FastQCFastQC Report
Fri 10 Feb 2017
ERR1630747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087763
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91660.4390345072692638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78960.3782038478505462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72040.3450583231908986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51030.24442429528639026No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG34900.16716456800891674No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT28050.13435433044842732No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG26570.12726540320908072No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC21060.10087351868962137No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA20980.10049033343344049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATA250.005497358729.614
ATACCCG603.7281614E-521.5833345
GCAGTCG2100.016.7380949
GGTATCA48700.015.7648881
TACGTTA1659.749783E-1015.6969719
CAGTCGG2250.015.62222210
CCAATAC1802.0190782E-1015.4166663
AATTTCG1101.4529187E-515.13636428
ACGTTAA1853.0559022E-1015.020
GTATAGT2350.014.9574471
ATCTCGC5250.014.80000111
TTCGGAA2151.2732926E-1114.62790731
AACGCTA1803.3342076E-914.38888833
ACGCTAA1803.3342076E-914.38888834
ATTACGT1854.9039954E-914.017
GCTTAGG10350.013.9420291
CGGTTGG1203.3028948E-513.87500133
GCGCAAG3000.013.5666671
TCGGTTG1501.3068438E-613.56666732
CTTAGGA11500.013.5130432
Produced by FastQC (version 0.11.5)