FastQCFastQC Report
Fri 10 Feb 2017
ERR1630743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378429
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70380.5105812486533583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62730.4550832868432106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50640.36737474327658515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32950.23904024073782545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCC951.6767262E-719.4736843
TCTGTCG1306.9667294E-1018.58
GCAGTCG3100.017.9032259
TATACTG1156.407572E-817.6956525
CGAATTA1201.0416079E-716.95833415
CCCCTAG1005.8795013E-616.651
CAGTCGG3000.016.6510
ATTATAC1452.9813236E-916.5862063
TCGGTGA3350.016.56716313
CGAACGA1352.2202585E-816.44444316
ACGAACG1352.2202585E-816.44444315
GCTTAGG2850.016.2280711
TAGGACA2850.016.2280714
ACTATAC1751.3096724E-1015.8571433
GGTATCA33350.015.5877061
AGTCGGT3300.015.13636511
CGGTGAT3550.015.11267614
CGATAAC1601.0966687E-815.03125110
GTCGGTG3450.015.01449312
TAGACTA750.004104957514.85
Produced by FastQC (version 0.11.5)