##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630737.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452777
Sequences flagged as poor quality	0
Sequence length	43
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00181767183404	33.0	31.0	34.0	30.0	34.0
2	31.995629194945856	33.0	31.0	34.0	30.0	34.0
3	31.986465743622137	33.0	31.0	34.0	30.0	34.0
4	35.679524357465155	37.0	35.0	37.0	33.0	37.0
5	35.702003414484395	37.0	35.0	37.0	35.0	37.0
6	35.8866815231339	37.0	35.0	37.0	35.0	37.0
7	35.76438069954967	37.0	35.0	37.0	35.0	37.0
8	35.897229762112474	37.0	35.0	37.0	35.0	37.0
9	37.42342256784245	39.0	37.0	39.0	34.0	39.0
10	37.39196116410507	39.0	37.0	39.0	34.0	39.0
11	37.55421322196136	39.0	37.0	39.0	35.0	39.0
12	37.57543338111255	39.0	37.0	39.0	35.0	39.0
13	37.617317134041706	39.0	37.0	39.0	35.0	39.0
14	38.6349748330856	40.0	38.0	41.0	34.0	41.0
15	38.51838763894809	40.0	38.0	41.0	34.0	41.0
16	38.40731309231697	40.0	38.0	41.0	34.0	41.0
17	38.672918456547045	40.0	38.0	41.0	35.0	41.0
18	38.8224004311173	40.0	38.0	41.0	35.0	41.0
19	38.99774281820852	40.0	39.0	41.0	35.0	41.0
20	39.03287048591249	40.0	39.0	41.0	35.0	41.0
21	38.992972257866455	40.0	38.0	41.0	35.0	41.0
22	38.82940167013784	40.0	38.0	41.0	35.0	41.0
23	38.67918865136701	40.0	38.0	41.0	35.0	41.0
24	38.55760341183408	40.0	38.0	41.0	35.0	41.0
25	38.43754430989207	40.0	37.0	41.0	35.0	41.0
26	38.04410118005111	40.0	37.0	41.0	34.0	41.0
27	37.72353719380622	40.0	35.0	41.0	33.0	41.0
28	37.476592229728986	39.0	35.0	41.0	33.0	41.0
29	37.167932558411756	39.0	35.0	41.0	33.0	41.0
30	36.76314167901638	38.0	35.0	41.0	33.0	41.0
31	36.2767455060659	37.0	35.0	40.0	32.0	41.0
32	35.814184466083745	37.0	35.0	40.0	31.0	41.0
33	35.38137537904973	35.0	35.0	40.0	30.0	41.0
34	35.01582677565336	35.0	35.0	40.0	29.0	41.0
35	34.59402531489011	35.0	35.0	40.0	25.0	41.0
36	34.099684833814436	35.0	34.0	40.0	21.0	41.0
37	33.499380489733355	35.0	33.0	40.0	18.0	41.0
38	32.87878580404924	35.0	33.0	40.0	15.0	41.0
39	32.29786848713605	35.0	33.0	40.0	10.0	41.0
40	31.55898599089618	35.0	32.0	39.0	8.0	41.0
41	30.865894248161897	35.0	30.0	39.0	7.0	41.0
42	30.086086528246796	35.0	25.0	39.0	7.0	41.0
43	29.186807192061433	35.0	21.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	9.0
12	9.0
13	10.0
14	10.0
15	7.0
16	12.0
17	16.0
18	23.0
19	54.0
20	163.0
21	289.0
22	516.0
23	906.0
24	1521.0
25	2486.0
26	4021.0
27	6004.0
28	8454.0
29	11242.0
30	13817.0
31	15727.0
32	18662.0
33	22778.0
34	27607.0
35	32953.0
36	44888.0
37	89044.0
38	78975.0
39	72567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.47427762452598	18.488792496085267	14.218478412110155	12.818451467278594
2	15.453523478445238	28.285668220779765	33.63753017489846	22.623278125876535
3	24.77179715400738	33.74928496809687	29.213718894731844	12.265198983163897
4	12.466401782776067	17.453404214436688	28.854159994876067	41.226034007911174
5	9.269684635041092	48.79201019486414	27.362918169429985	14.575387000664787
6	28.21587668984953	29.510553760460446	20.676624475183147	21.596945074506877
7	21.62433162461874	39.04615296271675	23.101217597183602	16.22829781548091
8	36.63724084924809	26.86929768959112	20.153408852481462	16.34005260867933
9	30.560518754265342	9.763746833430144	25.027773053843283	34.647961358461224
10	20.037678592331325	34.99802330948789	24.506324305342364	20.457973792838416
11	24.025071944908863	29.207534835029165	18.95701415928813	27.810379060773847
12	20.3102189377994	36.381706667962376	29.624958864960014	13.683115529278208
13	30.22437093756971	21.450957093668627	32.58822775891885	15.736444209842816
14	23.985538134666733	24.50146540129026	35.835963399200935	15.677033064842075
15	26.77587421622565	24.696925859749943	34.44167879552186	14.08552112850255
16	16.470359580985782	26.627015064811154	37.4506655594255	19.45195979477756
17	14.547117013452537	31.767956411213465	36.12595162740157	17.558974947932427
18	12.892218465160553	23.85810233293652	42.696294202223164	20.553384999679754
19	14.091042610379944	28.732687393573435	45.60125624755674	11.575013748489875
20	16.05315641033003	24.05069162081996	47.0783630793967	12.817788889453308
21	19.473604003736938	24.755453567650303	43.17202508077928	12.59891734783348
22	17.80920850661584	25.983210277907226	42.09776556671386	14.109815648763076
23	15.027927655335848	28.2379626173591	42.11565516799661	14.618454559308445
24	12.85710184042034	30.405696402423267	43.47791517678681	13.259286580369587
25	12.979016160273158	29.355289690068183	43.11106792085287	14.554626228805793
26	14.374846778877902	29.32326509517931	42.89241723850814	13.409470887434653
