##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630739.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070221
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.011510706667124	33.0	31.0	34.0	30.0	34.0
2	32.12024058582293	33.0	31.0	34.0	30.0	34.0
3	32.19666872543148	34.0	31.0	34.0	30.0	34.0
4	35.80638484948436	37.0	35.0	37.0	35.0	37.0
5	35.80152790872166	37.0	35.0	37.0	35.0	37.0
6	35.92488093580672	37.0	35.0	37.0	35.0	37.0
7	35.8580779110109	37.0	35.0	37.0	35.0	37.0
8	35.89354908939369	37.0	35.0	37.0	35.0	37.0
9	37.54079858272264	39.0	37.0	39.0	35.0	39.0
10	37.4721912576935	39.0	37.0	39.0	35.0	39.0
11	37.60049653295908	39.0	37.0	39.0	35.0	39.0
12	37.561560649622834	39.0	37.0	39.0	35.0	39.0
13	37.6063897082939	39.0	37.0	39.0	35.0	39.0
14	38.77440827642141	40.0	38.0	41.0	35.0	41.0
15	38.77202932852187	40.0	38.0	41.0	35.0	41.0
16	38.689198772963714	40.0	38.0	41.0	34.0	41.0
17	38.773579475641014	40.0	38.0	41.0	35.0	41.0
18	38.818921512472656	40.0	38.0	41.0	35.0	41.0
19	38.91872239472034	40.0	38.0	41.0	35.0	41.0
20	38.900431779978156	40.0	38.0	41.0	35.0	41.0
21	38.84602712897617	40.0	38.0	41.0	35.0	41.0
22	38.73175820694978	40.0	38.0	41.0	35.0	41.0
23	38.632239509409736	40.0	38.0	41.0	34.0	41.0
24	38.564064805306565	40.0	38.0	41.0	34.0	41.0
25	38.47816105271715	40.0	38.0	41.0	34.0	41.0
26	38.26949573966498	40.0	37.0	41.0	34.0	41.0
27	38.073302616936125	40.0	37.0	41.0	34.0	41.0
28	37.92829798705127	40.0	37.0	41.0	33.0	41.0
29	37.806705344036416	40.0	37.0	41.0	33.0	41.0
30	37.61145501723476	40.0	36.0	41.0	33.0	41.0
31	37.439795145114886	39.0	35.0	41.0	33.0	41.0
32	37.21672252740322	39.0	35.0	41.0	33.0	41.0
33	37.02144697216743	39.0	35.0	41.0	32.0	41.0
34	36.85363210028583	39.0	35.0	41.0	31.0	41.0
35	36.649309815449335	39.0	35.0	41.0	31.0	41.0
36	36.487541358280204	39.0	35.0	41.0	31.0	41.0
37	36.20944739451011	39.0	35.0	41.0	30.0	41.0
38	35.92243471208283	39.0	35.0	41.0	29.0	41.0
39	35.703674287834005	39.0	35.0	41.0	27.0	41.0
40	35.357507468083696	38.0	35.0	41.0	25.0	41.0
41	35.12673457164455	38.0	35.0	41.0	24.0	41.0
42	34.79686625472683	38.0	35.0	41.0	21.0	41.0
43	33.978900619591656	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	11.0
12	5.0
13	8.0
14	2.0
15	9.0
16	10.0
17	14.0
18	40.0
19	112.0
20	197.0
21	432.0
22	810.0
23	1262.0
24	2052.0
25	3523.0
26	5329.0
27	8052.0
28	11867.0
29	16137.0
30	20712.0
31	25918.0
32	31580.0
33	39518.0
34	50947.0
35	64824.0
36	90591.0
37	154171.0
38	224752.0
39	317326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.20985572138839	19.202575916563028	12.583382310756377	20.004186051292212
2	18.983649171526253	22.173364193003128	34.62957650802965	24.21341012744097
3	22.693163374667474	26.71840675897782	28.553354867826364	22.03507499852834
4	12.844169568715246	15.425131818568314	33.23472441673262	38.49597419598382
5	12.168234411397272	42.12681306010628	31.95293308578322	13.752019442713234
6	32.58747492340367	33.20463717306986	17.51049549579012	16.69739240773635
7	27.316974718305847	32.81378332138876	21.298030967435697	18.5712109928697
8	31.73755700925323	29.80104109338165	19.631926489949272	18.829475407415853
9	28.46458815515674	11.042485617456581	22.16009590542514	38.33283032196154
10	19.257424401128365	28.908141402570127	30.637597281309187	21.196836914992325
11	32.73314577082677	22.77239934555573	20.38793856595974	24.106516317657757
12	21.9498589543655	30.016417169911634	28.21164974337076	19.822074132352103
13	30.911559388201127	19.890844975009834	28.201838685654646	20.995756951134393
14	22.928815637144105	21.856980941319595	29.58342248937369	25.63078093216261
15	27.02040045934438	26.00238642299114	27.096926709530088	19.880286408134396
16	23.06458198820617	25.39578274020039	28.569052560172153	22.970582711421287
17	21.232063284125427	27.310247135871933	28.724067272086796	22.73362230791584
18	20.564911359429498	23.343309465988803	32.637931791657984	23.453847382923716
