##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630741.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2230954
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1919748233267	33.0	31.0	34.0	30.0	34.0
2	32.38174655326824	34.0	31.0	34.0	30.0	34.0
3	32.49930298876624	34.0	31.0	34.0	31.0	34.0
4	36.041735060427065	37.0	35.0	37.0	35.0	37.0
5	35.99319439127835	37.0	35.0	37.0	35.0	37.0
6	36.0628341059475	37.0	35.0	37.0	35.0	37.0
7	36.01769646527898	37.0	35.0	37.0	35.0	37.0
8	36.009957175271204	37.0	35.0	37.0	35.0	37.0
9	37.74632466648797	39.0	37.0	39.0	35.0	39.0
10	37.66735844844851	39.0	37.0	39.0	35.0	39.0
11	37.749707524225066	39.0	37.0	39.0	35.0	39.0
12	37.70428076957212	39.0	37.0	39.0	35.0	39.0
13	37.74073288826215	39.0	37.0	39.0	35.0	39.0
14	39.08889784370274	40.0	38.0	41.0	36.0	41.0
15	39.10303977580892	40.0	38.0	41.0	36.0	41.0
16	39.07822976179697	40.0	38.0	41.0	36.0	41.0
17	39.06432584445937	40.0	38.0	41.0	36.0	41.0
18	39.046568418712354	40.0	38.0	41.0	36.0	41.0
19	39.091584586683545	40.0	39.0	41.0	36.0	41.0
20	39.068209384864055	40.0	39.0	41.0	36.0	41.0
21	39.02394580972983	40.0	39.0	41.0	36.0	41.0
22	38.96478457198131	40.0	38.0	41.0	35.0	41.0
23	38.934891530708384	40.0	38.0	41.0	35.0	41.0
24	38.893099992200646	40.0	38.0	41.0	35.0	41.0
25	38.84536525629843	40.0	38.0	41.0	35.0	41.0
26	38.74187679351524	40.0	38.0	41.0	35.0	41.0
27	38.63190007503516	40.0	38.0	41.0	34.0	41.0
28	38.55144884206487	40.0	38.0	41.0	34.0	41.0
29	38.49916806890685	40.0	38.0	41.0	34.0	41.0
30	38.44098981870536	40.0	38.0	41.0	34.0	41.0
31	38.41017699154711	40.0	38.0	41.0	34.0	41.0
32	38.344194456721205	40.0	38.0	41.0	34.0	41.0
33	38.28376515158986	40.0	38.0	41.0	34.0	41.0
34	38.239184223430875	40.0	38.0	41.0	34.0	41.0
35	38.19587001793852	40.0	38.0	41.0	34.0	41.0
36	38.1779485368143	40.0	38.0	41.0	34.0	41.0
37	38.07470974300681	40.0	38.0	41.0	33.0	41.0
38	37.95478884817885	40.0	37.0	41.0	33.0	41.0
39	37.90988429165281	40.0	37.0	41.0	33.0	41.0
40	37.759151017905346	40.0	37.0	41.0	33.0	41.0
41	37.73152785758918	40.0	37.0	41.0	33.0	41.0
42	37.623045567053374	40.0	37.0	41.0	33.0	41.0
43	36.86518233903523	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	4.0
10	7.0
11	6.0
12	3.0
13	1.0
14	5.0
15	6.0
16	8.0
17	14.0
18	29.0
19	68.0
20	147.0
21	298.0
22	577.0
23	1090.0
24	1942.0
25	3263.0
26	5128.0
27	7754.0
28	11843.0
29	16907.0
30	23687.0
31	32025.0
32	42880.0
33	57813.0
34	79277.0
35	109844.0
36	158589.0
37	252843.0
38	496404.0
39	928487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20687293417973	18.683397326883476	11.61005560849753	25.499674130439264
2	18.90289983567568	20.381504952589786	34.099896277556596	26.615698934177935
3	20.583929565558055	22.808762529393256	29.020410102583917	27.586897802464776
4	14.330282022847623	14.63772897155208	35.023223248888144	36.00876575671215
5	14.671839939326405	36.5429766817245	34.52823321323523	14.25695016571386
6	35.75990361074231	34.70152230839363	15.120885504586825	14.417688576277236
7	29.752159838347183	29.84369018814373	21.364626971241897	19.03952300226719
8	28.338459690338752	32.30532767596284	19.858948234701387	19.49726439899702
9	27.029019872216104	13.618389263068625	19.53626116898869	39.81632969572658
10	17.22841439133214	26.336311730318062	33.05944452462937	23.375829353720427
11	36.226833901550634	21.4654806867376	21.432266196434348	20.87541921527741
12	22.853989817808884	25.008449300164866	28.58346698318298	23.554093898843277
13	30.94017178301301	19.474673166725985	24.651427147310073	24.933727902950935
14	23.0773471797267	20.23313793112722	25.104910276052305	31.58460461309377
