FastQCFastQC Report
Fri 10 Feb 2017
ERR1630739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070221
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201361.8814805540164132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178861.6712436029567723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142961.3357988677105008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100880.9426090499065146No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.2489205500546149No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20920.19547364516300839No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.1879051149248613No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.1788415663680679No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18600.1737958795426365No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.14959527050954896No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC15160.1416529856917403No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15100.14109235382224794No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14480.13529915783749336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.13174848933070832No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13340.12464715231713824No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC12990.12137679974509936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12960.12109648381035318No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12590.11763925394848354No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10930.10212843889252779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG400.001931100423.12500218
GCCGTCT802.7253918E-823.12500236
TCTATAC753.7402788E-722.1999993
TTCTATA1600.020.81252
ATACTGG2400.020.8124986
TCTAATA2400.020.8124982
TTAGGCA450.00382563920.5555554
GCTATGC450.00382563920.5555553
AATACTG2450.020.3877545
TCTATAG1101.7553248E-920.1818183
CAGTCGG4150.019.61445810
GCAGTCG4150.019.1686749
GGCAGTC4250.018.7176488
CCGTGTT500.00703457318.57
GCCGAGT1103.8508006E-818.512
CCGACCA500.00703457318.59
CGGTACA902.1519354E-618.527
CTCTAAT2600.018.51
GTAAACA2400.018.536
CTCTATG6750.018.3629651
Produced by FastQC (version 0.11.5)