FastQCFastQC Report
Fri 10 Feb 2017
ERR1630744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622000
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG17620.28327974276527335No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG15480.24887459807073956No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT12390.19919614147909967No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12290.19758842443729904No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC9800.15755627009646303No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG9100.14630225080385853No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC8870.14260450160771704No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC8840.14212218649517686No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8210.1319935691318328No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG7500.12057877813504825No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC7320.11768488745980707No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC7210.11591639871382636No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC7020.11286173633440515No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA6870.11045016077170418No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA6670.1072347266881029No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA6610.1062700964630225No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT6540.10514469453376206No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT6250.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGAT556.253995E-726.9090914
CCTACAC753.7350583E-722.23
TAGACAG603.722749E-521.5833325
CAAGACG1153.0431693E-919.3043464
GCGGGTA1457.2759576E-1219.1379323
CGGGTAA1501.2732926E-1118.524
AACCGAC500.007031341518.57
AACCCGT1409.276846E-1118.529
GCAGTCG801.6147269E-518.59
ACCCTAT500.007031341518.51
AAACCGA609.2303223E-418.56
TTGCGCG902.1489868E-618.518
GCCGTTT609.2303223E-418.515
GTCGGGA1002.869656E-718.52
AGTCGGT801.6147269E-518.511
AACACCC609.2303223E-418.55
GGTATCA2800.017.8392871
GTTCAAA1258.556526E-917.761
TCTATGG1258.556526E-917.762
TATACAC2300.017.695653
Produced by FastQC (version 0.11.5)