FastQCFastQC Report
Fri 10 Feb 2017
ERR1630750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902270
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67620.3554700436846504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.3184090586509801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52380.2753552334842057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34700.18241364264799423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31950.018.2394351
AAGACGG3750.016.285
ATCTCGC3200.016.187511
GACGGAC4000.015.7249997
CGCAAGA4500.015.2111112
CGCACAT750.004105720714.810
TCGTTTA2400.014.64583330
ACGCTTC2800.014.53571431
CCGGTCG2550.014.50980420
CGGCCTT3700.014.524
TCCTCGG3600.014.38888820
ACGGACC4250.014.3647068
GTAGAAC4150.014.265063
CCTATAC1956.730261E-1014.230773
CAAGACG4600.014.0760874
CGGACCA4550.013.8241779
ATGCGGC3350.013.80596935
TAACGCC2700.013.7037044
ATAACGC3250.013.6615383
GCGCAAG4750.013.6315781
Produced by FastQC (version 0.11.5)