.0	32.0	41.0
26	37.01344732545687	39.0	36.0	40.0	31.0	41.0
27	36.70373751382476	38.0	35.0	40.0	31.0	41.0
28	36.562215824307	38.0	35.0	40.0	30.0	41.0
29	36.28625599073082	38.0	35.0	40.0	30.0	41.0
30	36.06428734441655	38.0	35.0	40.0	30.0	41.0
31	35.819479311131786	38.0	35.0	40.0	30.0	41.0
32	35.504871583308464	38.0	35.0	40.0	29.0	41.0
33	35.29695591875428	37.0	34.0	40.0	28.0	41.0
34	35.04922493548444	37.0	34.0	40.0	27.0	41.0
35	34.79840422730545	37.0	33.0	40.0	25.0	41.0
36	34.565086108526586	37.0	33.0	40.0	24.0	41.0
37	34.083396239664346	37.0	33.0	40.0	22.0	41.0
38	33.739427347576495	36.0	33.0	40.0	21.0	41.0
39	33.38840124291207	36.0	33.0	40.0	19.0	41.0
40	32.95984235380861	35.0	32.0	40.0	16.0	41.0
41	32.52356793005986	35.0	31.0	39.0	14.0	41.0
42	32.10380422379439	35.0	31.0	39.0	10.0	41.0
43	31.085520425539386	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	10.0
18	11.0
19	30.0
20	74.0
21	136.0
22	205.0
23	361.0
24	580.0
25	878.0
26	1205.0
27	1669.0
28	2233.0
29	2835.0
30	3494.0
31	4318.0
32	5084.0
33	6269.0
34	7799.0
35	9961.0
36	13857.0
37	19746.0
38	22213.0
39	10952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.330530344258555	20.15255516738936	14.907044923897969	20.60986956445412
2	18.31803100258062	23.322156487544547	32.600108842582024	25.759703667292804
3	23.387110931657393	25.739515123852325	31.667924793286872	19.205449151203414
4	14.143391324192898	18.55151589628355	28.486912557274017	38.81818022224953
5	12.221968646314274	40.7562803925355	31.30628653687481	15.715464424275408
6	30.813861629478783	29.52969471411267	18.142478450924283	21.51396520548426
7	24.581746045678774	35.017467478889806	20.01825746537226	20.382529010059162
8	33.42871688639995	28.95914892122957	19.869915559222655	17.742218633147832
9	26.949072204764494	11.638256412056949	21.292768990397278	40.119902392781285
10	21.768516405385952	28.763407826132752	25.51568562049049	23.9523901479908
11	29.777223811948105	22.66910099538297	19.036918701613327	28.516756491055595
12	20.09111177430964	31.681091234661096	26.122219686463144	22.105577304566122
13	32.41138984955146	17.494689535312396	28.694942330986784	21.39897828414936
14	23.48893141161807	23.2668574337728	28.25167213805453	24.992539016554606
15	30.500500324772222	23.016695047662516	24.851219212471253	21.63158541509401
16	24.536980145006407	23.179958920702912	29.628004143040222	22.65505679125046
17	18.77797868792023	31.95144216421186	27.944455172655935	21.32612397521198
18	20.800344083001246	21.09878342081702	32.00586345522532	26.095009040956413
19	20.28334181837333	27.426575145269737	34.31525727226445	17.97482576409248
20	20.647613363060234	22.921018907009813	35.12894334919158	21.302424380738376
21	26.4838579428752	21.416533539314994	32.882748450748736	19.216860067061077
22	23.639028843284237	25.57712901357021	31.105278865228307	19.678563277917245
23	21.990590383231222	23.493320225409477	31.64685848708811	22.86923090427119
24	20.86793181538894	26.89377315099275	33.589347471165496	18.64894756245282
25	18.993030563699243	26.57690079525306	33.41116163123431	21.018907009813386
26	23.995400523146603	25.041693731018384	32.2946474027	18.668258343135015
27	19.57235398416516	28.221828204272946	32.60449765637344	19.601320155188457
28	19.974369327458174	25.049593595842918	33.84038762003406	21.135649456664854
29	18.714779769323947	27.33967663219985	34.63300739076243	19.31253620771378
30	17.4657233642891	28.06295314502396	36.23492442462651	18.236399066060425
31	20.345662974211333	27.68375963344627	31.5801485174587	20.390428874883696
32	18.986886224391274	28.390358653863036	34.37933395361902	18.243421168126677
33	17.73519653108158	26.834963046187877	34.82611519758439	20.603725225146146
