FastQCFastQC Report
Fri 10 Feb 2017
ERR1630740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787236
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64040.35831865517480627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.32631392832284045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49800.2786425519629193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.1797188507841158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGA500.007036372618.52
TACCCTT1258.587449E-917.7600025
GACGGAC2200.017.659097
GGTATCA29700.017.191921
GCGACTT650.00158039817.07692313
CCGCTTA650.00158039817.07692325
GTATTAG3900.016.6025641
CGTATGC1403.4762706E-815.85714331
AAGACGG2100.015.8571425
CTAGCGG1557.215931E-915.51612929
CGCAATA1802.0190782E-1015.41666636
GTATAGA2055.456968E-1215.3414641
TCGTATG1455.3503754E-815.31034530
CGTAGAC855.366221E-415.2352933
GGGTATA750.004105590314.81
CGCGCTT1001.09390305E-414.812
CGCTTAT750.004105590314.826
ACGGACC2650.014.66037758
AGTCGGT1904.5656634E-1014.60526411
CTCGTAT1651.6465492E-814.57575729
Produced by FastQC (version 0.11.5)