##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630749.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1676799
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.959145371627727	33.0	31.0	34.0	30.0	34.0
2	32.127971808189294	33.0	31.0	34.0	30.0	34.0
3	32.23838039025548	34.0	31.0	34.0	30.0	34.0
4	35.824586011799866	37.0	35.0	37.0	35.0	37.0
5	35.79551574160051	37.0	35.0	37.0	35.0	37.0
6	35.826679285949	37.0	35.0	37.0	35.0	37.0
7	35.80546445936573	37.0	35.0	37.0	35.0	37.0
8	35.79173293877203	37.0	35.0	37.0	35.0	37.0
9	37.50416716613023	39.0	37.0	39.0	35.0	39.0
10	37.40951718124832	39.0	37.0	39.0	34.0	39.0
11	37.511993387400636	39.0	37.0	39.0	35.0	39.0
12	37.44451779849582	39.0	37.0	39.0	35.0	39.0
13	37.50153357677336	39.0	37.0	39.0	35.0	39.0
14	38.76312664785702	40.0	38.0	41.0	35.0	41.0
15	38.74026940617212	40.0	38.0	41.0	35.0	41.0
16	38.717432441216864	40.0	38.0	41.0	35.0	41.0
17	38.71082103460224	40.0	38.0	41.0	35.0	41.0
18	38.69534690800746	40.0	38.0	41.0	35.0	41.0
19	38.706322582492	40.0	38.0	41.0	34.0	41.0
20	38.697389490332476	40.0	38.0	41.0	34.0	41.0
21	38.62308720365411	40.0	38.0	41.0	34.0	41.0
22	38.567369732448554	40.0	38.0	41.0	34.0	41.0
23	38.552496751250445	40.0	38.0	41.0	34.0	41.0
24	38.497610029586134	40.0	38.0	41.0	34.0	41.0
25	38.457261723080705	40.0	38.0	41.0	34.0	41.0
26	38.26766595161376	40.0	38.0	41.0	34.0	41.0
27	38.155600045085905	40.0	38.0	41.0	33.0	41.0
28	38.067482745397626	40.0	38.0	41.0	33.0	41.0
29	37.96926405609736	40.0	37.0	41.0	33.0	41.0
30	37.90411492373266	40.0	37.0	41.0	33.0	41.0
31	37.85411429753954	40.0	37.0	41.0	33.0	41.0
32	37.77568927462385	40.0	37.0	41.0	33.0	41.0
33	37.73649554895965	40.0	37.0	41.0	33.0	41.0
34	37.67455312175162	40.0	37.0	41.0	33.0	41.0
35	37.61644896019141	40.0	37.0	41.0	33.0	41.0
36	37.58110542766307	40.0	37.0	41.0	33.0	41.0
37	37.395801762763455	40.0	36.0	41.0	32.0	41.0
38	37.2904164422808	40.0	36.0	41.0	31.0	41.0
39	37.22484567321426	40.0	36.0	41.0	31.0	41.0
40	37.121843464839856	39.0	36.0	41.0	31.0	41.0
41	37.00732705589638	39.0	36.0	41.0	31.0	41.0
42	36.91968804847808	39.0	36.0	41.0	31.0	41.0
43	36.07347750088114	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	1.0
12	0.0
13	3.0
14	1.0
15	6.0
16	13.0
17	27.0
18	51.0
19	107.0
20	207.0
21	416.0
22	809.0
23	1456.0
24	2390.0
25	3870.0
26	5873.0
27	8924.0
28	12724.0
29	18104.0
30	24811.0
31	32332.0
32	42144.0
33	54599.0
34	72239.0
35	97166.0
36	136527.0
37	208543.0
38	390396.0
39	563055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88540308051233	19.24011166514293	11.92563926863029	24.948845985714446
2	19.35950582031597	20.915744820935604	33.20075930388794	26.52399005486048
3	21.28179942855405	23.050944090496237	28.745723250073503	26.92153323087621
4	14.855686340461796	14.880853340203567	34.230578620335535	36.032881698999105
5	14.763606132875795	36.62627422845553	33.974316540026564	14.635803098642114
6	35.69915058393999	34.06317632584466	15.21601575382619	15.021657336389154
7	29.482782372842543	30.110048968302106	21.215959694632453	19.191208964222902
8	28.55124555775618	31.465846532589776	20.015875486566966	19.967032423087083
9	26.892668709845367	13.785194289834383	20.079210448002414	39.24292655231784
10	17.929638555366505	26.367680324236836	32.35354982916856	23.349131291228108
11	35.720202600311666	21.715721443059067	21.244347116142126	21.319728840487144
12	22.904235987736158	25.46459056810029	27.94676046443253	23.68441297973102
13	30.616430472584966	19.637893390919245	24.944492452583763	24.801183683912026
14	23.16455341397508	21.051956734229922	25.0112863855477	30.772203466247294
15	26.16789489974648	27.671116216075987	22.16371789343863	23.997270990738905