19	21.29999317897892	25.261044214232385	33.099331820250214	20.339630786538482
20	22.851822193733817	22.914426085827134	34.095200897758495	20.13855082268055
21	23.608862094838358	24.43654161149893	31.834452884030494	20.120143409632217
22	23.916929307124416	23.96701242079907	31.719990544009136	20.39606772806738
23	22.06133125774957	24.3568384473861	31.935179743249293	21.64665055161504
24	20.438769188793714	26.15310295723967	32.013574766333306	21.3945530876333
25	21.121058173965938	25.407088816235152	32.38695559141523	21.08489741838368
26	21.238043357400013	26.352781341423874	31.690277054926035	20.718898246250074
27	21.30587981360859	26.028922998147113	31.985262856923946	20.679934331320354
28	20.208910122301845	25.96557159689447	32.68362328902161	21.14189499178207
29	19.854684219427575	26.31820904280518	33.50158518661099	20.325521551156257
30	19.098578704772194	27.70829576321152	32.91133326668043	20.281792265335852
31	20.77982024273491	27.831354458565098	30.98332026749615	20.405505031203834
32	18.976547834512687	27.611213011144425	32.181016818021696	21.23122233632119
33	19.186878224217242	26.998068623209598	32.32949082479226	21.485562327780897
34	20.32318558503337	26.556757903274185	32.46759314197722	20.65246336971523
35	19.110258535386617	27.459562090446738	32.220821680755655	21.209357693410986
36	19.412719428977752	28.390865064318493	30.90950373801299	21.286911768690768
37	19.89149904552424	27.256052721821007	31.40566294251374	21.44678529014101
38	19.419820765991325	26.850435564243273	31.337359293080585	22.392384376684817
39	19.50410242370501	27.5042257627163	31.904905622296702	21.08676619128199
40	18.696231899766495	27.366590638755923	31.769419587169377	22.1677578743082
41	18.483565543939058	27.520390648286664	31.860709143251718	22.13533466452256
42	18.493937233524665	27.964784843504287	32.204096163315796	21.337181759655248
43	18.224647058878492	26.88332596725349	31.26952283687201	23.622504136996003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	255.5
2	353.0
3	1571.5
4	2790.0
5	2790.0
6	4181.5
7	5573.0
8	5724.0
9	5875.0
10	8218.5
11	10562.0
12	10562.0
13	17362.5
14	24163.0
15	33052.0
16	41941.0
17	37191.0
18	32441.0
19	32441.0
20	33501.0
21	34561.0
22	25095.0
23	15629.0
24	14930.5
25	14232.0
26	14232.0
27	15327.0
28	16422.0
29	18477.0
30	20532.0
31	22981.5
32	25431.0
33	25431.0
34	29408.0
35	33385.0
36	36144.5
37	38904.0
38	44657.0
39	50410.0
40	50410.0
41	54348.5
42	58287.0
43	61713.0
44	65139.0
45	66944.0
46	68749.0
47	68749.0
48	71230.0
49	73711.0
50	72155.0
51	70599.0
52	69618.5
53	68638.0
54	68638.0
55	64737.0
56	60836.0
57	56481.5
58	52127.0
59	49113.0
60	46099.0
61	46099.0
62	42729.0
63	39359.0
64	34311.5
65	29264.0
66	26950.0
67	24636.0
68	24636.0
69	20024.5
70	15413.0
71	13054.0
72	10695.0
73	7969.5
74	5244.0
75	5244.0
76	4097.0
77	2950.0
78	2359.0
79	1768.0
80	1419.5
81	1071.0
82	1071.0
83	928.0
84	785.0
85	761.5
86	738.0
87	562.0
88	386.0
89	386.0
90	350.0
91	314.0
92	176.0
93	38.0
94	23.0
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1070221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.93164040035627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71287162010557	34.651359800744935
2	10.700521432327452	9.187818764579747
3	3.1582620253949854	4.067681086930751
4	1.4122793596672376	2.425258784563171
5	0.7473165430732999	1.6041762546230132
6	0.5054090422507124	1.301882355419763
7	0.3496017196548748	1.0506282718098474
8	0.2558498182248256	0.8787241914019784
9	0.20177887300971276	0.7796428214797926
>10	1.5906103561369969	13.736947274812433
>50	0.1994842730195231	5.983168652740812
>100	0.14807919954080032	12.143443361927005
>500	0.01268625342013989	3.5614870022115666
>1k	0.004374570144875824	2.7676248165207236
>5k	0.0	0.0
>10k+	8.74914028975165E-4	5.860156560234529
