##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630736.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1758004
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14166634433141	33.0	31.0	34.0	30.0	34.0
2	32.31432408572449	34.0	31.0	34.0	30.0	34.0
3	32.412614533300264	34.0	31.0	34.0	30.0	34.0
4	35.9834021993124	37.0	35.0	37.0	35.0	37.0
5	35.93953654257897	37.0	35.0	37.0	35.0	37.0
6	36.01906252773031	37.0	35.0	37.0	35.0	37.0
7	35.96653534349182	37.0	35.0	37.0	35.0	37.0
8	35.969793015260485	37.0	35.0	37.0	35.0	37.0
9	37.69529136452477	39.0	37.0	39.0	35.0	39.0
10	37.61273296306493	39.0	37.0	39.0	35.0	39.0
11	37.70771738858387	39.0	37.0	39.0	35.0	39.0
12	37.665086655092935	39.0	37.0	39.0	35.0	39.0
13	37.688999001139926	39.0	37.0	39.0	35.0	39.0
14	39.017094955415345	40.0	38.0	41.0	36.0	41.0
15	39.03917738526192	40.0	38.0	41.0	36.0	41.0
16	38.997027879344984	40.0	38.0	41.0	36.0	41.0
17	38.99025201307847	40.0	38.0	41.0	36.0	41.0
18	38.978025078441235	40.0	38.0	41.0	36.0	41.0
19	39.05846403079857	40.0	39.0	41.0	36.0	41.0
20	39.025571614171525	40.0	39.0	41.0	36.0	41.0
21	38.983424383562266	40.0	39.0	41.0	35.0	41.0
22	38.924675370476976	40.0	38.0	41.0	35.0	41.0
23	38.87334784221196	40.0	38.0	41.0	35.0	41.0
24	38.831714831138044	40.0	38.0	41.0	35.0	41.0
25	38.783137012202474	40.0	38.0	41.0	35.0	41.0
26	38.66128916657755	40.0	38.0	41.0	35.0	41.0
27	38.535971476742944	40.0	38.0	41.0	34.0	41.0
28	38.461000088737	40.0	38.0	41.0	34.0	41.0
29	38.401252215580854	40.0	38.0	41.0	34.0	41.0
30	38.316795069863325	40.0	38.0	41.0	34.0	41.0
31	38.265214982446004	40.0	38.0	41.0	34.0	41.0
32	38.16419985392525	40.0	38.0	41.0	34.0	41.0
33	38.09358056068132	40.0	38.0	41.0	33.0	41.0
34	38.031218927829514	40.0	38.0	41.0	33.0	41.0
35	37.96465537052248	40.0	37.0	41.0	33.0	41.0
36	37.91897401826162	40.0	37.0	41.0	33.0	41.0
37	37.79003858921823	40.0	37.0	41.0	33.0	41.0
38	37.64126077073772	40.0	37.0	41.0	33.0	41.0
39	37.58404986564308	40.0	37.0	41.0	33.0	41.0
40	37.4140218110994	40.0	37.0	41.0	32.0	41.0
41	37.3499292379312	40.0	37.0	41.0	32.0	41.0
42	37.22593748364623	40.0	37.0	41.0	32.0	41.0
43	36.47603987249176	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	3.0
10	4.0
11	5.0
12	9.0
13	5.0
14	4.0
15	5.0
16	4.0
17	22.0
18	33.0
19	88.0
20	209.0
21	349.0
22	630.0
23	1153.0
24	1973.0
25	3199.0
26	5161.0
27	7768.0
28	11461.0
29	16259.0
30	22205.0
31	29135.0
32	37727.0
33	48945.0
34	64956.0
35	87996.0
36	125472.0
37	198762.0
38	373726.0
39	720733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62987569994152	19.2012646160077	11.90770896994546	24.261150714105316
2	17.575443514349228	21.161044002175196	37.174204381787526	24.08930810168805
3	19.65114982673532	24.48902277810517	30.465857870630558	25.393969524528952
4	13.756908402938786	15.437735067724534	35.077906534911186	35.7274499944255
5	12.985180921090054	38.87203897146992	35.152252213305545	12.990527894134484
6	34.06277801415696	35.26556253569389	16.498028445896594	14.173631004252549
7	27.824908248217866	31.589063506112613	22.29682071258086	18.28920753308866
8	26.221100748348697	35.30117110086211	20.468440344845632	18.009287805943558
9	26.117460483593895	13.632449072925887	20.31354877463305	39.93654166884717
10	15.48580094243244	28.317853656760732	34.484904471207116	21.711440929599704
11	33.77438276590952	22.199835722785615	23.103075988450538	20.92270552285433
12	20.385164083813233	26.64129319387214	30.546176231680928	22.427366490633695
13	28.982186616185174	20.67640346665878	27.141405821602227	23.200004095553822
14	21.983681493329936	21.84841445184425	26.945729361252873	29.22217469357294
15	25.006825923035443	28.840036768972084	24.12889845529362	22.024238852698858
