##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630747.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087763
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10326459468819	33.0	31.0	34.0	30.0	34.0
2	32.280170689872364	34.0	31.0	34.0	30.0	34.0
3	32.37630803879559	34.0	31.0	34.0	30.0	34.0
4	35.944894607290195	37.0	35.0	37.0	35.0	37.0
5	35.90127758754227	37.0	35.0	37.0	35.0	37.0
6	35.981814506723225	37.0	35.0	37.0	35.0	37.0
7	35.93210867325458	37.0	35.0	37.0	35.0	37.0
8	35.937210305959056	37.0	35.0	37.0	35.0	37.0
9	37.646941247641614	39.0	37.0	39.0	35.0	39.0
10	37.56935629187796	39.0	37.0	39.0	35.0	39.0
11	37.65869114454083	39.0	37.0	39.0	35.0	39.0
12	37.61439301300004	39.0	37.0	39.0	35.0	39.0
13	37.64306341284907	39.0	37.0	39.0	35.0	39.0
14	38.962047895283135	40.0	38.0	41.0	36.0	41.0
15	38.973357608119315	40.0	38.0	41.0	36.0	41.0
16	38.932928689702806	40.0	38.0	41.0	36.0	41.0
17	38.92838459154607	40.0	38.0	41.0	36.0	41.0
18	38.90834400264781	40.0	38.0	41.0	35.0	41.0
19	38.985042363524975	40.0	38.0	41.0	35.0	41.0
20	38.95383000848276	40.0	38.0	41.0	35.0	41.0
21	38.91297479646876	40.0	38.0	41.0	35.0	41.0
22	38.8543551159782	40.0	38.0	41.0	35.0	41.0
23	38.80328562197912	40.0	38.0	41.0	35.0	41.0
24	38.75437489791705	40.0	38.0	41.0	35.0	41.0
25	38.70376618418853	40.0	38.0	41.0	35.0	41.0
26	38.57102075283449	40.0	38.0	41.0	34.0	41.0
27	38.44575749258896	40.0	38.0	41.0	34.0	41.0
28	38.35929317647645	40.0	38.0	41.0	34.0	41.0
29	38.28859453874793	40.0	38.0	41.0	34.0	41.0
30	38.20710300929751	40.0	38.0	41.0	34.0	41.0
31	38.1546971567175	40.0	38.0	41.0	34.0	41.0
32	38.05030935024713	40.0	37.0	41.0	33.0	41.0
33	37.975019674167996	40.0	37.0	41.0	33.0	41.0
34	37.91011575547608	40.0	37.0	41.0	33.0	41.0
35	37.84847130636955	40.0	37.0	41.0	33.0	41.0
36	37.80551384424381	40.0	37.0	41.0	33.0	41.0
37	37.674099502673435	40.0	37.0	41.0	33.0	41.0
38	37.51334897687142	40.0	37.0	41.0	33.0	41.0
39	37.44396131170061	40.0	37.0	41.0	32.0	41.0
40	37.270226553492904	40.0	36.0	41.0	32.0	41.0
41	37.19276900682692	40.0	36.0	41.0	31.0	41.0
42	37.05436584516538	40.0	36.0	41.0	31.0	41.0
43	36.274622167362864	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	7.0
12	4.0
13	2.0
14	4.0
15	6.0
16	16.0
17	18.0
18	46.0
19	119.0
20	240.0
21	449.0
22	845.0
23	1491.0
24	2561.0
25	4181.0
26	6457.0
27	9761.0
28	14093.0
29	19845.0
30	27088.0
31	36021.0
32	46648.0
33	61016.0
34	80983.0
35	109768.0
36	154973.0
37	245998.0
38	456180.0
39	808935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96389820108892	19.327193747566174	12.154875816843196	24.55403223450171
2	18.30121522414182	21.64546454746061	35.1153363672026	24.937983861194972
3	20.717341958833448	24.153220456536495	29.613706153428332	25.515731431201722
4	14.697453686074521	15.511195475731679	34.11186997757888	35.67948086061492
5	13.859379632649874	37.99415929873266	34.091321668216175	14.05513940040129
6	34.5728418407645	34.17849631399733	16.018053773344963	15.230608071893217
7	28.093753936629778	31.13317938865666	21.8888350832925	18.88423159142106
8	26.917949978038695	33.7001374198125	20.502758215372147	18.87915438677666
9	26.64349353829913	14.177519191594065	20.254885252780127	38.924102017326675
10	16.947757001153864	27.69907312276346	32.92255873870741	22.430611137375266
11	34.308348217685634	22.375911442055443	21.937499610827473	21.378240729431454
12	21.1120706708568	26.46751570939805	29.166816348407366	23.253597271337792
13	29.269031015493617	20.540933046519168	26.35840370770054	23.831632230286676
14	22.716802625585373	21.71199508756502	25.845079158889206	29.726123127960406
