FastQCFastQC Report
Fri 10 Feb 2017
ERR1630741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230954
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47540.21309269487403146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45810.20533816474925076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38860.17418557262946702No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC34460.15446306826586295No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA30960.13877471252208695No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT29850.1337992625576323No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA27510.1233104761460792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.10923577985023447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT500.00703690718.51
GGTATCA21750.018.0321851
AAGACGG3450.016.0869565
GCTTTAT10450.015.9330141
CGCGGTT2100.015.85714210
CTTATAC11100.015.537
CGAATTA2150.015.48837215
CGAACTA1802.0190782E-1015.41666624
CCTTATA2201.8189894E-1215.1363642
TCTAGCG2850.014.92982628
CGCAATA2850.014.92982636
CTAGCGG2900.014.67241429
CGCAAGA3950.014.5189872
GCGGGTA3750.014.30666723
CGGGTAA3900.014.2307724
TCTTATA15500.014.08387237
CGGTCCA3550.014.07042210
CCTATAC2009.858923E-1013.8750013
TATACGT800.00630107113.8750015
TAGACAG2800.013.8755
Produced by FastQC (version 0.11.5)