##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630750.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1902270
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91245249097131	33.0	31.0	34.0	30.0	34.0
2	32.086127626467324	33.0	31.0	34.0	30.0	34.0
3	32.18975697456197	34.0	31.0	34.0	30.0	34.0
4	35.79016280549028	37.0	35.0	37.0	35.0	37.0
5	35.7634068770469	37.0	35.0	37.0	35.0	37.0
6	35.795713016553904	37.0	35.0	37.0	35.0	37.0
7	35.76861696814858	37.0	35.0	37.0	35.0	37.0
8	35.756179196433735	37.0	35.0	37.0	33.0	37.0
9	37.47135842966561	39.0	37.0	39.0	35.0	39.0
10	37.35683998591157	39.0	37.0	39.0	34.0	39.0
11	37.4740788636734	39.0	37.0	39.0	35.0	39.0
12	37.397299016438254	39.0	37.0	39.0	34.0	39.0
13	37.454129014282934	39.0	37.0	39.0	35.0	39.0
14	38.709220562801285	40.0	38.0	41.0	35.0	41.0
15	38.67961750960694	40.0	38.0	41.0	35.0	41.0
16	38.65808849427263	40.0	38.0	41.0	34.0	41.0
17	38.647578945155	40.0	38.0	41.0	34.0	41.0
18	38.6326436310303	40.0	38.0	41.0	34.0	41.0
19	38.66269719861008	40.0	38.0	41.0	34.0	41.0
20	38.643748784347125	40.0	38.0	41.0	34.0	41.0
21	38.56872473413343	40.0	38.0	41.0	34.0	41.0
22	38.51034395748238	40.0	38.0	41.0	34.0	41.0
23	38.49047716675341	40.0	38.0	41.0	34.0	41.0
24	38.4409447659901	40.0	38.0	41.0	34.0	41.0
25	38.395140542614875	40.0	38.0	41.0	34.0	41.0
26	38.20107398003438	40.0	38.0	41.0	33.0	41.0
27	38.08207562543698	40.0	38.0	41.0	33.0	41.0
28	37.98803061605345	40.0	37.0	41.0	33.0	41.0
29	37.882598684729295	40.0	37.0	41.0	33.0	41.0
30	37.80939351406477	40.0	37.0	41.0	33.0	41.0
31	37.76561844533111	40.0	37.0	41.0	33.0	41.0
32	37.66258785556204	40.0	37.0	41.0	33.0	41.0
33	37.62078306444406	40.0	37.0	41.0	32.0	41.0
34	37.548375362067425	40.0	37.0	41.0	32.0	41.0
35	37.497331083389845	40.0	37.0	41.0	32.0	41.0
36	37.45495171558191	40.0	37.0	41.0	32.0	41.0
37	37.27410146824583	40.0	36.0	41.0	31.0	41.0
38	37.15810426490456	39.0	36.0	41.0	31.0	41.0
39	37.09320496038943	39.0	36.0	41.0	31.0	41.0
40	36.988362850699424	39.0	36.0	41.0	31.0	41.0
41	36.86609208997671	39.0	35.0	41.0	31.0	41.0
42	36.77126117743538	39.0	35.0	41.0	30.0	41.0
43	35.92225551577852	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	8.0
11	3.0
12	2.0
13	5.0
14	6.0
15	3.0
16	10.0
17	41.0
18	72.0
19	139.0
20	295.0
21	577.0
22	982.0
23	1816.0
24	3012.0
25	4857.0
26	7270.0
27	10820.0
28	15904.0
29	21785.0
30	29203.0
31	38129.0
32	49611.0
33	63929.0
34	83777.0
35	112481.0
36	156944.0
37	241569.0
38	442523.0
39	616492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98748337512551	19.66014288192528	12.35287314629364	24.999500596655576
2	18.4727720039742	21.468403538929806	34.95686732167358	25.101957135422413
3	20.110394423504548	23.843092726058867	29.9088457474491	26.137667102987482
4	14.33539928611606	15.785929442192748	35.087395585274436	34.79127568641675
5	14.111876862905898	37.33202962776051	34.362051654076446	14.194041855257138
6	34.10751365473882	35.28305655874298	15.999253523422016	14.610176263096195
7	28.37883160644914	31.166763918896894	21.85599310297697	18.598411371676995
8	27.02224184789751	33.509701567075126	20.46465538540796	19.0034011996194
9	26.63643962213566	14.049898279424056	20.420497616006138	38.893164482434145
10	16.874523595493805	26.932086402035466	33.27387805096017	22.919511951510565