15	25.857368641397354	27.604737704139126	22.06513446713827	24.472759187325245
16	25.44427182272696	26.134111236717565	23.698426771686016	24.72319016886946
17	24.329277968080024	25.868395314291554	25.057800384947427	24.744526332680998
18	24.064592994745745	24.812120733999894	26.63519731917377	24.488088952080588
19	25.15385794597289	24.772317134284258	26.443978674593914	23.629846245148936
20	25.746698497593407	24.439051634412902	25.518500157331797	24.295749710661894
21	25.470807555870717	24.606020563400232	25.607789313450656	24.315382567278395
22	25.46004982621784	24.768327809537983	25.932538277346822	23.839084086897355
23	25.035298800423494	24.657792137354694	25.527733875283847	24.779175186937966
24	24.571999243373014	25.11320269265973	25.902775225307202	24.412022838660054
25	24.934489908801346	25.093793955411	25.448664562335217	24.523051573452435
26	24.72883797693722	25.457629337045944	25.62540509575724	24.188127590259594
27	24.87106412772294	24.526771954957386	25.666912002667917	24.93525191465176
28	24.471773062107065	25.06591350606064	25.705998420406694	24.756315011425606
29	24.495126300228513	25.453640012299672	26.130077088097735	23.92115659937408
30	24.67572168677615	25.35076922249405	25.31432741329494	24.659181677434855
31	24.099510792244036	25.912636477489002	25.776192606391707	24.211660123875255
32	23.914119251226158	25.23906812959837	25.71290129693396	25.133911322241513
33	23.60514829082088	25.64714467443076	26.02868548611939	24.719021548628973
34	25.226382973382687	24.498757033986358	25.757545874993387	24.517314117637568
35	24.273024006770196	24.77446868021483	26.097490132024237	24.855017180990732
36	23.765169519407394	25.590083883396968	26.14778251815143	24.49696407904421
37	24.445640743825287	24.34586280129487	26.53636067798798	24.67213577689186
38	24.31645834024368	24.80983471644866	26.15701623610348	24.716690707204183
39	24.10376906023163	24.666219025582777	26.508704348005384	24.721307566180208
40	23.526616864354892	24.361192566050217	27.04600812029293	25.06618244930196
41	22.71082236567854	24.669491168352195	27.489181758117827	25.130504707851443
42	23.352655411093192	24.535198843185473	27.704201879554667	24.40794386616667
43	23.55978653078459	24.123401916848128	27.379766682773376	24.937044869593905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	168.0
2	192.0
3	559.0
4	926.0
5	926.0
6	1231.0
7	1536.0
8	1519.5
9	1503.0
10	2126.0
11	2749.0
12	2749.0
13	4648.5
14	6548.0
15	9149.0
16	11750.0
17	12111.0
18	12472.0
19	12472.0
20	15951.5
21	19431.0
22	20625.0
23	21819.0
24	25999.0
25	30179.0
26	30179.0
27	34993.5
28	39808.0
29	45589.0
30	51370.0
31	58576.5
32	65783.0
33	65783.0
34	74276.5
35	82770.0
36	90817.5
37	98865.0
38	106341.5
39	113818.0
40	113818.0
41	117744.0
42	121670.0
43	124803.5
44	127937.0
45	131096.0
46	134255.0
47	134255.0
48	139998.5
49	145742.0
50	145432.5
51	145123.0
52	147535.0
53	149947.0
54	149947.0
55	146201.5
56	142456.0
57	139558.5
58	136661.0
59	137405.5
60	138150.0
61	138150.0
62	127900.0
63	117650.0
64	108733.0
65	99816.0
66	86880.0
67	73944.0
68	73944.0
69	65228.0
70	56512.0
71	47664.5
72	38817.0
73	28905.5
74	18994.0
75	18994.0
76	13988.0
77	8982.0
78	7166.0
79	5350.0
80	4340.0
81	3330.0
82	3330.0
83	2565.0
84	1800.0
85	1447.0
86	1094.0
87	877.5
88	661.0
89	661.0
90	498.5
91	336.0
92	188.5
93	41.0
94	27.5
95	14.0
96	14.0
97	11.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2230954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.24227773338525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5075059857763	50.65659417898461
2	9.113948926589227	10.798621871137748
