FastQCFastQC Report
Fri 10 Feb 2017
ERR1630736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758004
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77200.4391343819467987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68220.38805372456490433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53820.3061426481395947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43040.24482310620453648No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG31520.17929424506428882No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT30760.17497116047517525No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG26760.15221808369036702No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC22400.12741722999492605No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA21190.12053442426752158No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19900.11319655700442093No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18660.10614310320113038No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18220.10364026475480147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT2300.020.91304211
TACACCG555.144585E-420.1818185
CGGTGAT2800.018.514
CAGTCGG2500.018.510
TGTACCG1501.2732926E-1118.55
GCGTATA701.21966375E-418.59
CCGCTTA953.6085858E-617.52631625
GCAGTCG2750.017.4909089
AACCACG752.0680515E-417.2666667
CGGGTAA1901.8189894E-1216.55263124
GCGGGTA1901.8189894E-1216.55263123
GTATTAG3400.016.323531
AGCGTAT803.3838407E-416.18758
GTCGGTG3000.016.03333312
ACGAACG1059.348654E-615.85714215
GGTATCA36000.015.2624991
CTTATAC14100.015.21985837
TCGGTGA3050.015.16393413
CGAATTA1353.9753286E-715.07407315
CGCGGGT2109.094947E-1214.9761922
Produced by FastQC (version 0.11.5)