##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16227
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.686448511739695	31.0	31.0	34.0	30.0	34.0
2	31.887594749491587	33.0	31.0	34.0	30.0	34.0
3	31.947371664509767	33.0	31.0	34.0	30.0	34.0
4	35.609539656128675	37.0	35.0	37.0	33.0	37.0
5	35.614654588032295	37.0	35.0	37.0	33.0	37.0
6	35.665680655697294	37.0	35.0	37.0	33.0	37.0
7	35.629876132371976	37.0	35.0	37.0	33.0	37.0
8	35.634621310162075	37.0	35.0	37.0	33.0	37.0
9	37.24052505084119	39.0	37.0	39.0	34.0	39.0
10	37.15258519751032	39.0	37.0	39.0	33.0	39.0
11	37.26591483330252	39.0	37.0	39.0	34.0	39.0
12	37.219572317742035	39.0	37.0	39.0	34.0	39.0
13	37.28132125469896	39.0	37.0	39.0	34.0	39.0
14	38.40019720219387	40.0	38.0	41.0	34.0	41.0
15	38.348308374930674	40.0	38.0	41.0	34.0	41.0
16	38.24816663585382	40.0	38.0	41.0	33.0	41.0
17	38.23750539224749	40.0	38.0	41.0	33.0	41.0
18	38.378381709496516	40.0	38.0	41.0	34.0	41.0
19	38.40494237998398	40.0	38.0	41.0	34.0	41.0
20	38.415726874961486	40.0	38.0	41.0	34.0	41.0
21	38.39384975657854	40.0	38.0	41.0	34.0	41.0
22	38.13298822949405	40.0	38.0	41.0	33.0	41.0
23	38.13687064768595	40.0	37.0	41.0	33.0	41.0
24	37.91125901275652	40.0	37.0	41.0	33.0	41.0
25	37.847784556603195	40.0	37.0	41.0	33.0	41.0
26	37.73562580883712	40.0	37.0	41.0	33.0	41.0
27	37.342207432057684	39.0	36.0	41.0	31.0	41.0
28	37.39181610895421	39.0	36.0	41.0	31.0	41.0
29	37.23473223639613	39.0	36.0	41.0	31.0	41.0
30	37.09662907499846	39.0	36.0	41.0	31.0	41.0
31	37.09724533185432	39.0	36.0	41.0	31.0	41.0
32	36.7586738152462	39.0	35.0	40.0	30.0	41.0
33	36.60023417760522	39.0	35.0	40.0	30.0	41.0
34	36.57250261909164	39.0	35.0	40.0	30.0	41.0
35	36.42725087816602	39.0	35.0	40.0	30.0	41.0
36	36.496949528563505	39.0	35.0	40.0	30.0	41.0
37	36.40420287175695	39.0	35.0	40.0	30.0	41.0
38	36.19325814999692	39.0	35.0	40.0	29.0	41.0
39	36.04350773402354	39.0	35.0	40.0	29.0	41.0
40	35.61995439699267	38.0	34.0	40.0	27.0	41.0
41	35.60843039378813	38.0	35.0	40.0	27.0	41.0
42	35.38004560300733	38.0	34.0	40.0	26.0	41.0
43	34.45904973192827	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	0.0
19	2.0
20	2.0
21	9.0
22	11.0
23	27.0
24	45.0
25	69.0
26	101.0
27	145.0
28	199.0
29	250.0
30	321.0
31	410.0
32	535.0
33	688.0
34	822.0
35	1117.0
36	1530.0
37	2407.0
38	3840.0
39	3693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.74573242127319	19.073149688790288	16.176742466259938	27.004375423676592
2	16.626609971035926	20.299500831946755	35.63813397424047	27.43575522277685
3	17.674246625993714	24.22505700375917	36.605657237936775	21.49503913231035
4	11.70271769273433	18.573981635545696	27.454242928452576	42.269057743267396
5	12.152585197510323	35.68743452270907	34.541196770814075	17.618783508966537
6	31.361311394589265	31.99605595612251	16.152092192025634	20.490540457262586
7	23.534849325198742	33.7400628581993	16.916250693288962	25.808837123312994
8	31.97140568188821	29.383126887286622	20.108461206630924	18.537006224194243
9	23.306834288531462	11.388426696246997	15.622111295988168	49.68262771923337
10	18.894435200591605	25.944413631601655	24.964565230788192	30.19658593701855
11	31.47223762864362	19.726381955999262	18.198064953472606	30.603315461884513
12	19.49836691933198	31.47223762864362	21.149935293030133	27.879460158994267
13	33.92493991495655	15.22154433968078	24.625623960066555	26.22789178529611
14	25.2850187958341	22.641276884205336	20.866457139335676	31.207247180624886
15	29.28452579034942	24.471559746102177	18.290503481851236	27.953410981697168
16	31.231897454859187	21.365625192580268	20.847969433659948	26.5545079189006
