FastQCFastQC Report
Fri 10 Feb 2017
ERR1630738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839248
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58440.317738554017729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.2732094856158604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45590.24787304376571295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.1913825650483241No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG24310.1321735839864988No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT23210.1261928788287387No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG21160.11504701921654938No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA19220.10449923012013605No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC18830.10237879829147564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.017.3326971
GCGTATA803.3839472E-416.18759
TATACTG1751.3278623E-1015.8571435
TATACAG1659.749783E-1015.6969715
CGTATAT855.3663243E-415.23529410
ATCTCGC5350.015.21495411
TCTCGCC5650.014.73451312
TAGCGTA908.277344E-414.3888887
GTTAGAC800.006300518313.8753
CTAGTAC800.006300518313.8753
TACACCG800.006300518313.8755
TCTAATA2405.456968E-1213.8749992
TATACCC1203.3024764E-513.8749993
CGGCCTT6300.013.80158824
GTAGAAC9850.013.5228433
ACTGTCC2750.013.4545458
CTTATAC13100.013.41603137
AATACGA1254.847473E-513.325
TCTATAC1551.8890732E-613.1290323
TAGAACA10300.013.1116514
Produced by FastQC (version 0.11.5)