##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630744.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622000
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08083922829582	33.0	31.0	34.0	30.0	34.0
2	32.276418006430866	34.0	31.0	34.0	30.0	34.0
3	32.37714469453376	34.0	31.0	34.0	30.0	34.0
4	35.95007395498392	37.0	35.0	37.0	35.0	37.0
5	35.907443729903534	37.0	35.0	37.0	35.0	37.0
6	35.981128617363346	37.0	35.0	37.0	35.0	37.0
7	35.931627009646306	37.0	35.0	37.0	35.0	37.0
8	35.92145980707396	37.0	35.0	37.0	35.0	37.0
9	37.66948231511254	39.0	37.0	39.0	35.0	39.0
10	37.579792604501606	39.0	37.0	39.0	35.0	39.0
11	37.67008360128617	39.0	37.0	39.0	35.0	39.0
12	37.60744372990354	39.0	37.0	39.0	35.0	39.0
13	37.62873311897106	39.0	37.0	39.0	35.0	39.0
14	38.960147909967844	40.0	38.0	41.0	36.0	41.0
15	38.99552893890675	40.0	38.0	41.0	36.0	41.0
16	38.95327009646302	40.0	38.0	41.0	36.0	41.0
17	38.91709163987138	40.0	38.0	41.0	35.0	41.0
18	38.88506270096463	40.0	38.0	41.0	35.0	41.0
19	38.95497266881029	40.0	38.0	41.0	35.0	41.0
20	38.91918327974277	40.0	38.0	41.0	35.0	41.0
21	38.87859967845659	40.0	38.0	41.0	35.0	41.0
22	38.821186495176846	40.0	38.0	41.0	35.0	41.0
23	38.774961414790994	40.0	38.0	41.0	35.0	41.0
24	38.73489710610932	40.0	38.0	41.0	35.0	41.0
25	38.684861736334405	40.0	38.0	41.0	34.0	41.0
26	38.574371382636656	40.0	38.0	41.0	34.0	41.0
27	38.453514469453374	40.0	38.0	41.0	34.0	41.0
28	38.37455627009646	40.0	38.0	41.0	34.0	41.0
29	38.31325723472669	40.0	38.0	41.0	34.0	41.0
30	38.26314308681672	40.0	38.0	41.0	34.0	41.0
31	38.25362861736335	40.0	38.0	41.0	34.0	41.0
32	38.168617363344055	40.0	38.0	41.0	33.0	41.0
33	38.121602893890675	40.0	38.0	41.0	33.0	41.0
34	38.09784887459807	40.0	38.0	41.0	33.0	41.0
35	38.052630225080385	40.0	38.0	41.0	33.0	41.0
36	38.04841961414791	40.0	38.0	41.0	33.0	41.0
37	37.94566077170418	40.0	37.0	41.0	33.0	41.0
38	37.81337942122187	40.0	37.0	41.0	33.0	41.0
39	37.79893729903537	40.0	37.0	41.0	33.0	41.0
40	37.66741800643087	40.0	37.0	41.0	33.0	41.0
41	37.65199678456592	40.0	37.0	41.0	33.0	41.0
42	37.55426688102894	40.0	37.0	41.0	32.0	41.0
43	36.73739710610933	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	8.0
18	12.0
19	27.0
20	53.0
21	97.0
22	232.0
23	367.0
24	681.0
25	1080.0
26	1711.0
27	2551.0
28	3723.0
29	5320.0
30	7419.0
31	10008.0
32	13274.0
33	17450.0
34	23649.0
35	32122.0
36	45724.0
37	70818.0
38	138832.0
39	246830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.396302250803856	17.728295819935692	12.0967845659164	26.778617363344054
2	19.347588424437298	20.39967845659164	34.044533762057874	26.20819935691318
3	20.486816720257234	21.782797427652735	29.462540192926046	28.26784565916399
4	15.19967845659164	14.861575562700965	32.563022508038586	37.37572347266881
5	15.068810289389067	36.737781350482315	33.561254019292605	14.632154340836012
6	37.19389067524116	33.12684887459807	14.849196141479098	14.830064308681672
7	30.522025723472666	29.66511254019293	19.885209003215433	19.927652733118972
8	27.520900321543408	33.61897106109325	19.474276527331188	19.385852090032156
9	26.774115755627008	13.7524115755627	17.832797427652732	41.64067524115755
10	17.06768488745981	26.987781350482315	31.987781350482315	23.956752411575565
11	37.311414790996785	20.3967845659164	20.493890675241158	21.79790996784566
12	22.60209003215434	24.719453376205788	27.157395498392283	25.52106109324759
13	31.239549839228296	18.380225080385852	24.419453376205787	25.960771704180065
14	23.793729903536978	19.70209003215434	22.808199356913185	33.6959807073955
15	26.82411575562701	27.147427652733118	20.82733118971061	25.201125401929257
16	27.420257234726687	25.016398713826366	21.75755627009646	25.80578778135048