17	18.111788993652556	36.34066678991804	20.059160658162323	25.488383558267085
18	27.429592654218276	18.63560732113145	22.733715412583965	31.20108461206631
19	26.98588771800086	27.03518826646946	21.790842423121955	24.188081592407716
20	24.385283786282123	19.57231774203488	24.144943612497688	31.89745485918531
21	35.249892155050226	18.56781906698712	21.41492574104887	24.767363036913785
22	23.22055832871141	27.793184199174213	25.69174832070007	23.294509151414307
23	27.768533924939913	23.762864361866026	20.8849448450114	27.58365686818266
24	31.583163862697972	23.34380969988291	25.06932889628397	20.003697541135146
25	21.29783693843594	26.634621310162075	26.301842607999014	25.76569914340297
26	32.821840142971595	24.89061440808529	21.729216737536206	20.558328711406915
27	20.213224872126702	29.074998459357857	26.690084427189255	24.021692241326186
28	23.214395760152833	23.57798730510877	27.213902754668144	25.993714180070253
29	25.86430024034017	21.69840389474333	27.817834473408514	24.61946139150798
30	19.658593701854933	23.251371171504285	32.26720897269983	24.822826153940962
31	27.80550933629137	23.941578850064708	21.957231774203485	26.295680039440438
32	22.555000924385286	22.91859246934122	33.659949466937825	20.866457139335676
33	19.707894250323534	27.787021630615637	30.837493067110373	21.667591051950453
34	24.92758981943674	26.93658716953226	24.976890367905344	23.158932643125656
35	19.658593701854933	21.932581499969185	37.48074197325445	20.928082824921425
36	23.701238676280273	27.004375423676592	22.54883835582671	26.74554754421643
37	21.29783693843594	24.884451839526715	27.639119985209838	26.178591236827508
38	21.112959881678684	20.8356442965428	29.524865964133852	28.526529857644668
39	25.426757872681332	22.69057743267394	25.11862944475257	26.764035249892153
40	18.931410611943058	19.331977568250448	37.3205151907315	24.416096629075
41	21.18074813582301	22.2776853392494	26.04917729709743	30.49238922783016
42	21.211560978615886	19.473716645097678	35.718247365501945	23.596475010784495
43	19.356627842484748	20.804831453749923	33.34565847045049	26.492882233314845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	4.5
4	6.0
5	6.0
6	13.0
7	20.0
8	24.5
9	29.0
10	47.5
11	66.0
12	66.0
13	91.5
14	117.0
15	186.5
16	256.0
17	226.5
18	197.0
19	197.0
20	207.5
21	218.0
22	158.0
23	98.0
24	101.5
25	105.0
26	105.0
27	114.0
28	123.0
29	153.5
30	184.0
31	201.5
32	219.0
33	219.0
34	266.5
35	314.0
36	323.0
37	332.0
38	402.0
39	472.0
40	472.0
41	533.5
42	595.0
43	636.5
44	678.0
45	788.5
46	899.0
47	899.0
48	1092.0
49	1285.0
50	1789.5
51	2294.0
52	1651.5
53	1009.0
54	1009.0
55	1506.0
56	2003.0
57	1756.5
58	1510.0
59	1138.0
60	766.0
61	766.0
62	707.5
63	649.0
64	578.0
65	507.0
66	459.5
67	412.0
68	412.0
69	350.5
70	289.0
71	264.5
72	240.0
73	197.5
74	155.0
75	155.0
76	117.5
77	80.0
78	66.0
79	52.0
80	38.0
81	24.0
82	24.0
83	17.5
84	11.0
85	9.0
86	7.0
87	4.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	16227.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.55808220866457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.37744182096144	68.47846182288778
2	3.7880074740954646	5.4970111542490905
3	0.7898759979616103	1.7193566278424846
4	0.37370477322914897	1.0846120663092378
5	0.20383896721589945	0.7395082270290257
6	0.05095974180397486	0.22185246810870773
7	0.08493290300662477	0.431379799100265
8	0.016986580601324953	0.09860109693720343
9	0.02547987090198743	0.16638935108153077
>10	0.19534567691523697	2.4650274234300857
>50	0.016986580601324953	1.0722869291920873