27	12.522279179375278	31.46736693780824	42.73384027898944	13.27651360382705
28	12.39616853329564	31.066949072059757	44.48392917484767	12.052953219796942
29	12.043897989518019	32.32297576952893	44.13342550527081	11.499700735682246
30	11.346866117316031	33.363885533054905	43.931780987108446	11.357467362520621
31	10.783674965822026	34.309384089739545	42.68480951991819	12.222131424520239
32	9.69218842829914	35.2281586741376	43.404369038179944	11.675283859383317
33	10.104753554177885	34.9361827124611	43.15700665007277	11.80205708328824
34	10.780362076695592	35.90663836723155	40.70414354086007	12.608856015212789
35	10.454152927379262	36.166810593294265	40.52591010585785	12.853126373468617
36	10.244557475313453	36.2421236061019	39.470644489450656	14.042674429133989
37	10.447527149126392	36.71630846973234	39.31560127833348	13.520563102807785
38	10.735748503126263	35.49628183410377	39.42779779008209	14.340171872687879
39	11.16200690406094	36.097460780914226	39.57886553424754	13.161666780777292
40	10.496337048922538	36.33775567221833	39.49206783913494	13.673839439724192
41	9.926078400625475	37.50764725240019	38.33542781545882	14.230846531515514
42	10.21915865867745	36.83402646335834	38.34072843806112	14.606086439903088
43	9.713611777983422	37.70509544433573	36.956824220311546	15.624468557369301
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36.0
1	143.0
2	250.0
3	1457.0
4	2664.0
5	2664.0
6	4308.5
7	5953.0
8	6052.0
9	6151.0
10	8557.0
11	10963.0
12	10963.0
13	19001.0
14	27039.0
15	38399.0
16	49759.0
17	43988.5
18	38218.0
19	38218.0
20	38365.5
21	38513.0
22	27006.5
23	15500.0
24	14508.5
25	13517.0
26	13517.0
27	13746.0
28	13975.0
29	14024.5
30	14074.0
31	13616.0
32	13158.0
33	13158.0
34	12536.5
35	11915.0
36	11543.5
37	11172.0
38	12434.5
39	13697.0
40	13697.0
41	15289.0
42	16881.0
43	17894.0
44	18907.0
45	22063.5
46	25220.0
47	25220.0
48	26181.0
49	27142.0
50	23710.0
51	20278.0
52	15349.0
53	10420.0
54	10420.0
55	9711.0
56	9002.0
57	8222.5
58	7443.0
59	6810.0
60	6177.0
61	6177.0
62	5868.0
63	5559.0
64	5243.5
65	4928.0
66	4489.5
67	4051.0
68	4051.0
69	3721.5
70	3392.0
71	3035.5
72	2679.0
73	2229.0
74	1779.0
75	1779.0
76	1457.0
77	1135.0
78	889.0
79	643.0
80	493.5
81	344.0
82	344.0
83	241.5
84	139.0
85	100.0
86	61.0
87	48.0
88	35.0
89	35.0
90	21.5
91	8.0
92	4.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	452777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.064839307849056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42381020143883	25.930706822198278
2	10.45756566867021	6.91555456771719
3	3.621322039757409	3.5921529397965264
4	1.8696495185931972	2.472786435771256
5	1.0977922318235382	1.8149161869325132
6	0.6974798051977629	1.3837234607600333
7	0.5436599859677413	1.2583221051891584
8	0.4078650442881698	1.0788793718941656
9	0.3374179176886763	1.0041002305168605
>10	2.1284616108931655	13.642897562413655
>50	0.22087430963684632	5.144311190747829
>100	0.16465175809292182	11.15526564340009
>500	0.017402218335024255	3.8762146915873616
>1k	0.009370425257320753	5.675893702263264
>5k	0.0	0.0
>10k+	0.002677264359234501	15.054275088811803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21186	4.679124602177231	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19105	4.2195164507031055	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16825	3.715957303484938	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10908	2.409132972743757	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2948	0.6510931429820861	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2909	0.6424796312533543	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2893	0.6389458828518233	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2265	0.5002462580917317	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	2187	0.4830192346342681	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2152	0.47528916000591903	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1656	0.36574295955845815	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1483	0.3275343049669042	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1347	0.29749744355389074	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1298	0.28667533907420206	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1284	0.28358330922286246	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.2652519893899204	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1019	0.22505560132250532	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1005	0.22196357147116572	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	