##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630742.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200332
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.857189510901982	33.0	31.0	34.0	30.0	34.0
2	32.05379844909575	33.0	31.0	34.0	30.0	34.0
3	32.14382437525618	34.0	31.0	34.0	30.0	34.0
4	35.77822135875741	37.0	35.0	37.0	35.0	37.0
5	35.708943858865716	37.0	35.0	37.0	33.0	37.0
6	35.78419970474835	37.0	35.0	37.0	35.0	37.0
7	35.737810872325326	37.0	35.0	37.0	33.0	37.0
8	35.73470256562351	37.0	35.0	37.0	33.0	37.0
9	37.414311207232664	39.0	37.0	39.0	35.0	39.0
10	37.303892589716845	39.0	37.0	39.0	34.0	39.0
11	37.4006324916773	39.0	37.0	39.0	34.0	39.0
12	37.34366991798936	39.0	37.0	39.0	34.0	39.0
13	37.375595251980286	39.0	37.0	39.0	34.0	39.0
14	38.63963553416888	40.0	38.0	41.0	34.0	41.0
15	38.64960111035947	40.0	38.0	41.0	35.0	41.0
16	38.607427778314666	40.0	38.0	41.0	34.0	41.0
17	38.586847638819926	40.0	38.0	41.0	34.0	41.0
18	38.55106503867263	40.0	38.0	41.0	34.0	41.0
19	38.62842863474439	40.0	38.0	41.0	34.0	41.0
20	38.589303625996806	40.0	38.0	41.0	34.0	41.0
21	38.54290979495673	40.0	38.0	41.0	34.0	41.0
22	38.484966659224284	40.0	38.0	41.0	34.0	41.0
23	38.42355864877384	40.0	38.0	41.0	34.0	41.0
24	38.36745500411553	40.0	38.0	41.0	34.0	41.0
25	38.31712559525198	40.0	38.0	41.0	34.0	41.0
26	38.176609471379585	40.0	38.0	41.0	34.0	41.0
27	38.05629359210619	40.0	38.0	41.0	33.0	41.0
28	37.96221128821026	40.0	37.0	41.0	33.0	41.0
29	37.8846810715702	40.0	37.0	41.0	33.0	41.0
30	37.79515250780617	40.0	37.0	41.0	33.0	41.0
31	37.751676202917196	40.0	37.0	41.0	33.0	41.0
32	37.65183465907766	40.0	37.0	41.0	33.0	41.0
33	37.585682961047446	40.0	37.0	41.0	32.0	41.0
34	37.524228296837876	40.0	37.0	41.0	32.0	41.0
35	37.47276420190414	40.0	37.0	41.0	32.0	41.0
36	37.451450098806	40.0	37.0	41.0	32.0	41.0
37	37.32320224737823	40.0	36.0	41.0	32.0	41.0
38	37.179263737032755	39.0	36.0	41.0	31.0	41.0
39	37.13554000059983	39.0	36.0	41.0	31.0	41.0
40	36.97073476338213	39.0	36.0	41.0	31.0	41.0
41	36.91855669931319	39.0	36.0	41.0	31.0	41.0
42	36.79446603106474	39.0	35.0	41.0	30.0	41.0
43	35.973518993078585	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	0.0
12	2.0
13	1.0
14	1.0
15	4.0
16	4.0
17	12.0
18	48.0
19	84.0
20	169.0
21	336.0
22	631.0
23	1003.0
24	1814.0
25	2965.0
26	4472.0
27	6757.0
28	9640.0
29	13878.0
30	17977.0
31	24062.0
32	30772.0
33	40140.0
34	52681.0
35	71343.0
36	101726.0
37	160096.0
38	308118.0
39	351588.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98044207769184	19.61040778717888	12.611927366761863	24.79722276836742
2	18.344508019447954	21.683417587800708	35.45210825005082	24.519966142700518
3	20.247898081530778	23.78900170952703	30.216390132063463	25.74671007687873
4	14.868886274797305	15.839034533778987	34.273684280682346	35.018394910741364
5	14.036866466944145	37.63817010627059	34.470963033560714	13.854000393224542
6	34.606342245312135	34.78271011686766	15.903016832009811	14.707930805810394
7	28.26826244738956	31.04332801258319	21.731820862894597	18.95658867713266
8	26.37262024173312	34.38623647457536	20.415518373250066	18.825624910441444
9	26.097196442317628	14.228146879363376	20.10951969954979	39.5651369787692
10	16.442617542479915	27.628772706217948	33.55621611354192	22.37239363776022
11	34.80078844852924	22.016242172998805	22.22068561031448	20.962283768157477
12	20.95195329292229	26.09053161958525	29.4903410056551	23.467174081837356
13	29.358794066974802	20.27530716501768	26.0278822859009	24.338016482106617
14	22.48103024829797	21.551537407983794	25.66081717391522	30.30661516980302