16	24.19818157410336	27.678776612567436	25.2656990541546	22.85734275917461
17	22.67378231221317	27.858867215319194	26.705513753097264	22.761836719370375
18	21.442556444695235	26.343796714910773	28.93201608187467	23.28163075851932
19	22.818321232488664	26.91285116529883	28.749422640676585	21.519404961535923
20	23.431573534531207	25.717347628333044	29.094245519350352	21.7568333177854
21	23.049264961854465	26.94561559586895	27.337594226179235	22.66752521609735
22	23.08424781741111	26.302898059390078	27.65261057426491	22.960243548933903
23	22.411837515728063	26.88941549621047	27.853349594198875	22.845397393862584
24	23.457682690141773	26.879233494349275	27.64999397043465	22.013089845074298
25	23.1386276709268	26.209382913804518	27.962621245457917	22.689368169810763
26	22.899834130070236	26.74516098939479	27.778662619652746	22.576342260882228
27	23.048070425323264	26.429917110541272	27.42820835447473	23.093804109660727
28	22.162805090318336	26.706480758860618	28.24089137453612	22.889822776284923
29	22.193749274745677	27.365978689468285	28.08338320049329	22.35688883529275
30	21.825718257751404	28.03389525848633	27.93116511680292	22.209221366959348
31	22.581063524315077	27.181166823283682	27.88315612478697	22.354613527614273
32	21.678164554801924	27.19771968664463	28.025874798919688	23.098240959633767
33	22.04232754874278	26.793852573714283	28.179970011444798	22.983849866098144
34	22.33737807194978	26.8405532638151	28.019674585495824	22.802394078739297
35	22.59699067806444	26.81268074475371	28.015237735522785	22.575090841659062
36	22.20171285162036	27.144705017736026	27.86995934025179	22.78362279039183
37	22.944828339412197	25.906141282954987	27.755682012100085	23.39334836553273
38	22.212804976552956	26.245389657816474	28.770639884778422	22.771165480852147
39	22.250973262859468	26.140270443070662	28.715065494731522	22.89369079933834
40	21.7355591909916	25.62417377889925	29.52319789943595	23.117069130673194
41	21.05000898746533	26.34897303987932	29.72109278477182	22.879925187883533
42	21.93982493782722	25.479009149012178	29.32416536026084	23.257000552899765
43	20.96747220142844	25.747950516608608	29.311651168029197	23.972926113933756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	217.5
2	255.0
3	987.0
4	1719.0
5	1719.0
6	2287.0
7	2855.0
8	2889.5
9	2924.0
10	4306.5
11	5689.0
12	5689.0
13	8808.5
14	11928.0
15	16789.0
16	21650.0
17	21071.0
18	20492.0
19	20492.0
20	22715.0
21	24938.0
22	25570.0
23	26202.0
24	28778.0
25	31354.0
26	31354.0
27	34475.5
28	37597.0
29	47409.0
30	57221.0
31	60587.0
32	63953.0
33	63953.0
34	68811.0
35	73669.0
36	77812.0
37	81955.0
38	92792.5
39	103630.0
40	103630.0
41	109386.5
42	115143.0
43	109906.0
44	104669.0
45	110126.5
46	115584.0
47	115584.0
48	119038.0
49	122492.0
50	124758.0
51	127024.0
52	131691.5
53	136359.0
54	136359.0
55	119219.5
56	102080.0
57	95324.5
58	88569.0
59	79685.0
60	70801.0
61	70801.0
62	65667.5
63	60534.0
64	53561.5
65	46589.0
66	40182.5
67	33776.0
68	33776.0
69	28807.0
70	23838.0
71	20576.5
72	17315.0
73	13905.5
74	10496.0
75	10496.0
76	8292.0
77	6088.0
78	4970.5
79	3853.0
80	2960.5
81	2068.0
82	2068.0
83	1571.5
84	1075.0
85	879.0
86	683.0
87	591.0
88	499.0
89	499.0
90	361.5
91	224.0
92	124.5
93	25.0
94	15.0
95	5.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1758004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.24066114586824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.52609709926872	45.534659547907914
2	8.920124491935672	9.498266509082146
3	2.2581450744935347	3.60675410187965
4	0.9405813226245847	2.0030868591195237
5	0.4955338525320832	1.3191274964483644
6	0.3251855393543381	1.0387855866180429
7	0.21761935848290923	0.8110338966638859
