##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630743.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1378429
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1029258670559	33.0	31.0	34.0	30.0	34.0
2	32.27513713074812	34.0	31.0	34.0	30.0	34.0
3	32.37615793051365	34.0	31.0	34.0	30.0	34.0
4	35.949069556720005	37.0	35.0	37.0	35.0	37.0
5	35.91270351973152	37.0	35.0	37.0	35.0	37.0
6	35.98709472885437	37.0	35.0	37.0	35.0	37.0
7	35.93612075776119	37.0	35.0	37.0	35.0	37.0
8	35.9381411737565	37.0	35.0	37.0	35.0	37.0
9	37.661541508485385	39.0	37.0	39.0	35.0	39.0
10	37.56940546085435	39.0	37.0	39.0	35.0	39.0
11	37.670346459629044	39.0	37.0	39.0	35.0	39.0
12	37.61954732525215	39.0	37.0	39.0	35.0	39.0
13	37.658261687761936	39.0	37.0	39.0	35.0	39.0
14	38.96132408705853	40.0	38.0	41.0	36.0	41.0
15	38.972044987445855	40.0	38.0	41.0	36.0	41.0
16	38.93904002309876	40.0	38.0	41.0	36.0	41.0
17	38.929163562287215	40.0	38.0	41.0	36.0	41.0
18	38.91885762705225	40.0	38.0	41.0	35.0	41.0
19	38.98333247486813	40.0	38.0	41.0	35.0	41.0
20	38.95435020592283	40.0	38.0	41.0	35.0	41.0
21	38.914467121629045	40.0	38.0	41.0	35.0	41.0
22	38.846682709084035	40.0	38.0	41.0	35.0	41.0
23	38.79130372329659	40.0	38.0	41.0	35.0	41.0
24	38.74417180718049	40.0	38.0	41.0	35.0	41.0
25	38.688388012730435	40.0	38.0	41.0	34.0	41.0
26	38.56026316915851	40.0	38.0	41.0	34.0	41.0
27	38.43360448742735	40.0	38.0	41.0	34.0	41.0
28	38.340574668698935	40.0	38.0	41.0	34.0	41.0
29	38.26456495038917	40.0	38.0	41.0	34.0	41.0
30	38.182427241446604	40.0	38.0	41.0	34.0	41.0
31	38.12011064770112	40.0	37.0	41.0	33.0	41.0
32	38.00991563584341	40.0	37.0	41.0	33.0	41.0
33	37.92890602272587	40.0	37.0	41.0	33.0	41.0
34	37.858035488226086	40.0	37.0	41.0	33.0	41.0
35	37.78468894661967	40.0	37.0	41.0	33.0	41.0
36	37.74303500579283	40.0	37.0	41.0	33.0	41.0
37	37.60434958927881	40.0	37.0	41.0	33.0	41.0
38	37.44879932154649	40.0	37.0	41.0	32.0	41.0
39	37.38240489716917	40.0	36.0	41.0	32.0	41.0
40	37.206884068747826	40.0	36.0	41.0	31.0	41.0
41	37.13671288111321	40.0	36.0	41.0	31.0	41.0
42	36.99477521149076	40.0	36.0	41.0	31.0	41.0
43	36.2061919765182	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	6.0
12	5.0
13	7.0
14	1.0
15	6.0
16	10.0
17	12.0
18	36.0
19	73.0
20	174.0
21	332.0
22	591.0
23	1032.0
24	1659.0
25	2806.0
26	4211.0
27	6498.0
28	9612.0
29	13499.0
30	18225.0
31	24075.0
32	31151.0
33	40786.0
34	53738.0
35	72653.0
36	102761.0
37	164393.0
38	299835.0
39	530234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01293066236999	19.020421073555475	12.263961364713017	24.70268689936152
2	18.51049274209988	21.176208567869654	35.13108038208714	25.182218307943316
3	20.16077723263222	23.62617153295527	29.66079500648927	26.552256227923237
4	14.120930421516087	15.450705114300412	34.422520129799935	36.00584433438356
5	14.008556117144952	37.700599740719326	34.09352240848096	14.19732173365476
6	34.51617747450177	34.65220189070311	16.05385551232599	14.77776512246913
7	28.81272811294597	30.83481267442864	21.587473856107206	18.76498535651818
8	27.429269117234185	33.37415274925296	20.167886775452345	19.028691358060517
9	26.806168471499074	13.41382109633503	20.21214005218985	39.56787037997604
10	16.736734354834383	27.27249644341493	33.151072706682754	22.839696495067933
11	34.882971846935895	22.044588440899023	21.732711659432585	21.339728052732497
12	21.346039585644235	26.013744632476538	29.57664123433271	23.06357454754652