962	0.21246662264205116	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	889	0.19634389556006598	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	885	0.19546045845968324	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	841	0.185742650355473	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	806	0.17801257572712395	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	792	0.17492054587578434	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	772	0.1705033603738706	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	739	0.1632150042957129	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	735	0.16233156719533015	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	728	0.16078555226966035	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	698	0.15415977401678974	TruSeq Adapter, Index 6 (95% over 21bp)
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	686	0.15150946271564147	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	681	0.15040516634016304	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	669	0.14775485503901478	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	643	0.14201251388652691	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	616	0.13604931345894336	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	615	0.13582845418384767	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	596	0.1316321279570296	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	551	0.1216934605777237	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	539	0.11904314927657544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	522	0.11528854159994877	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	516	0.11396338594937463	No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC	511	0.1128590895738962	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	509	0.11241737102370482	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	508	0.11219651174860913	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	506	0.11175479319841776	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	495	0.10932534117236521	No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	482	0.10645417059612126	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	479	0.10579159277083419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0035337484015309966	0.0
3	0.0	0.0	0.0	0.006625778252870618	0.0
4	0.0	0.0	0.0	0.009276089554018865	0.0
5	0.0	0.0	0.0	0.009938667379305927	0.0
6	0.0	0.0	0.0	0.010159526654401615	0.0
7	0.0	0.0	0.0	0.011042963754784365	0.0
8	0.0	0.0	0.0	0.01126382302988005	0.0
9	0.0	0.0	0.0	0.018110460557846358	0.0
10	0.0	2.2085927509568729E-4	0.0	0.027828268662056597	0.0
11	0.0	2.2085927509568729E-4	0.0	0.035337484015309964	0.0
12	0.0	4.4171855019137457E-4	0.0	0.04328841791875471	0.0
13	0.0	4.4171855019137457E-4	0.0	0.04704302559538139	0.0
14	0.0	4.4171855019137457E-4	0.0	0.058748567175452814	0.0
15	0.0	4.4171855019137457E-4	0.0	0.07619644990801211	0.0
16	0.0	4.4171855019137457E-4	0.0	0.11595111942523582	0.0
17	0.0	6.625778252870618E-4	0.0	0.17381624950030589	0.0
18	0.0	6.625778252870618E-4	0.0	0.20252795526274522	0.0
19	0.0	6.625778252870618E-4	0.0	0.24802496593245682	0.0
20	0.0	6.625778252870618E-4	0.0	0.2793869829960444	0.0
21	0.0	6.625778252870618E-4	0.0	0.35712944782972633	0.0
22	0.0	6.625778252870618E-4	0.0	0.48103150115840687	0.0
23	0.0	6.625778252870618E-4	0.0	0.706970539581295	0.0
24	0.0	6.625778252870618E-4	0.0	1.0150692283397786	0.0
25	0.0	6.625778252870618E-4	0.0	1.0881736483964513	0.0
26	0.0	6.625778252870618E-4	0.0	1.1981615673941035	0.0
27	0.0	6.625778252870618E-4	0.0	1.2739162987519241	0.0
28	0.0	6.625778252870618E-4	0.0	1.3571802454629984	0.0
29	0.0	6.625778252870618E-4	0.0	1.4819657358920617	0.0
30	0.0	6.625778252870618E-4	0.0	1.7313158574750926	0.0
31	0.0	6.625778252870618E-4	0.0	2.3810838448066045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGACT	45	1.07751475E-7	32.88889	31
AACTTAC	25	0.0054928693	29.6	7
TGTGGAG	25	0.0054928693	29.6	5
GCTGAGT	25	0.0054928693	29.6	6
TATTGAC	50	2.7146234E-7	29.6	30
GGTGCAT	25	0.0054928693	29.6	6
GCGTTAT	60	4.310641E-8	27.750002	1
GCCGTCT	105	0.0	26.42857	36
TGAACCA	50	9.0752055E-6	25.900002	5
CCGTCTT	100	0.0	25.900002	37
GAACCAT	45	1.3214821E-4	24.666668	6
TACACTG	55	1.8993585E-5	23.545454	5
CCCGACA	40	0.0019293898	23.125	13
TATACCA	65	2.6768867E-6	22.769228	5
ATGCCCC	90	3.812602E-9	22.611113	37
GATACAC	75	3.7304198E-7	22.2	3
ATACACT	60	3.719868E-5	21.583334	4
TGGGCAG	585	0.0	21.188034	6
GTTATAC	70	5.09066E-6	21.142857	3
AGTCGGT	500	0.0	20.72	11
>>END_MODULE