FastQCFastQC Report
Fri 10 Feb 2017
ERR1630749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676799
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52240.31154598732465844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.2591246774359956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39520.2356871634584706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.16471860968428537No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC27060.1613789130360884No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT25830.15404350789808438No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA25570.15249293445427867No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA23720.14146000802719944No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG17960.10710884250288795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGT358.8710996E-426.42857211
GGTATCA21400.020.920561
CGAATTA1156.410119E-817.69565215
ACCGTCG2550.017.4117648
GTTAGAC752.0679794E-417.2666663
TACCGTC2500.017.027
CGTAGAC700.002593158715.8571433
CGTATGC1302.590823E-715.65384631
GCTTTAT8000.014.81
CGTCGTA2900.014.67241410
CGAGCCG3050.014.55737615
CCGTCGT2800.014.5357159
GTCTTAC1152.210929E-514.4782611
TAACGGC908.2768046E-414.38888936
GTACTAG908.2768046E-414.3888891
TTAACGG908.2768046E-414.38888935
CGCCTTA1551.2110286E-714.32258125
ATACCGT3100.014.3225816
GTATCAA32100.014.0046722
TTACACC2252.7284841E-1113.97777754
Produced by FastQC (version 0.11.5)