13	29.958597795026076	19.92746815396368	25.93198489004512	24.18194916096513
14	22.88482032806913	21.115559814832682	26.107909801665517	29.891710055432668
15	25.596893274880316	27.776911251867165	23.093898924065005	23.532296549187517
16	24.789089608532613	26.496395534336553	24.80004410818403	23.91447074894681
17	22.99530842720227	26.90287276312382	26.343975641835744	23.757843167838168
18	22.869440500743963	25.226906862812665	27.869480401239382	24.03417223520399
19	23.680146021303962	25.754318865897336	27.950369587407113	22.615165525391586
20	24.31209732238657	24.808677124465607	27.854028027558908	23.025197525588915
21	24.502821690489682	25.33093833632345	26.83707321886002	23.329166754326845
22	24.351272354252558	25.28668505958595	26.80029221671918	23.561750369442315
23	23.510460096239996	25.53631706819865	27.196105131276255	23.757117704285097
24	23.548111654644526	25.917403072628332	27.11173372005377	23.42275155267337
25	23.770974058148806	25.487565917432093	27.029828884911737	23.711631139507368
26	23.481731739538272	26.009899675645244	27.161137788018095	23.347230796798385
27	23.936742480026176	25.563957229570768	26.7730147871236	23.726285503279456
28	23.189732659426056	25.854577928932137	27.52691651147792	23.42877290016388
29	22.85493122968249	26.173926984995237	27.655105921306067	23.31603586401621
30	22.76410319283764	26.844908225233215	27.47627915547337	22.914709426455772
31	23.36144988243863	26.527445374408114	26.817122971150493	23.293981772002763
32	22.627425859438535	26.36733556824472	27.22062579937015	23.78461277294659
33	22.65027796136036	26.052629478921297	27.544545275817615	23.75254728390073
34	23.404614963846523	25.80270728488736	27.19516202865726	23.597515722608854
35	23.269823835685408	25.97072464377926	27.657282311965286	23.102169208570047
36	22.68524530461852	26.288840411802134	27.326833663540164	23.69908062003919
37	23.379078646778325	25.352484603849746	27.48034175137058	23.78809499800135
38	22.68002196703639	25.499898797834348	28.128035611554893	23.692043623574374
39	22.7096208800018	25.417123406428622	28.093576092783884	23.77967962078569
40	22.287110906691606	25.286467420520026	28.800322686188405	23.626098986599963
41	21.617580593559772	25.498738056149428	29.118075722434746	23.76560562785606
42	22.185255823840038	25.261584020649597	28.80917334153591	23.74398681397446
43	21.42076233161084	25.012169651102813	29.123589245438104	24.44347877184824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	169.0
2	179.0
3	630.0
4	1081.0
5	1081.0
6	1522.0
7	1963.0
8	1992.5
9	2022.0
10	2854.5
11	3687.0
12	3687.0
13	6194.0
14	8701.0
15	12506.0
16	16311.0
17	15518.0
18	14725.0
19	14725.0
20	16701.0
21	18677.0
22	17522.0
23	16367.0
24	18371.0
25	20375.0
26	20375.0
27	23334.0
28	26293.0
29	30944.5
30	35596.0
31	39795.5
32	43995.0
33	43995.0
34	48296.5
35	52598.0
36	57131.5
37	61665.0
38	67032.0
39	72399.0
40	72399.0
41	76089.0
42	79779.0
43	80255.5
44	80732.0
45	84396.0
46	88060.0
47	88060.0
48	90219.0
49	92378.0
50	93828.5
51	95279.0
52	98381.5
53	101484.0
54	101484.0
55	95075.5
56	88667.0
57	85181.5
58	81696.0
59	74991.5
60	68287.0
61	68287.0
62	63421.5
63	58556.0
64	51809.0
65	45062.0
66	39266.5
67	33471.0
68	33471.0
69	28909.0
70	24347.0
71	20913.5
72	17480.0
73	14129.0
74	10778.0
75	10778.0
76	8565.5
77	6353.0
78	5168.0
79	3983.0
80	3138.5
81	2294.0
82	2294.0
83	1736.0
84	1178.0
85	1014.5
86	851.0
87	729.0
88	607.0
89	607.0
90	443.5
91	280.0