34	18.344363885329074	30.766462440531573	30.912171058406336	19.97700261573302
35	17.535944384951634	27.881256254059654	35.90927444130401	18.67352491968471
36	19.435422993873217	29.02059231430929	30.143250882151573	21.400733809665923
37	17.908993557221354	29.320787177641627	32.104172884152874	20.66604638098415
38	17.944104067552622	27.07195899092393	33.062689816196475	21.921247125326968
39	19.768095079261975	27.689026209995966	31.38265189684532	21.16022681389674
40	17.469234415322227	26.928005898565736	34.628618576971014	20.97414110914102
41	17.663219984902483	28.83713989782842	30.52156663097098	22.978073486298122
42	17.34195881537138	27.477485385250073	33.678001509751944	21.502554289626598
43	16.514228534311744	28.434246791777117	32.76073942734757	22.29078524656356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	16.5
2	27.0
3	139.0
4	251.0
5	251.0
6	399.0
7	547.0
8	601.5
9	656.0
10	907.5
11	1159.0
12	1159.0
13	2177.5
14	3196.0
15	4596.0
16	5996.0
17	5245.0
18	4494.0
19	4494.0
20	5101.0
21	5708.0
22	4048.0
23	2388.0
24	2164.0
25	1940.0
26	1940.0
27	1980.5
28	2021.0
29	2137.0
30	2253.0
31	2293.0
32	2333.0
33	2333.0
34	2401.0
35	2469.0
36	2513.0
37	2557.0
38	2818.5
39	3080.0
40	3080.0
41	3485.5
42	3891.0
43	4396.5
44	4902.0
45	5609.5
46	6317.0
47	6317.0
48	7563.5
49	8810.0
50	9361.5
51	9913.0
52	7730.5
53	5548.0
54	5548.0
55	6568.0
56	7588.0
57	6860.0
58	6132.0
59	5069.0
60	4006.0
61	4006.0
62	3782.5
63	3559.0
64	3309.0
65	3059.0
66	2835.5
67	2612.0
68	2612.0
69	2462.0
70	2312.0
71	1954.0
72	1596.0
73	1373.0
74	1150.0
75	1150.0
76	935.0
77	720.0
78	555.5
79	391.0
80	287.5
81	184.0
82	184.0
83	140.0
84	96.0
85	65.0
86	34.0
87	25.5
88	17.0
89	17.0
90	10.5
91	4.0
92	3.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	113926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.50948861541703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.96093446620793	48.01098958973369
2	8.572671212662513	9.688745325913311
3	2.47596265863092	4.197461510103049
4	1.1727426645334658	2.6508435300107087
5	0.6290871246835148	1.7774695855204254
6	0.4224980195405334	1.4325088215157207
7	0.32619332390997063	1.2903112546740867
8	0.2019292005156961	0.9128732686129593
9	0.147563646530701	0.7504871583308463
>10	0.9630469563056276	9.844109334129172
>50	0.07921837866385001	3.2459666801256954
>100	0.0341726339334255	3.7787686744026825
>500	0.0015533015424284316	0.7610203114302266
>1k	0.012426412339427453	11.658444955497428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2805	2.462124536980145	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2435	2.137352316415919	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	1579	1.3859873953267912	TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1578	1.3851096325685093	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	1558	1.3675543774028756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	1.0963256850938328	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	1054	0.925161947228903	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	1024	0.8988290644804522	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	867	0.7610203114302266	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.28878394747467656	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.28615065919983146	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	287	0.25191791162684546	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	287	0.25191791162684546	RNA PCR Primer, Index 1 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	283	0.24840686059371875	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	264	0.2317293681863666	