15	25.28400475868343	28.298087529116945	23.213827507722865	23.204080204476764
16	24.84687569772363	27.045767337703236	24.353095643538623	23.754261321034516
17	23.349040098905967	27.22296831210032	25.829270568476055	23.598721020517658
18	22.342651866316984	25.769620405021275	27.802391338396376	24.08533639026536
19	23.772089721843624	26.211414841893742	27.57295481583429	22.443540620428347
20	24.201970788081965	25.228270178583923	27.653432550327743	22.91632648300637
21	23.838321397746622	26.28197865257279	26.332964546475473	23.546735403205112
22	23.984780877290614	25.773452678092397	26.481840024259956	23.759926420357033
23	23.274394084303342	26.27123162591683	26.745683694177945	23.708690595601883
24	24.30977429577817	26.297224434573103	26.497752288533505	22.895248981115223
25	24.02293698743348	25.651486422089885	26.811832059796792	23.513744530679844
26	23.800331908172073	26.16401129020971	26.604222831683234	23.431433969934982
27	24.028518776471845	25.718051339129506	26.301473259064995	23.951956625333658
28	23.082280569042567	26.101445266809513	27.14107430277623	23.675199861371688
29	23.060369964309874	26.700112968745316	26.976536491570663	23.262980575374144
30	22.817020624293946	27.338019814517985	26.82849411662773	23.01646544456034
31	23.60230336273631	26.354208668934927	26.753431550604333	23.29005641772443
32	22.663896322017575	26.605722416798017	26.791754281315505	23.9386269798689
33	22.899331185038807	26.07162018508213	27.188894405881044	23.840154223998027
34	23.324796806216945	25.942905796063087	27.0371863784353	23.695111019284663
35	23.482669794690135	26.044710963300155	27.149488641475862	23.323130600533855
36	23.049956178790534	26.238324063675716	27.112748806163626	23.59897095137012
37	23.81382817420514	25.10813674883282	27.017775082227253	24.06025999473479
38	22.961730587870687	25.576007304645714	27.972760869492774	23.489501237990822
39	22.975726715608683	25.351486088848752	28.01508249384337	23.6577047016992
40	22.568172805523805	24.91252420163755	28.800531852854043	23.718771139984604
41	21.88069634067908	25.683810812341918	28.882509172462285	23.552983674516717
42	22.695137678575595	24.835712119646896	28.616332814587963	23.852817387189546
43	21.69174861621618	24.96892526401029	28.733800315246118	24.605525804527414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	137.5
2	127.0
3	387.0
4	647.0
5	647.0
6	867.5
7	1088.0
8	1125.0
9	1162.0
10	1729.0
11	2296.0
12	2296.0
13	3852.5
14	5409.0
15	7996.5
16	10584.0
17	10448.0
18	10312.0
19	10312.0
20	11766.0
21	13220.0
22	13956.0
23	14692.0
24	16312.0
25	17932.0
26	17932.0
27	20275.0
28	22618.0
29	29064.0
30	35510.0
31	37746.0
32	39982.0
33	39982.0
34	43531.0
35	47080.0
36	50267.0
37	53454.0
38	60718.0
39	67982.0
40	67982.0
41	72367.0
42	76752.0
43	73594.5
44	70437.0
45	74120.5
46	77804.0
47	77804.0
48	80444.0
49	83084.0
50	85921.0
51	88758.0
52	93136.0
53	97514.0
54	97514.0
55	86821.5
56	76129.0
57	71565.0
58	67001.0
59	60723.0
60	54445.0
61	54445.0
62	50550.0
63	46655.0
64	41461.5
65	36268.0
66	31643.0
67	27018.0
68	27018.0
69	23166.0
70	19314.0
71	16642.5
72	13971.0
73	11249.5
74	8528.0
75	8528.0
76	6779.0
77	5030.0
78	4149.0
79	3268.0
80	2571.0
81	1874.0
82	1874.0
83	1414.5
84	955.0
85	774.0
86	593.0
87	521.5
88	450.0
89	450.0
90	325.5
91	201.0
92	116.0
93	31.0
94	19.0
95	7.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1200332.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.47929206516922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.94116074414984	50.54795662962982
2	7.320731759918847	8.415809579182714
3	1.8325112991066668	3.159943565222243
4	0.74879492489039	1.7216080873876474
