##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1787236
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.117786906709576	33.0	31.0	34.0	30.0	34.0
2	32.2958204736252	34.0	31.0	34.0	30.0	34.0
3	32.396684041727	34.0	31.0	34.0	30.0	34.0
4	35.96186177986567	37.0	35.0	37.0	35.0	37.0
5	35.91386699909805	37.0	35.0	37.0	35.0	37.0
6	35.99129102144317	37.0	35.0	37.0	35.0	37.0
7	35.94365601409103	37.0	35.0	37.0	35.0	37.0
8	35.94081699339091	37.0	35.0	37.0	35.0	37.0
9	37.661076097392844	39.0	37.0	39.0	35.0	39.0
10	37.583114932778884	39.0	37.0	39.0	35.0	39.0
11	37.67008106372074	39.0	37.0	39.0	35.0	39.0
12	37.62533039844766	39.0	37.0	39.0	35.0	39.0
13	37.65488665179081	39.0	37.0	39.0	35.0	39.0
14	38.980439628566124	40.0	38.0	41.0	36.0	41.0
15	38.98916315472607	40.0	38.0	41.0	36.0	41.0
16	38.955735000861665	40.0	38.0	41.0	36.0	41.0
17	38.93959107806691	40.0	38.0	41.0	36.0	41.0
18	38.9214490979367	40.0	38.0	41.0	35.0	41.0
19	38.99131843807981	40.0	38.0	41.0	35.0	41.0
20	38.95629676215116	40.0	38.0	41.0	35.0	41.0
21	38.912876643039866	40.0	38.0	41.0	35.0	41.0
22	38.85417258828716	40.0	38.0	41.0	35.0	41.0
23	38.80730692533051	40.0	38.0	41.0	35.0	41.0
24	38.75742711091316	40.0	38.0	41.0	35.0	41.0
25	38.70953696098333	40.0	38.0	41.0	35.0	41.0
26	38.58350212283101	40.0	38.0	41.0	34.0	41.0
27	38.46264119567869	40.0	38.0	41.0	34.0	41.0
28	38.37782307428901	40.0	38.0	41.0	34.0	41.0
29	38.30684755678601	40.0	38.0	41.0	34.0	41.0
30	38.225072122540055	40.0	38.0	41.0	34.0	41.0
31	38.187349628140886	40.0	38.0	41.0	34.0	41.0
32	38.08848355785134	40.0	38.0	41.0	33.0	41.0
33	38.01611650615811	40.0	37.0	41.0	33.0	41.0
34	37.960425483819705	40.0	37.0	41.0	33.0	41.0
35	37.903355796324604	40.0	37.0	41.0	33.0	41.0
36	37.86733313339705	40.0	37.0	41.0	33.0	41.0
37	37.74155903305439	40.0	37.0	41.0	33.0	41.0
38	37.59576071654779	40.0	37.0	41.0	33.0	41.0
39	37.54661779418051	40.0	37.0	41.0	33.0	41.0
40	37.38211909339337	40.0	37.0	41.0	32.0	41.0
41	37.32447253748246	40.0	36.0	41.0	32.0	41.0
42	37.197747247705394	40.0	36.0	41.0	31.0	41.0
43	36.421563800192025	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	6.0
12	5.0
13	4.0
14	8.0
15	4.0
16	2.0
17	16.0
18	36.0
19	104.0
20	212.0
21	412.0
22	757.0
23	1309.0
24	2231.0
25	3436.0
26	5489.0
27	8292.0
28	11773.0
29	16716.0
30	22798.0
31	30131.0
32	39342.0
33	51309.0
34	68302.0
35	91849.0
36	130648.0
37	205694.0
38	387585.0
39	708761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41027150303597	19.172789715516025	12.237219930663885	25.179718850784116
2	18.43343576337988	21.402209892817737	35.10801035789341	25.056343985908967
3	20.43720023544736	23.576181321325222	29.669444885846076	26.31717355738134
4	14.706116036158626	15.676273306938759	34.08436266950755	35.533247987395065
5	14.22789155992829	37.268497277360126	34.30436719045498	14.1992439722566
6	34.70241199259639	34.43361704889561	15.876806420640587	14.987164537867411
7	28.515372340306484	30.53508322348028	21.751464272205798	19.19808016400744
8	26.671631502498833	33.794529653610375	20.488620417225256	19.045218426665535
9	26.39864013482271	14.131094046896997	20.20348739618047	39.266778422099826
10	16.81618991560152	27.2641106154979	33.24821120434011	22.671488264560473
11	34.68445129798191	22.115657920946084	21.998325906595436	21.201564874476567
12	21.22344223146803	25.91929661219895	29.372170211432625	23.485090944900396
13	29.551609300618388	20.304593237826456	25.82977290072492	24.314024560830244
