##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630735.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038445
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13209077033449	33.0	31.0	34.0	30.0	34.0
2	32.31629888920453	34.0	31.0	34.0	30.0	34.0
3	32.42395312221639	34.0	31.0	34.0	30.0	34.0
4	35.986517340831725	37.0	35.0	37.0	35.0	37.0
5	35.9347639980933	37.0	35.0	37.0	35.0	37.0
6	36.00480526171343	37.0	35.0	37.0	35.0	37.0
7	35.957022278502954	37.0	35.0	37.0	35.0	37.0
8	35.951255964446844	37.0	35.0	37.0	35.0	37.0
9	37.69157827328361	39.0	37.0	39.0	35.0	39.0
10	37.59346715521766	39.0	37.0	39.0	35.0	39.0
11	37.68706864590806	39.0	37.0	39.0	35.0	39.0
12	37.6336243132761	39.0	37.0	39.0	35.0	39.0
13	37.6659004569332	39.0	37.0	39.0	35.0	39.0
14	38.99358078665697	40.0	38.0	41.0	36.0	41.0
15	39.01254279234817	40.0	38.0	41.0	36.0	41.0
16	38.98558036294652	40.0	38.0	41.0	36.0	41.0
17	38.95121744531487	40.0	38.0	41.0	36.0	41.0
18	38.92914790865188	40.0	38.0	41.0	35.0	41.0
19	38.975828281709674	40.0	38.0	41.0	35.0	41.0
20	38.93968481720265	40.0	38.0	41.0	35.0	41.0
21	38.895330999715924	40.0	38.0	41.0	35.0	41.0
22	38.834516994159536	40.0	38.0	41.0	35.0	41.0
23	38.790219029414175	40.0	38.0	41.0	35.0	41.0
24	38.7499684624607	40.0	38.0	41.0	35.0	41.0
25	38.69588567521631	40.0	38.0	41.0	34.0	41.0
26	38.58326825204994	40.0	38.0	41.0	34.0	41.0
27	38.473299982184905	40.0	38.0	41.0	34.0	41.0
28	38.38512294825436	40.0	38.0	41.0	34.0	41.0
29	38.32344515116352	40.0	38.0	41.0	34.0	41.0
30	38.24795343036945	40.0	38.0	41.0	34.0	41.0
31	38.22924853988415	40.0	38.0	41.0	34.0	41.0
32	38.15011676111879	40.0	38.0	41.0	33.0	41.0
33	38.09110352498207	40.0	38.0	41.0	33.0	41.0
34	38.04811039583223	40.0	38.0	41.0	33.0	41.0
35	38.00451733120194	40.0	37.0	41.0	33.0	41.0
36	37.98979146704929	40.0	37.0	41.0	33.0	41.0
37	37.87355131952101	40.0	37.0	41.0	33.0	41.0
38	37.75514735975425	40.0	37.0	41.0	33.0	41.0
39	37.71591080894992	40.0	37.0	41.0	33.0	41.0
40	37.5741825517962	40.0	37.0	41.0	32.0	41.0
41	37.53640780205018	40.0	37.0	41.0	32.0	41.0
42	37.42700672640342	40.0	36.0	41.0	32.0	41.0
43	36.608496357534584	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	9.0
17	4.0
18	27.0
19	47.0
20	90.0
21	209.0
22	399.0
23	659.0
24	1111.0
25	1824.0
26	2773.0
27	4232.0
28	6228.0
29	8832.0
30	12297.0
31	16480.0
32	22188.0
33	29171.0
34	39334.0
35	54096.0
36	77396.0
37	119774.0
38	232037.0
39	409225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73654839688187	17.99739032880894	11.6347038119496	26.631357462359585
2	19.412101748287103	20.329916365334707	33.62171323469226	26.636268651685935
3	20.60532815892994	22.053743818883042	28.562899335063484	28.778028687123538
4	15.059536133353236	14.893037185407026	33.08504542850127	36.96238125273847
5	15.38107458748417	35.7073316352816	33.775115677768206	15.136478099466027
6	36.77710422795622	33.40870243489063	14.947349161486645	14.866844175666502
7	30.67432555407364	29.19499829071352	20.434977297786595	19.695698857426247
8	27.89950358468672	32.64265319781019	19.51475523499078	19.943087982512314
9	27.209337037589858	13.41419141119655	18.255564810846987	41.12090674036661
10	17.26976392586993	25.94706508288836	32.23993567304961	24.543235318192107
11	37.2794900066927	20.486111445478574	20.70884832610297	21.52555022172575
12	22.901164722252982	24.06829442098522	27.835754421274117	25.194786435487675
