FastQCFastQC Report
Fri 10 Feb 2017
ERR1630742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200332
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38520.32091121456397065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36520.30424915773302724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28010.23335210591736286No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.14954196005771736No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG16430.13687879686620036No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA15630.130213974133823No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14940.12446556452714748No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG14540.12113315316095881No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC14370.11971687833032861No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12300.10247164951030215No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC12100.10080544382720781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA400.001931236423.12500224
ACCGTCG909.4791176E-820.5555558
GGTATCA15900.017.6855341
GCCGTCT953.6066558E-617.52631636
GCGAACT650.001579989217.07692323
CCGCTAT650.001579989217.07692325
TCGCCAT4150.016.93975813
TCTAGCG1005.878226E-616.65000228
CTTATAC6800.016.05147237
CCGAGTT700.002592583215.85714313
CTAGATA1059.34371E-615.8571423
TACCGTC1059.34371E-615.8571427
GTCTTAG1659.731593E-1015.696971
ATACCGT957.0600894E-515.5789486
GTAACAC957.0600894E-515.5789483
TACTCCG1557.206836E-915.5161295
GCGTTAT1201.9355757E-615.4166661
CGCAATA1201.9355757E-615.41666636
GTCCTAG855.3643965E-415.2352941
ATTACTC1954.1836756E-1115.1794873
Produced by FastQC (version 0.11.5)