3	2.2988547393792724	4.085681728170474
4	0.9500031724766689	2.251214071658396
5	0.49156104281671126	1.4560597910730044
6	0.3055503699767048	1.0860899927819139
7	0.20820327694458102	0.8634105450426257
8	0.14525263220037554	0.6884077422655924
9	0.1112207197758757	0.5930071893602499
>10	0.7010281080481278	7.991100149364937
>50	0.08026222693269656	3.369416890826781
>100	0.07425056594769293	9.242666804779617
>500	0.009325537887783049	3.681265816723125
>1k	0.0030326952480595054	3.236463227830988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4754	0.21309269487403146	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4581	0.20533816474925076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3886	0.17418557262946702	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	3446	0.15446306826586295	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3096	0.13877471252208695	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2985	0.1337992625576323	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2751	0.1233104761460792	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2437	0.10923577985023447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.482387355364566E-5	0.0
2	0.0	0.0	0.0	5.827103561973936E-4	0.0
3	0.0	0.0	0.0	7.620058504119763E-4	0.0
4	0.0	0.0	0.0	0.0012550684595020786	0.0
5	8.964774710729132E-5	0.0	0.0	0.0015688355743775982	0.0
6	8.964774710729132E-5	0.0	0.0	0.0016136594479312437	0.0
7	8.964774710729132E-5	0.0	0.0	0.0017481310685921808	0.0
8	8.964774710729132E-5	0.0	0.0	0.0019274265628067633	0.0
9	8.964774710729132E-5	0.0	0.0	0.0041237963669354	0.0
10	1.3447162066093697E-4	0.0	0.0	0.00712699589502966	0.0
11	1.3447162066093697E-4	0.0	0.0	0.007978649492548928	0.0
12	1.3447162066093697E-4	0.0	0.0	0.008830303090068195	0.0
13	1.7929549421458264E-4	0.0	0.0	0.009188894078497361	0.0
14	1.7929549421458264E-4	0.0	0.0	0.009637132814033817	0.0
15	3.1376711487551964E-4	0.0	0.0	0.01084737739998225	0.0
16	3.1376711487551964E-4	0.0	0.0	0.012281741353698911	0.0
17	4.482387355364566E-4	0.0	0.0	0.0139402246751838	0.0
18	4.930626090901023E-4	0.0	0.0	0.015419412502454106	0.0
19	4.930626090901023E-4	0.0	0.0	0.016674480961956187	0.0
20	5.378864826437479E-4	0.0	0.0	0.018422612030548367	0.0
21	5.378864826437479E-4	0.0	0.0	0.02147063543219627	0.0
22	5.378864826437479E-4	0.0	0.0	0.02550478405202438	0.0
23	5.378864826437479E-4	0.0	0.0	0.031869774096642066	0.0
24	5.378864826437479E-4	0.0	0.0	0.04244820825530244	0.0
25	5.378864826437479E-4	0.0	0.0	0.04567552715116493	0.0
26	5.378864826437479E-4	0.0	0.0	0.05060615324206595	0.0
27	5.827103561973936E-4	0.0	0.0	0.058271035619739356	0.0
28	6.723581033046849E-4	4.482387355364566E-5	0.0	0.07906931294863094	0.0
29	6.723581033046849E-4	8.964774710729132E-5	0.0	0.11174591676923863	0.0
30	6.723581033046849E-4	8.964774710729132E-5	0.0	0.15612155158734783	0.0
31	7.171819768583306E-4	8.964774710729132E-5	0.0	0.3332654998713555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAT	50	0.007036907	18.5	1
GGTATCA	2175	0.0	18.032185	1
AAGACGG	345	0.0	16.086956	5
GCTTTAT	1045	0.0	15.933014	1
CGCGGTT	210	0.0	15.857142	10
CTTATAC	1110	0.0	15.5	37
CGAATTA	215	0.0	15.488372	15
CGAACTA	180	2.0190782E-10	15.416666	24
CCTTATA	220	1.8189894E-12	15.136364	2
TCTAGCG	285	0.0	14.929826	28
CGCAATA	285	0.0	14.929826	36
CTAGCGG	290	0.0	14.672414	29
CGCAAGA	395	0.0	14.518987	2
GCGGGTA	375	0.0	14.306667	23
CGGGTAA	390	0.0	14.23077	24
TCTTATA	1550	0.0	14.083872	37
CGGTCCA	355	0.0	14.070422	10
CCTATAC	200	9.858923E-10	13.875001	3
TATACGT	80	0.006301071	13.875001	5
TAGACAG	280	0.0	13.875	5
>>END_MODULE