>100	0.05945303210463733	9.724533185431689
>500	0.016986580601324953	8.300979848400814
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	706	4.3507734023541005	TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	641	3.9502064460467126	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	379	2.335613483700006	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	353	2.1753867011770502	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	328	2.0213224872126703	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	152	0.9367104209034326	RNA PCR Primer, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.8689221667591053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.745670795587601	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	104	0.6409071300918222	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.5484686017131941	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC	85	0.5238183274788931	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.28347815369445983	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	35	0.2156898995501325	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	33	0.20336476243298204	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	28	0.172551919640106	No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT	22	0.13557650828865472	TruSeq Adapter, Index 12 (95% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	19	0.11708880261292907	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	19	0.11708880261292907	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	17	0.10476366549577863	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	17	0.10476366549577863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.024650274234300856	0.0
3	0.0	0.0	0.0	0.04930054846860171	0.0
4	0.0	0.0	0.0	0.08011339126147779	0.0
5	0.0	0.0	0.0	0.09243852837862822	0.0
6	0.0	0.0	0.0	0.09243852837862822	0.0
7	0.0	0.0	0.0	0.09243852837862822	0.0
8	0.0	0.0	0.0	0.09860109693720343	0.0
9	0.0	0.0	0.0	0.16022678252295558	0.0
10	0.0	0.0	0.0	0.24034017378443334	0.0
11	0.0	0.0	0.0	0.24650274234300856	0.0
12	0.0	0.0	0.0	0.2649904480187342	0.0
13	0.0	0.0	0.0	0.27115301657730945	0.0
14	0.0	0.0	0.0	0.2773155851358846	0.0
15	0.0	0.0	0.0	0.3081284279287607	0.0
16	0.0	0.0	0.0	0.3081284279287607	0.0
17	0.0	0.0	0.0	0.33894127072163677	0.0
18	0.0	0.0	0.0	0.345103839280212	0.0
19	0.0	0.0	0.0	0.36359154495593765	0.0
20	0.0	0.0	0.0	0.36359154495593765	0.0
21	0.0	0.0	0.0	0.41289209342453936	0.0
22	0.0	0.0	0.0	0.4868429161274419	0.0
23	0.0	0.0	0.0	0.5546311702717692	0.0
24	0.0	0.0	0.0	0.6532322672089727	0.0
25	0.0	0.0	0.0	0.6840451100018488	0.0
26	0.0	0.0	0.0	0.7086953842361496	0.0
27	0.0	0.0	0.0	0.745670795587601	0.0
28	0.0	0.0	0.0	0.8504344610833795	0.0
29	0.0	0.0	0.0	0.9120601466691317	0.0
30	0.0	0.0	0.0	1.0846120663092378	0.0
31	0.0	0.0	0.0	1.6084303937881308	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	30	7.817345E-6	37.0	37
TTATACA	95	0.0	27.263159	2
CCGTCTT	105	0.0	26.42857	37
GCCGTCT	105	0.0	26.42857	36
TATACAC	100	0.0	25.899998	3
CTTATAC	100	0.0	25.899998	1
TGCCGTC	140	3.6379788E-12	19.821428	35
ATGCCGT	145	5.456968E-12	19.13793	34
TATGCCG	160	1.8189894E-12	18.5	33
TACACAT	145	1.1641532E-10	17.862068	5
ATACACA	145	1.1641532E-10	17.862068	4
GTATGCC	195	2.910383E-11	15.179487	32
CGTATGC	195	2.910383E-11	15.179487	31
ACACATC	180	2.5047484E-9	14.388888	6
ACATCTC	195	7.741619E-9	13.282052	8
CACATCT	200	1.1048542E-8	12.949999	7
TCGTATG	245	1.0895747E-9	12.081634	30
CTGTCTC	125	6.567583E-4	11.840001	37
TCTCGTA	255	2.048182E-9	11.607844	28
CTCGTAT	255	2.048182E-9	11.607844	29
>>END_MODULE