15	25.424245951288533	27.939857158116126	23.60105050237982	23.03484638821552
16	24.705007225436987	26.93327738828593	24.87667422020603	23.485041166071053
17	23.144820556739436	27.135934490648605	26.234347481011973	23.484897471599986
18	22.357470651601737	25.547966890877937	28.160811356461434	23.933751101058885
19	23.560241272596556	26.146645955503573	28.041353352847043	22.25175941905283
20	23.98385257330454	25.00101783583673	28.34790155779176	22.667228033066973
21	23.75877913345528	26.079157452258713	27.019733561711746	23.14232985257426
22	23.83522459206337	25.584704777314283	27.233311443875575	23.34675918674677
23	23.09385691766738	26.22984505425185	27.43400472180032	23.242293306280455
24	23.877039683144112	26.318217153958567	27.136078185119672	22.66866497777765
25	23.70321727130905	25.69252352877218	27.436112240709313	23.16814695920945
26	23.577245118339583	26.271037469291294	27.172528682613876	22.979188729755247
27	23.713515375068912	25.80321616965144	26.887295157544223	23.595973297735423
28	22.892109880288135	26.047736261251874	27.72115417315088	23.33899968530911
29	22.720299191048028	26.687703537231002	27.602845725305023	22.989151546415947
30	22.506098632842907	27.338256305912118	27.43017286923851	22.725472192006468
31	23.18577348099377	26.626345998085032	27.269618246898712	22.91826227402248
32	22.439568092738497	26.643780927241263	27.44066256562646	23.47598841439378
33	22.491968676521235	26.45673862406796	27.643463362460203	23.407829336950602
34	23.00644278110111	26.480112924695	27.25740421685795	23.256040077345943
35	23.131552767244173	26.4119059490948	27.47744835021983	22.9790929334412
36	22.66061808739785	26.885810314676522	27.2462918444287	23.207279753496927
37	23.61719218129644	25.602379197255626	27.222630154859534	23.5577984665884
38	22.779692905756065	26.02436196062484	27.973337969874933	23.22260716374416
39	22.840954648587985	26.10669889254671	27.838935741269484	23.21341071759582
40	22.43889751854018	25.57512514590976	28.594098084887985	23.391879250662072
41	21.874130349086556	26.26145783788677	28.63984082484458	23.224570988182087
42	22.70458859554461	25.532256295374523	28.302206715992188	23.460948393088678
43	21.947031344074976	25.577711646388984	28.380184915625	24.095072093911043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	275.0
1	305.0
2	335.0
3	928.0
4	1521.0
5	1521.0
6	2231.0
7	2941.0
8	2967.5
9	2994.0
10	4414.0
11	5834.0
12	5834.0
13	9741.0
14	13648.0
15	18650.5
16	23653.0
17	23397.0
18	23141.0
19	23141.0
20	26362.5
21	29584.0
22	29039.0
23	28494.0
24	31132.5
25	33771.0
26	33771.0
27	37299.0
28	40827.0
29	50123.5
30	59420.0
31	63235.5
32	67051.0
33	67051.0
34	72816.0
35	78581.0
36	84515.0
37	90449.0
38	102824.0
39	115199.0
40	115199.0
41	122108.0
42	129017.0
43	124175.0
44	119333.0
45	126044.0
46	132755.0
47	132755.0
48	138667.0
49	144579.0
50	147437.0
51	150295.0
52	156744.0
53	163193.0
54	163193.0
55	145304.0
56	127415.0
57	120337.0
58	113259.0
59	103470.5
60	93682.0
61	93682.0
62	87729.0
63	81776.0
64	73627.0
65	65478.0
66	57390.0
67	49302.0
68	49302.0
69	42483.5
70	35665.0
71	31049.5
72	26434.0
73	21108.0
74	15782.0
75	15782.0
76	12406.0
77	9030.0
78	7367.0
79	5704.0
80	4474.5
81	3245.0
82	3245.0
83	2516.0
84	1787.0
85	1460.0
86	1133.0
87	957.5
88	782.0
89	782.0
90	561.5
91	341.0
92	192.0
93	43.0
94	26.0
95	9.0
96	9.0
97	7.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2087763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.253994455634135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.03027083837358	43.58141030111068
2	8.860649204565373	9.082873304082252
