FastQCFastQC Report
Fri 10 Feb 2017
ERR1630748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920165
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100771.0951296778295196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82890.9008167013524748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73530.799095814337646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47310.5141469193025163No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG21650.23528388930246208No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG15980.17366450582232534No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14810.16094939494547172No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13770.1496470741660463No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.14790825558459625No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG13320.144756646905718No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13310.14464797074437735No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12760.13867078187064275No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC12570.1366059348051708No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC12440.13519314470774263No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG12330.1339977069329957No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.1332369738036113No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.12845522270462362No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG11760.12780316573657985No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT11720.12736846109121736No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT11310.12291273847625155No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11310.12291273847625155No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG11250.12226068150820776No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11200.12171730070150463No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10920.11867436818396701No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA10220.11106703689012297No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10050.10921954214733226No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC10050.10921954214733226No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10040.10911086598599164No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG9880.10737204740454158No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT9690.10530720033906964No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT9360.10172088701482886No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT9360.10172088701482886No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9300.10106883004678509No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9300.10106883004678509No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9220.10019942075606006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTC200.001841744937.013
GCGTGTC251.2334346E-436.99999611
CCTAACT401.5996084E-632.3753
CCCTGCG303.598925E-430.8333347
CGTGTCT303.598925E-430.83333412
TGCGTGT303.598925E-430.83333410
GTTCCTA250.005495781529.59999827
ATTCCCG358.868046E-426.4285731
CAGTATT1250.023.6814
TAAGATA400.001930895623.1250024
AATACCC753.7390964E-722.1999995
GCACTAA855.1779352E-821.76470630
CCTGACA603.725246E-521.58333432
TGCACTA957.1395334E-921.4210535
TGTTTAC2050.020.75609614
GCCGTCT1251.8189894E-1120.7236
CTCGCTA450.003825236820.55555513
CCGTCTT1303.274181E-1119.92307737
CCGAGTT1303.274181E-1119.92307713
GTTTACC2150.019.79069715
Produced by FastQC (version 0.11.5)