FastQCFastQC Report
Fri 10 Feb 2017
ERR1630745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16227
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT7064.3507734023541005TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT6413.9502064460467126No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG3792.335613483700006No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC3532.1753867011770502No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC3282.0213224872126703No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC1520.9367104209034326RNA PCR Primer, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.8689221667591053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1210.745670795587601No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG1040.6409071300918222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT890.5484686017131941No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC850.5238183274788931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.28347815369445983No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT350.2156898995501325No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA330.20336476243298204No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA280.172551919640106No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT220.13557650828865472TruSeq Adapter, Index 12 (95% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA190.11708880261292907No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA190.11708880261292907No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA170.10476366549577863No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT170.10476366549577863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC307.817345E-637.037
TTATACA950.027.2631592
CCGTCTT1050.026.4285737
GCCGTCT1050.026.4285736
TATACAC1000.025.8999983
CTTATAC1000.025.8999981
TGCCGTC1403.6379788E-1219.82142835
ATGCCGT1455.456968E-1219.1379334
TATGCCG1601.8189894E-1218.533
TACACAT1451.1641532E-1017.8620685
ATACACA1451.1641532E-1017.8620684
GTATGCC1952.910383E-1115.17948732
CGTATGC1952.910383E-1115.17948731
ACACATC1802.5047484E-914.3888886
ACATCTC1957.741619E-913.2820528
CACATCT2001.1048542E-812.9499997
TCGTATG2451.0895747E-912.08163430
CTGTCTC1256.567583E-411.84000137
TCTCGTA2552.048182E-911.60784428
CTCGTAT2552.048182E-911.60784429
Produced by FastQC (version 0.11.5)