11	35.06431789388467	22.106746150651592	21.896418489488873	20.93251746597486
12	21.334773717716203	26.144343337170856	29.493552439979602	23.02733050513334
13	29.446713663149815	20.15008384719309	26.034316895077986	24.36888559457911
14	22.649886714293977	21.519973505338356	25.70555178812682	30.124587992240848
15	25.337728082764276	27.95160518748653	23.13567474648709	23.5749919832621
16	24.768145426253895	26.827053993386844	24.720150136415967	23.68465044394329
17	23.060711676050193	26.932927502405025	26.121370783327286	23.8849900382175
18	22.99815483606428	25.39429208261708	27.791060154447056	23.81649292687158
19	23.647431752590325	25.891172125933753	27.575423047201504	22.885973074274418
20	24.192885342248996	25.10758199414384	27.476015497274307	23.223517166332854
21	24.44868499214097	25.751812308452532	26.5910202021795	23.208482497227
22	24.253865119041986	25.53596492611459	26.839512792610932	23.37065716223249
23	23.47095838130234	25.673642542856694	27.163756985075725	23.691642090765246
24	23.78505680055933	26.09545437819027	26.818748127237455	23.30074069401294
25	23.926098818779668	25.599783416654837	26.923359985701296	23.5507577788642
26	23.610370767556656	26.326178723314776	26.9090612794188	23.154389229709768
27	24.04958286678547	25.65482292208782	26.48693403144664	23.808660179680068
28	23.177992608830504	25.893642858269335	27.39537499934289	23.532989533557277
29	22.921509564888265	26.20800412139181	27.5127085008963	23.357777812823628
30	22.867679141236522	26.828473350260477	27.296650843465965	23.007196665037036
31	23.529730269625237	26.502599525829666	26.60500349582341	23.362666708721687
32	22.884816561266277	26.21967438901944	26.829156744310744	24.066352305403544
33	22.741987204760626	25.944003742896644	27.430596077318153	23.883412975024576
34	23.48005277904819	25.798493378963027	27.196770174580898	23.524683667407885
35	23.54728823983977	25.840495828667855	27.493415761169548	23.118800170322825
36	22.81332302985381	26.428109574350643	27.094103360721668	23.664464035073884
37	23.57988087916016	25.377417506452815	27.288870665047547	23.753830949339473
38	22.917619475679057	25.496380639972244	27.906711455261345	23.679288429087354
39	23.09214780236244	25.43429691894421	27.719514054261484	23.754041224431862
40	22.598211610339224	25.433613524893943	28.428193684387598	23.539981180379232
41	22.099333953644855	25.563142981805946	28.711118821197836	23.626404243351367
42	22.696462647258276	25.424992246105973	28.419362130507235	23.45918297612852
43	22.089188180437056	24.923275875664338	28.550994338343138	24.43654160555547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	319.0
1	293.0
2	267.0
3	755.0
4	1243.0
5	1243.0
6	1721.0
7	2199.0
8	2119.0
9	2039.0
10	2984.5
11	3930.0
12	3930.0
13	6799.5
14	9669.0
15	13256.0
16	16843.0
17	17062.5
18	17282.0
19	17282.0
20	20729.0
21	24176.0
22	24329.0
23	24482.0
24	27646.5
25	30811.0
26	30811.0
27	34645.5
28	38480.0
29	45754.0
30	53028.0
31	58235.5
32	63443.0
33	63443.0
34	69599.5
35	75756.0
36	82689.0
37	89622.0
38	97782.0
39	105942.0
40	105942.0
41	109928.5
42	113915.0
43	113561.5
44	113208.0
45	116052.5
46	118897.0
47	118897.0
48	122294.5
49	125692.0
50	126886.0
51	128080.0
52	132069.0
53	136058.0
54	136058.0
55	128608.5
56	121159.0
57	115566.5
58	109974.0
59	102145.0
60	94316.0
61	94316.0
62	87271.5
63	80227.0
64	71256.0
65	62285.0
66	54522.5
