FastQCFastQC Report
Fri 10 Feb 2017
ERR1630737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452777
Sequences flagged as poor quality0
Sequence length43
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211864.679124602177231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191054.2195164507031055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168253.715957303484938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109082.409132972743757No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.6510931429820861No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.6424796312533543No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA28930.6389458828518233No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.5002462580917317No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA21870.4830192346342681No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.47528916000591903No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16560.36574295955845815No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA14830.3275343049669042No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13470.29749744355389074No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12980.28667533907420206No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.28358330922286246No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12010.2652519893899204No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10190.22505560132250532No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.22196357147116572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9620.21246662264205116No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8890.19634389556006598No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT8850.19546045845968324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8410.185742650355473No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8060.17801257572712395No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.17492054587578434No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA7720.1705033603738706No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA7390.1632150042957129No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.16233156719533015No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA7280.16078555226966035No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT6980.15415977401678974TruSeq Adapter, Index 6 (95% over 21bp)
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA6860.15150946271564147No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6810.15040516634016304No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA6690.14775485503901478No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA6430.14201251388652691No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA6160.13604931345894336No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6150.13582845418384767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT5960.1316321279570296No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5510.1216934605777237No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5390.11904314927657544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5220.11528854159994877No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5160.11396338594937463No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC5110.1128590895738962No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5090.11241737102370482No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA5080.11219651174860913No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA5060.11175479319841776No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA4950.10932534117236521No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.10645417059612126No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA4790.10579159277083419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGACT451.07751475E-732.8888931
AACTTAC250.005492869329.67
TGTGGAG250.005492869329.65
GCTGAGT250.005492869329.66
TATTGAC502.7146234E-729.630
GGTGCAT250.005492869329.66
GCGTTAT604.310641E-827.7500021
GCCGTCT1050.026.4285736
TGAACCA509.0752055E-625.9000025
CCGTCTT1000.025.90000237
GAACCAT451.3214821E-424.6666686
TACACTG551.8993585E-523.5454545
CCCGACA400.001929389823.12513
TATACCA652.6768867E-622.7692285
ATGCCCC903.812602E-922.61111337
GATACAC753.7304198E-722.23
ATACACT603.719868E-521.5833344
TGGGCAG5850.021.1880346
GTTATAC705.09066E-621.1428573
AGTCGGT5000.020.7211
Produced by FastQC (version 0.11.5)