FastQCFastQC Report
Fri 10 Feb 2017
ERR1630746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294167
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66250.5119122957083592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64450.4980037352211886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52550.4060526964448947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.2993431295961031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGC250.00549659729.59999832
AACCGTC358.870003E-426.428577
GGTTTAG1103.8528015E-818.51
CCTATAC1054.7984213E-717.6190473
CAGTCGG2000.017.57499910
AGTCGGT1900.017.52631611
GACGGAC1705.456968E-1217.4117647
GTGCTAG2000.016.651
ACGGACC1708.54925E-1116.3235288
TCGGTGA2050.016.24390413
GTCGGTG2200.015.97727312
CGAGCCG1751.3096724E-1015.85714315
TAGTTCG957.060775E-515.5789487
CGGACCA1802.0190782E-1015.4166669
CGTATGC855.3648E-415.23529331
ATGCCGT855.3648E-415.23529334
GCGCAAG2201.8189894E-1215.1363641
CAAGACG2600.014.9423074
GTACTAA750.00410477814.81
CGGCTAA750.00410477814.826
Produced by FastQC (version 0.11.5)