17	25.794855305466236	25.52395498392283	22.95273311897106	25.72845659163987
18	24.767363344051446	23.98118971061093	25.029581993569135	26.221864951768488
19	26.491961414791	24.487299035369777	23.941318327974276	25.07942122186495
20	26.382797427652733	23.5016077170418	24.639389067524114	25.476205787781353
21	26.390996784565917	24.3991961414791	23.184244372990353	26.025562700964635
22	26.361575562700967	23.77186495176849	23.2548231511254	26.611736334405144
23	25.192122186495176	24.568327974276528	23.87909967845659	26.360450160771702
24	26.934083601286176	24.62073954983923	23.32459807073955	25.120578778135048
25	26.529742765273312	24.09308681672026	23.632958199356914	25.74421221864952
26	26.819131832797428	24.15353697749196	23.329742765273313	25.6975884244373
27	26.572186495176847	23.481672025723473	23.716077170418007	26.230064308681673
28	25.5475884244373	23.711254019292603	24.421704180064307	26.319453376205786
29	25.129421221864952	24.778456591639873	23.990675241157554	26.10144694533762
30	24.79774919614148	25.72684887459807	23.88151125401929	25.59389067524116
31	26.311736334405143	24.3056270096463	23.655787781350483	25.72684887459807
32	24.991479099678457	24.310128617363343	24.169453376205787	26.528938906752416
33	25.279260450160773	23.75273311897106	24.2491961414791	26.718810289389065
34	25.886173633440514	23.84212218649518	24.309807073954985	25.961897106109326
35	25.632958199356914	23.760450160771704	24.64871382636656	25.95787781350482
36	25.064308681672028	24.65787781350482	24.47202572347267	25.80578778135048
37	26.471221864951765	23.086977491961413	24.270900321543408	26.17090032154341
38	25.0475884244373	23.480707395498392	25.829742765273313	25.641961414790998
39	25.555305466237943	23.038424437299035	25.425241157556272	25.981028938906753
40	24.61832797427653	22.650803858520902	26.73118971061093	25.999678456591642
41	24.053536977491962	23.14131832797428	26.878938906752413	25.92620578778135
42	25.05257234726688	22.3975884244373	26.607395498392282	25.942443729903538
43	23.704019292604503	22.423794212218652	27.074276527331186	26.797909967845655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.5
2	6.0
3	23.0
4	40.0
5	40.0
6	67.0
7	94.0
8	99.5
9	105.0
10	141.0
11	177.0
12	177.0
13	301.5
14	426.0
15	729.0
16	1032.0
17	1042.5
18	1053.0
19	1053.0
20	1247.5
21	1442.0
22	1682.0
23	1922.0
24	2444.0
25	2966.0
26	2966.0
27	3633.0
28	4300.0
29	6659.5
30	9019.0
31	10003.0
32	10987.0
33	10987.0
34	13171.0
35	15355.0
36	17076.0
37	18797.0
38	24165.5
39	29534.0
40	29534.0
41	32551.5
42	35569.0
43	34450.5
44	33332.0
45	37484.0
46	41636.0
47	41636.0
48	45886.0
49	50136.0
50	53160.0
51	56184.0
52	60974.5
53	65765.0
54	65765.0
55	57302.0
56	48839.0
57	46073.0
58	43307.0
59	39840.0
60	36373.0
61	36373.0
62	34052.5
63	31732.0
64	28656.0
65	25580.0
66	22552.0
67	19524.0
68	19524.0
69	16589.0
70	13654.0
71	11766.0
72	9878.0
73	7788.0
74	5698.0
75	5698.0
76	4453.0
77	3208.0
78	2642.0
79	2076.0
80	1630.5
81	1185.0
82	1185.0
83	857.5
84	530.0
85	414.0
86	298.0
87	225.0
88	152.0
89	152.0
90	112.0
91	72.0
92	40.0
93	8.0
94	5.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	622000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.10382676748553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.38410533307687	51.04174466706702
2	6.263749596115551	7.1536814370298005
3	1.5078912640949242	2.5831908458724397
4	0.6927404926272726	1.5823253234324146
5	0.3587936949684386	1.0244246501371883
6	0.25181528913262247	0.8627769988820134
7	0.17024589072881838	0.6805184296438301
8	0.1428151906814381	0.6524235126750605
9	0.11019450037935252	0.5663274894352949
>10	0.8314004770448746	9.878373899205663
>50	0.15044939141005118	5.98233172170936
>100	0.1273466673078688	14.274965077466964