3	2.375700253645438	3.652923828857756
4	1.0334493133340243	2.1187362150320395
5	0.5687369960132271	1.457502142018798
6	0.34622613468098673	1.064728343240096
7	0.2575813688213642	0.9241451834611395
8	0.19410779271083844	0.7959039785117354
9	0.13672705923809758	0.6307027142512152
>10	0.9233979331323099	9.269158236400573
>50	0.12376376095858753	4.47973055000096
>100	0.1324166080267145	14.35025395817759
>500	0.013034311455463302	4.429145170666037
>1k	0.003563336944659032	2.75125588573172
>5k	3.750880994377929E-4	1.411530188457391
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9166	0.4390345072692638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7896	0.3782038478505462	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7204	0.3450583231908986	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5103	0.24442429528639026	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3490	0.16716456800891674	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2805	0.13435433044842732	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2657	0.12726540320908072	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2106	0.10087351868962137	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2098	0.10049033343344049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.579631404522448E-5	0.0
2	0.0	0.0	0.0	0.0011495557685426937	0.0
3	0.0	0.0	0.0	0.0017243336528140407	0.0
4	0.0	0.0	0.0	0.00320917652051502	0.0
5	0.0	0.0	0.0	0.0034486673056280813	0.0
6	0.0	0.0	0.0	0.0034486673056280813	0.0
7	0.0	0.0	0.0	0.003975547032876816	0.0
8	0.0	0.0	0.0	0.004406630446080326	0.0
9	0.0	0.0	0.0	0.0072326217104144485	0.0
10	0.0	4.789815702261224E-5	0.0	0.009962816660703346	0.0
11	0.0	4.789815702261224E-5	0.0	0.011064474272223427	0.0
12	0.0	4.789815702261224E-5	0.0	0.012022437412675672	0.0
13	0.0	4.789815702261224E-5	0.0	0.013076196867173142	0.0
14	0.0	4.789815702261224E-5	0.0	0.014369447106783673	0.0
15	0.0	4.789815702261224E-5	0.0	0.016381169701733388	0.0
16	0.0	4.789815702261224E-5	0.0	0.020213022263542366	0.0
17	0.0	4.789815702261224E-5	0.0	0.02476334718069053	0.0
18	0.0	4.789815702261224E-5	0.0	0.027206153188843753	0.0
19	0.0	4.789815702261224E-5	0.0	0.03017583892424571	0.0
20	0.0	4.789815702261224E-5	0.0	0.03261864493239894	0.0
21	0.0	4.789815702261224E-5	0.0	0.03745635879168277	0.0
22	0.0	4.789815702261224E-5	0.0	0.04593433258468514	0.0
23	0.0	9.579631404522448E-5	0.0	0.059297918393993954	0.0
24	0.0	9.579631404522448E-5	0.0	0.07745131990556399	0.0
25	0.0	9.579631404522448E-5	0.0	0.08291170980614179	0.0
26	0.0	9.579631404522448E-5	0.0	0.09330560988004864	0.0
27	0.0	9.579631404522448E-5	0.0	0.10733976988767403	0.0
28	0.0	9.579631404522448E-5	0.0	0.13119305208493492	0.0
29	0.0	9.579631404522448E-5	0.0	0.16658979012464536	0.0
30	0.0	9.579631404522448E-5	0.0	0.21894247575036055	0.0
31	0.0	9.579631404522448E-5	0.0	0.3929564802135108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	25	0.0054973587	29.6	14
ATACCCG	60	3.7281614E-5	21.583334	5
GCAGTCG	210	0.0	16.738094	9
GGTATCA	4870	0.0	15.764888	1
TACGTTA	165	9.749783E-10	15.69697	19
CAGTCGG	225	0.0	15.622222	10
CCAATAC	180	2.0190782E-10	15.416666	3
AATTTCG	110	1.4529187E-5	15.136364	28
ACGTTAA	185	3.0559022E-10	15.0	20
GTATAGT	235	0.0	14.957447	1
ATCTCGC	525	0.0	14.800001	11
TTCGGAA	215	1.2732926E-11	14.627907	31
AACGCTA	180	3.3342076E-9	14.388888	33
ACGCTAA	180	3.3342076E-9	14.388888	34
ATTACGT	185	4.9039954E-9	14.0	17
GCTTAGG	1035	0.0	13.942029	1
CGGTTGG	120	3.3028948E-5	13.875001	33
GCGCAAG	300	0.0	13.566667	1
TCGGTTG	150	1.3068438E-6	13.566667	32
CTTAGGA	1150	0.0	13.513043	2
>>END_MODULE