14	22.736896526256185	21.46280625502172	25.650501668498173	30.149795550223924
15	25.360165081723956	27.921886085553332	23.19553769060158	23.52241114212113
16	24.77177048806089	26.854203921586183	24.51724338587629	23.856782204476634
17	23.24981144068271	26.995035910198766	26.034110772164393	23.721041876954136
18	22.7378477156906	25.657439756137414	27.611798329935162	23.992914198236832
19	23.751032320297934	26.039985765729877	27.565581713886694	22.643400200085495
20	24.0983283684975	25.174850998972715	27.686494676696306	23.040325955833477
21	24.02732487483466	25.922765656018566	26.580093507516633	23.469815961630136
22	24.067218878760276	25.609712427457822	26.682374347875715	23.640694345906194
23	23.456946928105747	25.95253229008368	26.854483683184537	23.736037098626035
24	23.981052306466523	26.195253452817646	26.728199297686484	23.095494943029347
25	24.04707604367862	25.58733149958931	26.8321587076357	23.533433749096368
26	23.727308536757317	26.05917741137712	26.816548010447416	23.396966041418146
27	23.944963060278553	25.775275341365102	26.50355073420634	23.776210864150006
28	23.219765045019237	25.99097153369784	27.116396491565748	23.672866929717173
29	23.073002110521497	26.490961462280303	27.139113133352282	23.296923293845918
30	22.94565463094969	26.90579196032309	27.068277496648456	23.08027591207876
31	23.51810281350644	26.4078163152488	26.748621894366497	23.325458976878263
32	22.692190622838844	26.382358009798367	27.02911087287857	23.89634049448422
33	22.911020145073174	25.995112005353516	27.215823763621593	23.878044085951718
34	23.429810053065182	25.88113713018314	27.018983503018067	23.670069313733606
35	23.36943750013988	25.99863700149281	27.256109433784907	23.375816064582406
36	22.98448554080155	26.20521296571913	27.104198885877413	23.706102607601906
37	23.557996817432056	25.247812823824052	27.2284689878673	23.965721370876594
38	22.89317135509804	25.482253043246665	27.93313250180726	23.691443099848033
39	22.877504705590084	25.3456734309291	27.960213424528153	23.816608438952663
40	22.446615891801642	25.03082972813887	28.758205407679792	23.764348972379697
41	21.887540313646323	25.37555196963356	28.930314742988617	23.806592973731505
42	22.422556394342998	25.05746303230239	28.616310324993457	23.903670248361156
43	21.73926666651746	25.012980938163736	28.768500634499304	24.4792517608195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	235.0
1	240.0
2	245.0
3	656.0
4	1067.0
5	1067.0
6	1545.5
7	2024.0
8	2069.5
9	2115.0
10	3042.5
11	3970.0
12	3970.0
13	6697.0
14	9424.0
15	13132.0
16	16840.0
17	16959.0
18	17078.0
19	17078.0
20	19415.5
21	21753.0
22	21837.0
23	21921.0
24	24427.0
25	26933.0
26	26933.0
27	30545.5
28	34158.0
29	41763.5
30	49369.0
31	54035.0
32	58701.0
33	58701.0
34	64081.0
35	69461.0
36	74378.5
37	79296.0
38	88549.0
39	97802.0
40	97802.0
41	103540.0
42	109278.0
43	106747.0
44	104216.0
45	108775.5
46	113335.0
47	113335.0
48	116519.5
49	119704.0
50	123310.5
51	126917.0
52	131450.5
53	135984.0
54	135984.0
55	125653.0
56	115322.0
57	109307.0
58	103292.0
59	94337.0
60	85382.0
61	85382.0
62	79373.5
63	73365.0
64	65122.5
65	56880.0
66	49712.0
67	42544.0
68	42544.0
69	36728.0
70	30912.0
71	26861.0
72	22810.0
73	18474.0
74	14138.0
75	14138.0
76	11335.0
77	8532.0
78	7020.0
79	5508.0
80	4266.0
81	3024.0
82	3024.0
83	2323.0
84	1622.0
85	1325.0
86	1028.0
87	854.0
88	680.0
89	680.0
90	494.0
91	308.0
92	177.0
93	46.0
94	30.5
95	15.0
96	15.0
97	8.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1787236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.34442419593863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77257040504418	50.90123802492954