>500	0.007325250774346325	2.7873249703263143
>1k	0.0011269616575917425	0.9295909771166202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1762	0.28327974276527335	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1548	0.24887459807073956	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1239	0.19919614147909967	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1229	0.19758842443729904	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	980	0.15755627009646303	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	910	0.14630225080385853	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	887	0.14260450160771704	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	884	0.14212218649517686	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	821	0.1319935691318328	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	750	0.12057877813504825	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	732	0.11768488745980707	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	721	0.11591639871382636	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	702	0.11286173633440515	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	687	0.11045016077170418	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	667	0.1072347266881029	No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA	661	0.1062700964630225	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	654	0.10514469453376206	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	625	0.10048231511254019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0012861736334405145	0.0
3	0.0	0.0	0.0	0.002411575562700965	0.0
4	0.0	0.0	0.0	0.0038585209003215433	0.0
5	0.0	0.0	0.0	0.004340836012861737	0.0
6	0.0	0.0	0.0	0.0045016077170418	0.0
7	0.0	0.0	0.0	0.0045016077170418	0.0
8	0.0	0.0	0.0	0.005305466237942123	0.0
9	0.0	0.0	0.0	0.010289389067524116	0.0
10	0.0	0.0	0.0	0.015112540192926046	0.0
11	0.0	0.0	0.0	0.017684887459807074	0.0
12	0.0	0.0	0.0	0.01929260450160772	0.0
13	0.0	0.0	0.0	0.020739549839228297	0.0
14	0.0	0.0	0.0	0.021061093247588425	0.0
15	0.0	0.0	0.0	0.021864951768488745	0.0
16	0.0	1.607717041800643E-4	0.0	0.023633440514469452	0.0
17	0.0	1.607717041800643E-4	0.0	0.025241157556270096	0.0
18	0.0	1.607717041800643E-4	0.0	0.025562700964630224	0.0
19	0.0	1.607717041800643E-4	0.0	0.02684887459807074	0.0
20	0.0	1.607717041800643E-4	0.0	0.02829581993569132	0.0
21	0.0	1.607717041800643E-4	0.0	0.030707395498392284	0.0
22	0.0	1.607717041800643E-4	0.0	0.03376205787781351	0.0
23	0.0	1.607717041800643E-4	0.0	0.03810289389067524	0.0
24	0.0	1.607717041800643E-4	0.0	0.04630225080385852	0.0
25	0.0	1.607717041800643E-4	0.0	0.04967845659163987	0.0
26	0.0	1.607717041800643E-4	0.0	0.05176848874598071	0.0
27	0.0	1.607717041800643E-4	0.0	0.05691318327974276	0.0
28	0.0	1.607717041800643E-4	0.0	0.07684887459807074	0.0
29	0.0	1.607717041800643E-4	0.0	0.10964630225080386	0.0
30	0.0	1.607717041800643E-4	0.0	0.15514469453376206	0.0
31	0.0	1.607717041800643E-4	0.0	0.3339228295819936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGAT	55	6.253995E-7	26.90909	14
CCTACAC	75	3.7350583E-7	22.2	3
TAGACAG	60	3.722749E-5	21.583332	5
CAAGACG	115	3.0431693E-9	19.304346	4
GCGGGTA	145	7.2759576E-12	19.13793	23
CGGGTAA	150	1.2732926E-11	18.5	24
AACCGAC	50	0.0070313415	18.5	7
AACCCGT	140	9.276846E-11	18.5	29
GCAGTCG	80	1.6147269E-5	18.5	9
ACCCTAT	50	0.0070313415	18.5	1
AAACCGA	60	9.2303223E-4	18.5	6
TTGCGCG	90	2.1489868E-6	18.5	18
GCCGTTT	60	9.2303223E-4	18.5	15
GTCGGGA	100	2.869656E-7	18.5	2
AGTCGGT	80	1.6147269E-5	18.5	11
AACACCC	60	9.2303223E-4	18.5	5
GGTATCA	280	0.0	17.839287	1
GTTCAAA	125	8.556526E-9	17.76	1
TCTATGG	125	8.556526E-9	17.76	2
TATACAC	230	0.0	17.69565	3
>>END_MODULE