92	151.5
93	23.0
94	14.5
95	6.0
96	6.0
97	4.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1378429.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.08692796106857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.651859537135	52.93295901237598
2	8.115151538705659	9.914593548761347
3	2.0284151679519122	3.7172895371945183
4	0.8744128506612862	2.1366077926631437
5	0.4890340816373667	1.4936794857744575
6	0.3164029653567367	1.1596851090849267
7	0.22020749787982477	0.9416259691630412
8	0.15863882809068197	0.7752606930723065
9	0.12477815633908289	0.6860082822660457
>10	0.830590027978987	9.878198066883863
>50	0.10647111793990792	4.583630876749545
>100	0.08059113865489229	9.070106447090662
>500	0.002971630748850792	1.1368722650187226
>1k	1.1886522995403168E-4	0.239253631809203
>5k	3.5659568986209504E-4	1.3342292820922927
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7038	0.5105812486533583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6273	0.4550832868432106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5064	0.36737474327658515	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3295	0.23904024073782545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.078244871516778E-4	0.0
3	0.0	0.0	0.0	0.0012332880402255032	0.0
4	0.0	0.0	0.0	0.0022489370145288585	0.0
5	0.0	0.0	0.0	0.0023940297251436237	0.0
6	0.0	0.0	0.0	0.0024665760804510064	0.0
7	0.0	0.0	0.0	0.002684215146373154	0.0
8	0.0	0.0	0.0	0.0027567615016805365	0.0
9	0.0	0.0	0.0	0.0047880594502872475	0.0
10	0.0	0.0	0.0	0.007544820951967784	0.0
11	0.0	0.0	0.0	0.009285933479344964	0.0
12	0.0	7.254635530738253E-5	0.0	0.010156489743033554	0.0
13	0.0	7.254635530738253E-5	0.0	0.010809406940799998	0.0
14	0.0	7.254635530738253E-5	0.0	0.012115241336332884	0.0
15	0.0	7.254635530738253E-5	0.0	0.0137112611530953	0.0
16	0.0	7.254635530738253E-5	0.0	0.017411125273771807	0.0
17	0.0	7.254635530738253E-5	0.0	0.022779555566518117	0.0
18	0.0	7.254635530738253E-5	0.0	0.025391224357583887	0.0
19	0.0	7.254635530738253E-5	0.0	0.028728356701723485	0.0
20	0.0	7.254635530738253E-5	0.0	0.031049840071559726	0.0
21	0.0	7.254635530738253E-5	0.0	0.03699864120676509	0.0
22	0.0	7.254635530738253E-5	0.0	0.04998443880678657	0.0
23	0.0	7.254635530738253E-5	0.0	0.06848375941016911	0.0
24	0.0	7.254635530738253E-5	0.0	0.09786503330965904	0.0
25	0.0	7.254635530738253E-5	0.0	0.1050471224850899	0.0
26	0.0	7.254635530738253E-5	0.0	0.1162918075577342	0.0
27	0.0	7.254635530738253E-5	0.0	0.13138144946166977	0.0
28	0.0	7.254635530738253E-5	0.0	0.16083526971646708	0.0
29	0.0	7.254635530738253E-5	0.0	0.20871586421933955	0.0
30	0.0	7.254635530738253E-5	0.0	0.2835111565412509	0.0
31	0.0	7.254635530738253E-5	0.0	0.5627420781193663	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTCC	95	1.6767262E-7	19.473684	3
TCTGTCG	130	6.9667294E-10	18.5	8
GCAGTCG	310	0.0	17.903225	9
TATACTG	115	6.407572E-8	17.695652	5
CGAATTA	120	1.0416079E-7	16.958334	15
CCCCTAG	100	5.8795013E-6	16.65	1
CAGTCGG	300	0.0	16.65	10
ATTATAC	145	2.9813236E-9	16.586206	3
TCGGTGA	335	0.0	16.567163	13
CGAACGA	135	2.2202585E-8	16.444443	16
ACGAACG	135	2.2202585E-8	16.444443	15
GCTTAGG	285	0.0	16.228071	1
TAGGACA	285	0.0	16.228071	4
ACTATAC	175	1.3096724E-10	15.857143	3
GGTATCA	3335	0.0	15.587706	1
AGTCGGT	330	0.0	15.136365	11
CGGTGAT	355	0.0	15.112676	14
CGATAAC	160	1.0966687E-8	15.031251	10
GTCGGTG	345	0.0	15.014493	12
TAGACTA	75	0.0041049575	14.8	5
>>END_MODULE