2	8.810431868660578	10.45700012326419
3	2.2322502158770368	3.974148111674474
4	0.935240616341872	2.2200526344585256
5	0.5212031449058234	1.5465250261774228
6	0.318036585820718	1.1324218835263637
7	0.21252189461980897	0.882839262566976
8	0.15062480088638672	0.7150993662584422
9	0.11489127119356829	0.6136340700709567
>10	0.7344171908204288	8.34880058791409
>50	0.09328866312110708	3.9138538252213078
>100	0.09753031294986232	11.25814553897583
>500	0.006048025737236654	2.447643003249404
>1k	7.560032171545817E-4	0.902394857722217
>5k	1.8900080428864544E-4	0.6862036839903702
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6404	0.35831865517480627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5832	0.32631392832284045	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4980	0.2786425519629193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3212	0.1797188507841158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.595231967126893E-5	0.0	0.0013428556721104543	0.0
3	0.0	5.595231967126893E-5	0.0	0.002294045106522026	0.0
4	0.0	5.595231967126893E-5	0.0	0.0041964239753451696	0.0
5	0.0	1.1190463934253786E-4	0.0	0.00469999485238659	0.0
6	0.0	1.1190463934253786E-4	0.0	0.0048118994917291285	0.0
7	0.0	1.678569590138068E-4	0.0	0.005315470368770549	0.0
8	0.0	1.678569590138068E-4	0.0	0.005930945885154507	0.0
9	0.0	1.678569590138068E-4	0.0	0.010630940737541098	0.0
10	0.0	1.678569590138068E-4	0.0	0.016338077344010528	0.0
11	0.0	1.678569590138068E-4	0.0	0.017960694614477326	0.0
12	0.0	1.678569590138068E-4	0.0	0.019471407245601587	0.0
13	0.0	1.678569590138068E-4	0.0	0.020702358278369505	0.0
14	0.0	1.678569590138068E-4	0.0	0.021709500032452346	0.0
15	0.0	1.678569590138068E-4	0.0	0.02349997426193295	0.0
16	0.0	2.2380927868507572E-4	0.0	0.025961876327468783	0.0
17	0.0	2.2380927868507572E-4	0.0	0.029542824786429996	0.0
18	0.0	2.2380927868507572E-4	0.0	0.03144520365525314	0.0
19	0.0	2.2380927868507572E-4	0.0	0.033963058040460244	0.0
20	0.0	2.2380927868507572E-4	0.0	0.03642496010599607	0.0
21	0.0	2.2380927868507572E-4	0.0	0.03939043304857333	0.0
22	0.0	2.2380927868507572E-4	0.0	0.04688804388452336	0.0
23	0.0	2.2380927868507572E-4	0.0	0.05617612894995401	0.0
24	0.0	2.2380927868507572E-4	0.0	0.0675344498432216	0.0
25	0.0	2.2380927868507572E-4	0.0	0.07296182485133469	0.0
26	0.0	2.2380927868507572E-4	0.0	0.08258562383479294	0.0
27	0.0	2.2380927868507572E-4	0.0	0.0979725117443919	0.0
28	0.0	2.2380927868507572E-4	0.0	0.13059271411274168	0.0
29	0.0	3.357139180276136E-4	0.0	0.17658552088252474	0.0
30	0.0	3.357139180276136E-4	0.0	0.24372830448804747	0.0
31	0.0	3.357139180276136E-4	0.0	0.4924363654268379	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACGA	50	0.0070363726	18.5	2
TACCCTT	125	8.587449E-9	17.760002	5
GACGGAC	220	0.0	17.65909	7
GGTATCA	2970	0.0	17.19192	1
GCGACTT	65	0.001580398	17.076923	13
CCGCTTA	65	0.001580398	17.076923	25
GTATTAG	390	0.0	16.602564	1
CGTATGC	140	3.4762706E-8	15.857143	31
AAGACGG	210	0.0	15.857142	5
CTAGCGG	155	7.215931E-9	15.516129	29
CGCAATA	180	2.0190782E-10	15.416666	36
GTATAGA	205	5.456968E-12	15.341464	1
TCGTATG	145	5.3503754E-8	15.310345	30
CGTAGAC	85	5.366221E-4	15.235293	3
GGGTATA	75	0.0041055903	14.8	1
CGCGCTT	100	1.09390305E-4	14.8	12
CGCTTAT	75	0.0041055903	14.8	26
ACGGACC	265	0.0	14.6603775	8
AGTCGGT	190	4.5656634E-10	14.605264	11
CTCGTAT	165	1.6465492E-8	14.575757	29
>>END_MODULE