FastQCFastQC Report
Fri 10 Feb 2017
ERR1630735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038445
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.10698688905045525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10750.10352016717303275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT603.7258396E-521.5833341
TAGACCC500.00703443618.55
TTATACC852.7227055E-517.4117644
TAGGACA1705.456968E-1217.4117644
GCGTATA650.001579795617.0769219
GCCGTCT1901.8189894E-1216.55263136
TTAGGAC2800.016.5178573
TTTCGAC904.4452903E-516.44444531
TGTTATA904.4452903E-516.4444452
GGTATCA8250.016.3696981
CTATACT1251.6571357E-716.284
ACTGTCC2400.016.18758
GCTTAGG1951.8189894E-1216.1282061
TATACTC1059.341278E-615.8571445
GTTAATC855.3635315E-415.23529435
TACACTA855.3635315E-415.2352945
GTATAGT1101.4515208E-515.1363641
CTAGGAT1101.4515208E-515.1363644
TAGCGTA750.004104050314.87
TCACGTA1001.09320215E-414.825
Produced by FastQC (version 0.11.5)