8	0.16816448152856964	0.7162550542266556
9	0.11988064097959139	0.574427212590952
>10	0.7867048929089073	8.233644165985893
>50	0.10610379631607668	4.009815852611245
>100	0.11911870624660727	13.471360925271627
>500	0.01191168275295455	4.215679121105484
>1k	0.00450712320382064	3.8287686095162075
>5k	3.219373717014743E-4	1.1383350609723477
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7720	0.4391343819467987	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6822	0.38805372456490433	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5382	0.3061426481395947	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4304	0.24482310620453648	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3152	0.17929424506428882	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3076	0.17497116047517525	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2676	0.15221808369036702	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2240	0.12741722999492605	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2119	0.12053442426752158	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1990	0.11319655700442093	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1866	0.10614310320113038	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1822	0.10364026475480147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.844134598101028E-4	0.0
3	0.0	0.0	0.0	2.844134598101028E-4	0.0
4	0.0	0.0	0.0	9.670057633543496E-4	0.0
5	0.0	5.688269196202056E-5	0.0	0.0010807711472783908	0.0
6	0.0	5.688269196202056E-5	0.0	0.0011945365312024318	0.0
7	0.0	5.688269196202056E-5	0.0	0.0014220672990505141	0.0
8	0.0	5.688269196202056E-5	0.0	0.001592715374936576	0.0
9	0.0	5.688269196202056E-5	0.0	0.002616603830252946	0.0
10	0.0	5.688269196202056E-5	0.0	0.004379967281075583	0.0
11	0.0	5.688269196202056E-5	0.0	0.005460738428353974	0.0
12	0.0	5.688269196202056E-5	0.0	0.00699657111132853	0.0
13	0.0	5.688269196202056E-5	0.0	0.007508515338986715	0.0
14	0.0	5.688269196202056E-5	0.0	0.008816817254113188	0.0
15	0.0	5.688269196202056E-5	0.0	0.010580180704935825	0.0
16	0.0	5.688269196202056E-5	0.0	0.0160409191332898	0.0
17	0.0	5.688269196202056E-5	0.0	0.021501657561643774	0.0
18	0.0	5.688269196202056E-5	0.0	0.023663199856200554	0.0
19	0.0	5.688269196202056E-5	0.0	0.027644988293541995	0.0
20	0.0	5.688269196202056E-5	0.0	0.03037535750771898	0.0
21	0.0	5.688269196202056E-5	0.0	0.037599459386895594	0.0
22	0.0	5.688269196202056E-5	0.0	0.055688155430818134	0.0
23	0.0	1.1376538392404112E-4	0.0	0.0865754571661953	0.0
24	0.0	1.1376538392404112E-4	0.0	0.1353239241776469	0.0
25	0.0	1.1376538392404112E-4	0.0	0.14311685297644375	0.0
26	0.0	1.1376538392404112E-4	0.0	0.15927153749365758	0.0
27	0.0	1.1376538392404112E-4	0.0	0.17195637780118816	0.0
28	0.0	1.1376538392404112E-4	0.0	0.20699611604979284	0.0
29	0.0	1.1376538392404112E-4	0.0	0.2613759695654845	0.0
30	0.0	1.1376538392404112E-4	0.0	0.3449935267496547	0.0
31	0.0	1.706480758860617E-4	0.0	0.6593272825317804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	230	0.0	20.913042	11
TACACCG	55	5.144585E-4	20.181818	5
CGGTGAT	280	0.0	18.5	14
CAGTCGG	250	0.0	18.5	10
TGTACCG	150	1.2732926E-11	18.5	5
GCGTATA	70	1.21966375E-4	18.5	9
CCGCTTA	95	3.6085858E-6	17.526316	25
GCAGTCG	275	0.0	17.490908	9
AACCACG	75	2.0680515E-4	17.266666	7
CGGGTAA	190	1.8189894E-12	16.552631	24
GCGGGTA	190	1.8189894E-12	16.552631	23
GTATTAG	340	0.0	16.32353	1
AGCGTAT	80	3.3838407E-4	16.1875	8
GTCGGTG	300	0.0	16.033333	12
ACGAACG	105	9.348654E-6	15.857142	15
GGTATCA	3600	0.0	15.262499	1
CTTATAC	1410	0.0	15.219858	37
TCGGTGA	305	0.0	15.163934	13
CGAATTA	135	3.9753286E-7	15.074073	15
CGCGGGT	210	9.094947E-12	14.97619	22
>>END_MODULE