13	31.147051601192167	18.756506122134535	24.01725657112317	26.079185705550124
14	23.888410074678966	19.716884380010498	23.691384714645455	32.703320830665085
15	26.657261578610324	26.58186037777639	20.95190404884226	25.80897399477103
16	26.82096788948861	25.243898328751165	22.3129775770503	25.622156204709928
17	25.081636485321802	25.428790162213694	23.957840810057345	25.53173254240716
18	25.148370881462185	24.174125736076537	25.021835532936265	25.65566784952501
19	25.94128721309265	24.374521520157543	24.612569755740555	25.07162151100925
20	26.16200183928855	23.78219356826794	24.606406694625136	25.449397897818372
21	26.369716258444114	24.174992416545894	23.810697725926747	25.644593599083244
22	26.435872867604925	24.13291026486718	23.827549846164217	25.603667021363673
23	25.528843607509305	24.346113660328665	24.234889666761358	25.89015306540067
24	25.989339830226925	24.422863030781603	24.05356085300618	25.534236285985294
25	26.156705458642488	23.952544429411283	24.109220998704796	25.78152911324143
26	25.954961504942485	24.49113819220084	24.199644661007564	25.35425564184911
27	26.240677166340053	23.939833115860733	23.80434206915147	26.015147648647734
28	25.46933154861355	24.13117690392847	24.538035235375972	25.86145631208201
29	25.181208441467774	24.496530870676832	24.652437057330914	25.669823630524487
30	25.0250133613239	25.12959280462615	24.570295008401985	25.275098825647962
31	25.76795111922153	24.717245496872728	23.880032163475196	25.634771220430547
32	25.063628791125193	24.430663155005803	24.381936453062032	26.12377160080698
33	25.01634655663035	24.32146141586699	24.62296992137282	26.039222106129838
34	25.870508308095275	23.908921512453716	24.464752586800458	25.755817592650548
35	25.509006254544055	24.131369499588327	25.108503579871826	25.251120665995792
36	25.048028542676793	24.505197675370386	24.58637674600003	25.860397035952793
37	25.60183736259503	23.624650318505072	24.993138779617603	25.78037353928229
38	24.927174766116643	23.618679853049514	25.65807529527322	25.796070085560626
39	25.080192017872875	23.511018879189557	25.462012913538995	25.946776189398573
40	24.496241977187044	23.44958086369524	26.3856054003823	25.668571758735414
41	23.969878038798395	23.542026780426504	26.580608505987318	25.907486674787783
42	24.49999759255425	23.233199639846116	26.60140883725185	25.66539393034778
43	23.8461353273404	22.938528280265206	26.826745759284314	26.38859063311008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	16.0
2	20.0
3	80.5
4	141.0
5	141.0
6	215.5
7	290.0
8	300.5
9	311.0
10	434.5
11	558.0
12	558.0
13	934.5
14	1311.0
15	2082.5
16	2854.0
17	2937.0
18	3020.0
19	3020.0
20	3652.0
21	4284.0
22	4700.0
23	5116.0
24	6288.0
25	7460.0
26	7460.0
27	9060.5
28	10661.0
29	13540.0
30	16419.0
31	19495.0
32	22571.0
33	22571.0
34	26141.0
35	29711.0
36	33996.0
37	38281.0
38	43886.0
39	49491.0
40	49491.0
41	52876.5
42	56262.0
43	57764.5
44	59267.0
45	63019.0
46	66771.0
47	66771.0
48	69376.0
49	71981.0
50	75837.0
51	79693.0
52	82882.0
53	86071.0
54	86071.0
55	83010.0
56	79949.0
57	77050.5
58	74152.0
59	69504.5
60	64857.0
61	64857.0
62	60897.5
63	56938.0
64	51034.5
65	45131.0
66	39633.0
67	34135.0
68	34135.0
69	29538.0
70	24941.0
71	21622.5
72	18304.0
73	14712.5
74	11121.0
75	11121.0
76	8893.5
77	6666.0
78	5519.5
79	4373.0
80	3450.0
81	2527.0
82	2527.0
83	1935.0
84	1343.0
85	1048.5
86	754.0
87	602.5
88	451.0
89	451.0
90	324.5
91	198.0
92	114.5
93	31.0