16	25.470733224435367	26.101339516543128	24.099728112910373	24.32819914611113
17	24.339768809499528	25.886764006896474	25.16443533184359	24.609031851760406
18	23.885212240703865	24.50013388605313	27.099252802512407	24.5154010707306
19	25.005143729212627	24.840186569767752	26.845793681890317	23.308876019129304
20	25.605036739645005	24.318955342888444	25.909247321831657	24.166760595634898
21	25.465186942501756	24.58464013874054	26.04289482519968	23.907278093558023
22	25.345792787328712	24.76963547807459	26.300349654311578	23.584222080285112
23	24.77100713919796	24.513492672645917	25.93864857982382	24.776851608332304
24	24.265699108837733	25.264268406648622	26.446282470349757	24.02375001416389
25	24.760153125091318	25.280012690847265	25.74321668846415	24.216617495597266
26	24.458626227711253	25.77333359573807	25.972522645826963	23.795517530723718
27	24.937097410005613	24.458924414911984	25.991666264113945	24.612311910968458
28	24.08183688086646	25.14004361882372	26.213935003539486	24.564184496770334
29	24.15620476872899	25.576172218614158	26.575576440587096	23.692046572069756
30	24.387001662095457	25.463397819297363	25.898274032844725	24.251326485762455
31	23.635927740892022	26.227830527093587	26.1643166533377	23.97192507867669
32	23.74011434882774	25.24613862484412	25.99822638253005	25.015520643798094
33	23.115292888414174	25.72019663656765	26.587563566056517	24.57694690896166
34	24.987192859728566	24.558399665076138	26.11833618698484	24.336071288210455
35	24.08004775766207	24.74697325081897	26.63569098025464	24.53728801126432
36	23.285796329792657	25.807982948463113	26.556134635099376	24.35008608664485
37	24.14994283751362	24.448249313125782	27.111836302383292	24.289971546977306
38	24.03048904490043	24.75556104220005	26.706003522187217	24.507946390712306
39	23.887955562950598	24.508840952314497	26.980037559659802	24.6231659250751
40	23.116724186977688	24.352829408891587	27.55315335946646	24.97729304466427
41	22.220969835979147	24.74089023192404	28.148931386528737	24.889208545568074
42	23.090722263073868	24.506992191669962	28.310787399085996	24.091498146170174
43	23.120719895467495	24.171949052927634	27.99882394968031	24.70850710192456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	407.0
1	351.0
2	295.0
3	651.0
4	1007.0
5	1007.0
6	1369.0
7	1731.0
8	1579.0
9	1427.0
10	2156.5
11	2886.0
12	2886.0
13	5118.0
14	7350.0
15	9633.5
16	11917.0
17	12179.0
18	12441.0
19	12441.0
20	15731.0
21	19021.0
22	19280.0
23	19539.0
24	22511.5
25	25484.0
26	25484.0
27	28358.5
28	31233.0
29	35564.5
30	39896.0
31	44782.0
32	49668.0
33	49668.0
34	55614.5
35	61561.0
36	66996.0
37	72431.0
38	77316.5
39	82202.0
40	82202.0
41	84949.5
42	87697.0
43	89058.5
44	90420.0
45	92792.0
46	95164.0
47	95164.0
48	100534.5
49	105905.0
50	106849.0
51	107793.0
52	109662.0
53	111531.0
54	111531.0
55	108595.0
56	105659.0
57	104197.5
58	102736.0
59	103470.0
60	104204.0
61	104204.0
62	97063.5
63	89923.0
64	83088.0
65	76253.0
66	66179.5
67	56106.0
68	56106.0
69	49383.0
70	42660.0
71	36015.5
72	29371.0
73	21905.0
74	14439.0
75	14439.0
76	10354.5
77	6270.0
78	5106.0
79	3942.0
80	3230.5
81	2519.0
82	2519.0
83	1979.0
84	1439.0
85	1226.0
86	1013.0
87	911.5
88	810.0
89	810.0
90	595.5
91	381.0
92	216.5
93	52.0
94	31.5
95	11.0
96	11.0
97	8.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1676799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.78073354228142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49181051418215	50.553409904517345
2	7.92535347319012	9.158654745255859
3	1.8437152858482335	3.19593664978284
4	0.7299366813091032	1.6870510754183392