67	46760.0
68	46760.0
69	40247.0
70	33734.0
71	29138.0
72	24542.0
73	19374.0
74	14206.0
75	14206.0
76	10938.0
77	7670.0
78	6184.5
79	4699.0
80	3838.5
81	2978.0
82	2978.0
83	2238.5
84	1499.0
85	1354.0
86	1209.0
87	1105.5
88	1002.0
89	1002.0
90	776.5
91	551.0
92	306.5
93	62.0
94	36.0
95	10.0
96	10.0
97	8.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1902270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.64169726401693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25573902365248	50.5818293510908
2	8.554032208114748	10.032459342698308
3	2.1923469898611727	3.8568884543135304
4	0.8534248801284635	2.001851338322932
5	0.4663627628086909	1.3674151975918893
6	0.2982371929504364	1.0493481109121818
7	0.20014299898733737	0.8215707609289519
8	0.1415593778714827	0.6641025745617654
9	0.10503776290358705	0.5543633424133565
>10	0.7125281950419073	8.285996853714625
>50	0.1092217823055788	4.540621275968084
>100	0.10445747974402568	12.24089052271172
>500	0.00592229625446453	2.4407892705942293
>1k	7.17854091450246E-4	0.6117101492003432
>5k	2.691952842938422E-4	0.9501634549772557
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6762	0.3554700436846504	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6057	0.3184090586509801	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5238	0.2753552334842057	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3470	0.18241364264799423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.6798141168183275E-4	0.0
3	0.0	0.0	0.0	6.308252771688561E-4	0.0
4	0.0	0.0	0.0	0.001471925646727331	0.0
5	0.0	0.0	0.0	0.0016822007391169497	0.0
6	0.0	0.0	0.0	0.001787338285311759	0.0
7	0.0	0.0	0.0	0.0019450446046039732	0.0
8	0.0	0.0	0.0	0.002102750923896187	0.0
9	0.0	0.0	0.0	0.003469539024428709	0.0
10	0.0	0.0	0.0	0.005204308536643064	0.0
11	0.0	0.0	0.0	0.0062031152254937525	0.0
12	0.0	0.0	0.0	0.006833940502662609	0.0
13	0.0	0.0	0.0	0.007464765779831464	0.0
14	0.0	0.0	0.0	0.00809559105700032	0.0
15	0.0	0.0	0.0	0.009041828972753605	0.0
16	0.0	0.0	0.0	0.010513754619480935	0.0
17	0.0	0.0	0.0	0.012616505543377123	0.0
18	0.0	0.0	0.0	0.01403586241700705	0.0
19	0.0	0.0	0.0	0.015665494383026595	0.0
20	0.0	0.0	0.0	0.01697971371046171	0.0
21	0.0	0.0	0.0	0.01966072113842935	0.0
22	0.0	0.0	0.0	0.02418163562480615	0.0
23	0.0	0.0	0.0	0.030857869808176546	0.0
24	0.0	0.0	0.0	0.03953171736924832	0.0
25	0.0	0.0	0.0	0.04273841252819	0.0
26	0.0	0.0	0.0	0.04862611511509933	0.0
27	0.0	0.0	0.0	0.05913986973458026	0.0
28	0.0	5.256877309740468E-5	0.0	0.0842677432751397	0.0
29	0.0	5.256877309740468E-5	0.0	0.12474569856014131	0.0
30	0.0	5.256877309740468E-5	0.0	0.18362272442923455	0.0
31	0.0	5.256877309740468E-5	0.0	0.38280580569530087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3195	0.0	18.239435	1
AAGACGG	375	0.0	16.28	5
ATCTCGC	320	0.0	16.1875	11
GACGGAC	400	0.0	15.724999	7
CGCAAGA	450	0.0	15.211111	2
CGCACAT	75	0.0041057207	14.8	10
TCGTTTA	240	0.0	14.645833	30
ACGCTTC	280	0.0	14.535714	31
CCGGTCG	255	0.0	14.509804	20
CGGCCTT	370	0.0	14.5	24
TCCTCGG	360	0.0	14.388888	20
ACGGACC	425	0.0	14.364706	8
GTAGAAC	415	0.0	14.26506	3
CCTATAC	195	6.730261E-10	14.23077	3
CAAGACG	460	0.0	14.076087	4
CGGACCA	455	0.0	13.824177	9
ATGCGGC	335	0.0	13.805969	35
TAACGCC	270	0.0	13.703704	4
ATAACGC	325	0.0	13.661538	3
GCGCAAG	475	0.0	13.631578	1
>>END_MODULE