5	0.42725882983053776	1.2279267533625957
6	0.2765603488932998	0.9537895840609848
7	0.1990704293106481	0.8009699143519755
8	0.15414503647574038	0.7088118057588194
9	0.10487164165194726	0.5425152947879438
>10	0.7506176149357687	8.538761200639582
>50	0.11567759673710486	4.745639997506832
>100	0.1185506433541705	13.407913613618055
>500	0.008010176680843058	3.117540834571752
>1k	0.0020389540642145967	2.1108131399190873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3852	0.32091121456397065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3652	0.30424915773302724	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2801	0.23335210591736286	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1795	0.14954196005771736	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1643	0.13687879686620036	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1563	0.130213974133823	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1494	0.12446556452714748	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1454	0.12113315316095881	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1437	0.11971687833032861	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1230	0.10247164951030215	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1210	0.10080544382720781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.499308524641516E-4	0.0
3	0.0	0.0	0.0	7.497925573924548E-4	0.0
4	0.0	0.0	0.0	0.0017495159672490611	0.0
5	0.0	0.0	0.0	0.0019161365355584955	0.0
6	0.0	0.0	0.0	0.0019161365355584955	0.0
7	0.0	0.0	0.0	0.00208275710386793	0.0
8	0.0	0.0	0.0	0.00208275710386793	0.0
9	0.0	0.0	0.0	0.0037489627869622738	0.0
10	0.0	0.0	0.0	0.005581789038366052	0.0
11	0.0	0.0	0.0	0.005915030174984921	0.0
12	0.0	8.33102841547172E-5	0.0	0.006331581595758507	0.0
13	0.0	8.33102841547172E-5	0.0	0.006581512448222658	0.0
14	0.0	8.33102841547172E-5	0.0	0.00683144330068681	0.0
15	0.0	8.33102841547172E-5	0.0	0.00833102841547172	0.0
16	0.0	8.33102841547172E-5	0.0	0.010580406087649083	0.0
17	0.0	8.33102841547172E-5	0.0	0.012246611770743428	0.0
18	0.0	8.33102841547172E-5	0.0	0.013329645464754751	0.0
19	0.0	8.33102841547172E-5	0.0	0.014912540863694378	0.0
20	0.0	8.33102841547172E-5	0.0	0.01607888484186042	0.0
21	0.0	8.33102841547172E-5	0.0	0.018911434503120803	0.0
22	0.0	1.666205683094344E-4	0.0	0.022993638426701945	0.0
23	0.0	2.499308524641516E-4	0.0	0.028991978885841582	0.0
24	0.0	2.499308524641516E-4	0.0	0.03965569525764538	0.0
25	0.0	2.499308524641516E-4	0.0	0.04323803747629822	0.0
26	0.0	2.499308524641516E-4	0.0	0.05031941162944919	0.0
27	0.0	2.499308524641516E-4	0.0	0.06139967942202657	0.0
28	0.0	2.499308524641516E-4	0.0	0.0872258675099889	0.0
29	0.0	3.332411366188688E-4	0.0	0.12913094043981166	0.0
30	0.0	3.332411366188688E-4	0.0	0.18828124218966086	0.0
31	0.0	3.332411366188688E-4	0.0	0.4230496229376539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	40	0.0019312364	23.125002	24
ACCGTCG	90	9.4791176E-8	20.555555	8
GGTATCA	1590	0.0	17.685534	1
GCCGTCT	95	3.6066558E-6	17.526316	36
GCGAACT	65	0.0015799892	17.076923	23
CCGCTAT	65	0.0015799892	17.076923	25
TCGCCAT	415	0.0	16.939758	13
TCTAGCG	100	5.878226E-6	16.650002	28
CTTATAC	680	0.0	16.051472	37
CCGAGTT	70	0.0025925832	15.857143	13
CTAGATA	105	9.34371E-6	15.857142	3
TACCGTC	105	9.34371E-6	15.857142	7
GTCTTAG	165	9.731593E-10	15.69697	1
ATACCGT	95	7.0600894E-5	15.578948	6
GTAACAC	95	7.0600894E-5	15.578948	3
TACTCCG	155	7.206836E-9	15.516129	5
GCGTTAT	120	1.9355757E-6	15.416666	1
CGCAATA	120	1.9355757E-6	15.416666	36
GTCCTAG	85	5.3643965E-4	15.235294	1
ATTACTC	195	4.1836756E-11	15.179487	3
>>END_MODULE