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	250	0.2194406895704229	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	234	0.20539648543791583	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	213	0.1869634675140003	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	187	0.16414163579867636	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.14307532959991573	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	159	0.13956427856678896	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	157	0.13780875305022558	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	148	0.12990888822569038	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	147	0.12903112546740866	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	138	0.12113126064287344	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	134	0.11762020960974667	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	129	0.11323139581833823	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	126	0.11059810754349315	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118	0.10357600547723962	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	116	0.10182047996067622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0017555255165633832
2	0.0	0.0	0.0	8.777627582816916E-4	0.0017555255165633832
3	0.0	0.0	0.0	8.777627582816916E-4	0.0017555255165633832
4	0.0	0.0	0.0	8.777627582816916E-4	0.0017555255165633832
5	0.0	0.0	0.0	8.777627582816916E-4	0.0026332882748450748
6	0.0	0.0	0.0	8.777627582816916E-4	0.0026332882748450748
7	0.0	0.0	0.0	8.777627582816916E-4	0.0026332882748450748
8	0.0	0.0	0.0	8.777627582816916E-4	0.0026332882748450748
9	0.0	0.0	0.0	0.0035110510331267664	0.0026332882748450748
10	0.0	0.0	0.0	0.0052665765496901495	0.0035110510331267664
11	0.0	0.0	0.0	0.0052665765496901495	0.0035110510331267664
12	0.0	0.0	0.0	0.007022102066253533	0.0035110510331267664
13	0.0	0.0	0.0	0.007022102066253533	0.0035110510331267664
14	0.0	0.0	0.0	0.010533153099380299	0.0035110510331267664
15	0.0	0.0	0.0	0.01667749240735214	0.0035110510331267664
16	0.0	0.0	0.0	0.029843933781577514	0.0035110510331267664
17	0.0	0.0	0.0	0.04827695170549304	0.0035110510331267664
18	0.0	0.0	0.0	0.05529905377174657	0.0035110510331267664
19	0.0	0.0	0.0	0.06407668135456349	0.0035110510331267664
20	0.0	0.0	0.0	0.0728543089373804	0.0035110510331267664
21	0.0	0.0	0.0	0.09743166616926777	0.0035110510331267664
22	0.0	0.0	0.0	0.1342977020170988	0.0035110510331267664
23	0.0	0.0	0.0	0.17906360268946508	0.0035110510331267664
24	0.0	0.0	0.0	0.27122869230904273	0.0035110510331267664
25	0.0	0.0	0.0	0.2914172357495216	0.0035110510331267664
26	0.0	0.0	0.0	0.3256499833225076	0.0035110510331267664
27	0.0	0.0	0.0	0.36163825641205694	0.0035110510331267664
28	8.777627582816916E-4	0.0	0.0	0.41693731018380353	0.0035110510331267664
29	8.777627582816916E-4	0.0	0.0	0.4906693818794656	0.0035110510331267664
30	8.777627582816916E-4	0.0	0.0	0.634622474237663	0.0035110510331267664
31	8.777627582816916E-4	0.0	0.0	1.0401488685638045	0.0035110510331267664
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCGC	25	0.005475835	29.6	6
GCCGTCT	230	0.0	28.152174	36
TATACAC	225	0.0	27.955555	3
CGTCTTC	40	5.8939848E-5	27.75	37
CCGTCTT	235	0.0	27.553192	37
TTATACA	230	0.0	27.347826	2
ATGCCCC	40	0.0019205827	23.125	37
CTTATAC	285	0.0	22.070175	1
TGCCGTC	290	0.0	21.689657	35
ATGCCGT	300	0.0	20.966667	34
TGGGCAG	210	0.0	20.261904	6
TTCCTCC	55	5.104104E-4	20.181818	20
AGTCGGT	185	0.0	20.0	11
ATAGAGC	75	9.146195E-6	19.733334	37
CAGTCGG	195	0.0	18.97436	10
GCAGTCG	205	0.0	18.951218	9
GGCAGTC	215	0.0	18.930235	8
TCTTATA	235	0.0	18.893618	37
TACACAT	345	0.0	18.768116	5
GGCCGCG	50	0.0069969874	18.5	20
>>END_MODULE