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20136	1.8814805540164132	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17886	1.6712436029567723	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14296	1.3357988677105008	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10088	0.9426090499065146	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2664	0.2489205500546149	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2092	0.19547364516300839	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2011	0.1879051149248613	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1914	0.1788415663680679	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1860	0.1737958795426365	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1601	0.14959527050954896	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1516	0.1416529856917403	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1510	0.14109235382224794	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1448	0.13529915783749336	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1410	0.13174848933070832	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1334	0.12464715231713824	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1299	0.12137679974509936	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1296	0.12109648381035318	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1259	0.11763925394848354	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1093	0.10212843889252779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8687728983079197E-4	0.0
2	0.0	9.343864491539598E-5	0.0	0.0010278250940693558	0.0
3	0.0	9.343864491539598E-5	0.0	0.0016818956084771277	0.0
4	0.0	9.343864491539598E-5	0.0	0.0023359661228848994	0.0
5	0.0	9.343864491539598E-5	0.0	0.0024294047678002956	0.0
6	0.0	9.343864491539598E-5	0.0	0.0024294047678002956	0.0
7	0.0	9.343864491539598E-5	0.0	0.0026162820576310876	0.0
8	0.0	9.343864491539598E-5	0.0	0.0028965979923772753	0.0
9	0.0	9.343864491539598E-5	0.0	0.005232564115262175	0.0
10	0.0	9.343864491539598E-5	0.0	0.00859635533221643	0.0
11	0.0	9.343864491539598E-5	0.0	0.011212637389847518	0.0
12	0.0	1.8687728983079197E-4	0.0	0.014763305896632564	0.0
13	0.0	1.8687728983079197E-4	0.0	0.0162583242152789	0.0
14	0.0	1.8687728983079197E-4	0.0	0.019528676787317758	0.0
15	0.0	1.8687728983079197E-4	0.0	0.025882504641564687	0.0
16	0.0	1.8687728983079197E-4	0.0	0.046345567878036405	0.0
17	0.0	1.8687728983079197E-4	0.0	0.06933147452722381	0.0
18	0.0	1.8687728983079197E-4	0.0	0.079049093598425	0.0
19	0.0	1.8687728983079197E-4	0.0	0.0953074178137039	0.0
20	0.0	1.8687728983079197E-4	0.0	0.1045578436603281	0.0
21	0.0	1.8687728983079197E-4	0.0	0.13651386022139353	0.0
22	0.0	1.8687728983079197E-4	0.0	0.19837024315538565	0.0
23	0.0	1.8687728983079197E-4	0.0	0.3147013560750537	0.0
24	0.0	1.8687728983079197E-4	0.0	0.48018119622021993	0.0
25	0.0	1.8687728983079197E-4	0.0	0.5123240900711161	0.0
26	0.0	1.8687728983079197E-4	0.0	0.5635284674847532	0.0
27	0.0	1.8687728983079197E-4	0.0	0.6002498549365037	0.0
28	0.0	1.8687728983079197E-4	0.0	0.6539770757628565	0.0
29	0.0	1.8687728983079197E-4	0.0	0.7360162059985741	0.0
30	0.0	1.8687728983079197E-4	0.0	0.8900030928191467	0.0
31	0.0	1.8687728983079197E-4	0.0	1.4112038541572254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	40	0.0019311004	23.125002	18
GCCGTCT	80	2.7253918E-8	23.125002	36
TCTATAC	75	3.7402788E-7	22.199999	3
TTCTATA	160	0.0	20.8125	2
ATACTGG	240	0.0	20.812498	6
TCTAATA	240	0.0	20.812498	2
TTAGGCA	45	0.003825639	20.555555	4
GCTATGC	45	0.003825639	20.555555	3
AATACTG	245	0.0	20.387754	5
TCTATAG	110	1.7553248E-9	20.181818	3
CAGTCGG	415	0.0	19.614458	10
GCAGTCG	415	0.0	19.168674	9
GGCAGTC	425	0.0	18.717648	8
CCGTGTT	50	0.007034573	18.5	7
GCCGAGT	110	3.8508006E-8	18.5	12
CCGACCA	50	0.007034573	18.5	9
CGGTACA	90	2.1519354E-6	18.5	27
CTCTAAT	260	0.0	18.5	1
GTAAACA	240	0.0	18.5	36
CTCTATG	675	0.0	18.362965	1
>>END_MODULE