5	0.3963259239883875	1.145000130493575
6	0.28103999932234885	0.9743218389340554
7	0.1709685654341973	0.6915082386421622
8	0.12998585796177145	0.6008542578523166
9	0.10202447829457569	0.5305544275616236
>10	0.7179527582660605	8.25253163444544
>50	0.09647985469182896	3.8952021898765445
>100	0.10105074413959618	12.07033800109349
>500	0.01056045010785676	4.180463341951523
>1k	0.002691879439257606	2.7516602844799616
>5k	1.03533824586831E-4	0.3125132796948349
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5224	0.31154598732465844	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4345	0.2591246774359956	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3952	0.2356871634584706	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2762	0.16471860968428537	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2706	0.1613789130360884	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2583	0.15404350789808438	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2557	0.15249293445427867	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2372	0.14146000802719944	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1796	0.10710884250288795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.963744014637413E-5	0.0	4.1746208102461893E-4	0.0
3	0.0	1.1927488029274827E-4	0.0	0.0010138364824883602	0.0
4	0.0	1.1927488029274827E-4	0.0	0.001968035524830346	0.0
5	0.0	1.789123204391224E-4	0.0	0.002266222725562217	0.0
6	0.0	1.789123204391224E-4	0.0	0.002266222725562217	0.0
7	0.0	1.789123204391224E-4	0.0	0.0028625971270259585	0.0
8	0.0	1.789123204391224E-4	0.0	0.0031011468876114548	0.0
9	0.0	1.789123204391224E-4	0.0	0.005963744014637414	0.0
10	0.0	1.789123204391224E-4	0.0	0.009422715543127113	0.0
11	0.0	1.789123204391224E-4	0.0	0.01055582690590822	0.0
12	0.0	1.789123204391224E-4	0.0	0.011271476187664711	0.0
13	0.0	1.789123204391224E-4	0.0	0.011748575708835704	0.0
14	0.0	2.3854976058549653E-4	0.0	0.012523862430738568	0.0
15	0.0	2.3854976058549653E-4	0.0	0.013597336353373303	0.0
16	0.0	2.3854976058549653E-4	0.0	0.015923196519081893	0.0
17	0.0	2.3854976058549653E-4	0.0	0.018249056684790484	0.0
18	0.0	2.3854976058549653E-4	0.0	0.019441805487717966	0.0
19	0.0	2.3854976058549653E-4	0.0	0.02081346661108457	0.0
20	0.0	2.3854976058549653E-4	0.0	0.022602589815475797	0.0
21	0.0	2.3854976058549653E-4	0.0	0.02510736230162351	0.0
22	0.0	2.3854976058549653E-4	0.0	0.02946089543230882	0.0
23	0.0	2.3854976058549653E-4	0.0	0.034589715284896995	0.0
24	0.0	2.3854976058549653E-4	0.0	0.04174620810246189	0.0
25	0.0	2.3854976058549653E-4	0.0	0.04478771754992698	0.0
26	0.0	2.3854976058549653E-4	0.0	0.04949907532149053	0.0
27	0.0	2.3854976058549653E-4	0.0	0.061784387991643604	0.0
28	0.0	2.3854976058549653E-4	0.0	0.0910663711035133	0.0
29	0.0	2.3854976058549653E-4	0.0	0.13299149152641432	0.0
30	0.0	2.3854976058549653E-4	0.0	0.19292711887352032	0.0
31	0.0	2.3854976058549653E-4	0.0	0.4007635977836342	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGT	35	8.8710996E-4	26.428572	11
GGTATCA	2140	0.0	20.92056	1
CGAATTA	115	6.410119E-8	17.695652	15
ACCGTCG	255	0.0	17.411764	8
GTTAGAC	75	2.0679794E-4	17.266666	3
TACCGTC	250	0.0	17.02	7
CGTAGAC	70	0.0025931587	15.857143	3
CGTATGC	130	2.590823E-7	15.653846	31
GCTTTAT	800	0.0	14.8	1
CGTCGTA	290	0.0	14.672414	10
CGAGCCG	305	0.0	14.557376	15
CCGTCGT	280	0.0	14.535715	9
GTCTTAC	115	2.210929E-5	14.478261	1
TAACGGC	90	8.2768046E-4	14.388889	36
GTACTAG	90	8.2768046E-4	14.388889	1
TTAACGG	90	8.2768046E-4	14.388889	35
CGCCTTA	155	1.2110286E-7	14.322581	25
ATACCGT	310	0.0	14.322581	6
GTATCAA	3210	0.0	14.004672	2
TTACACC	225	2.7284841E-11	13.9777775	4
>>END_MODULE