94	23.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1038445.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.89582296645433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.39570770210221	56.067205520971605
2	8.374618722849595	10.869555480992025
3	2.146934831113433	4.179813081613556
4	0.8993342849728907	2.3345215418105405
5	0.5177403334805495	1.6799592512073382
6	0.3073780600814886	1.196853130249227
7	0.2146194773988991	0.9749535327302333
8	0.160989795656933	0.8358052222686386
9	0.1122772307441007	0.6557690960579613
>10	0.7066325364184635	8.836191788013025
>50	0.10151278239773392	4.673433865929457
>100	0.06091703981633113	7.010385280470555
>500	0.0010400467524969508	0.47477693314278097
>1k	2.971562149991288E-4	0.21077627454307862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1111	0.10698688905045525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1075	0.10352016717303275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.629782992840256E-5	0.0
2	0.0	0.0	0.0	0.0023111479182816616	0.0
3	0.0	0.0	0.0	0.004429700176706518	0.0
4	0.0	0.0	0.0	0.007800124224200608	0.0
5	0.0	0.0	0.0	0.008955698183341439	0.0
6	0.0	0.0	0.0	0.009244591673126646	9.629782992840256E-5
7	0.0	0.0	0.0	0.010400165632267477	9.629782992840256E-5
8	0.0	0.0	0.0	0.0112668461016231	9.629782992840256E-5
9	0.0	0.0	0.0	0.020222544284964537	9.629782992840256E-5
10	0.0	0.0	0.0	0.032259773026014855	9.629782992840256E-5
11	0.0	0.0	0.0	0.036593175372792976	9.629782992840256E-5
12	0.0	0.0	0.0	0.03900062112100304	9.629782992840256E-5
13	0.0	0.0	0.0	0.040637684229785885	9.629782992840256E-5
14	0.0	9.629782992840256E-5	0.0	0.04275623648821074	9.629782992840256E-5
15	0.0	9.629782992840256E-5	0.0	0.04554887355613441	9.629782992840256E-5
16	0.0	9.629782992840256E-5	0.0	0.04766742581455927	9.629782992840256E-5
17	0.0	9.629782992840256E-5	0.0	0.050652658542339746	9.629782992840256E-5
18	0.0	1.9259565985680512E-4	0.0	0.05334899778033502	9.629782992840256E-5
19	0.0	1.9259565985680512E-4	0.0	0.055082358719046265	9.629782992840256E-5
20	0.0	1.9259565985680512E-4	0.0	0.06201580247389125	9.629782992840256E-5
21	0.0	1.9259565985680512E-4	0.0	0.06692699180023978	9.629782992840256E-5
22	0.0	1.9259565985680512E-4	0.0	0.07347524423537116	1.9259565985680512E-4
23	0.0	1.9259565985680512E-4	0.0	0.08069758148000135	1.9259565985680512E-4
24	0.0	1.9259565985680512E-4	0.0	0.08955698183341439	1.9259565985680512E-4
25	0.0	1.9259565985680512E-4	0.0	0.09456446898969131	1.9259565985680512E-4
26	0.0	1.9259565985680512E-4	0.0	0.10014974312553866	1.9259565985680512E-4
27	0.0	1.9259565985680512E-4	0.0	0.10997212177823573	1.9259565985680512E-4
28	0.0	1.9259565985680512E-4	0.0	0.13327619662090914	1.9259565985680512E-4
29	0.0	1.9259565985680512E-4	0.0	0.16736562841556366	1.9259565985680512E-4
30	0.0	1.9259565985680512E-4	0.0	0.21734420214840458	1.9259565985680512E-4
31	0.0	1.9259565985680512E-4	0.0	0.40685833144750083	1.9259565985680512E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAT	60	3.7258396E-5	21.583334	1
TAGACCC	50	0.007034436	18.5	5
TTATACC	85	2.7227055E-5	17.411764	4
TAGGACA	170	5.456968E-12	17.411764	4
GCGTATA	65	0.0015797956	17.076921	9
GCCGTCT	190	1.8189894E-12	16.552631	36
TTAGGAC	280	0.0	16.517857	3
TTTCGAC	90	4.4452903E-5	16.444445	31
TGTTATA	90	4.4452903E-5	16.444445	2
GGTATCA	825	0.0	16.369698	1
CTATACT	125	1.6571357E-7	16.28	4
ACTGTCC	240	0.0	16.1875	8
GCTTAGG	195	1.8189894E-12	16.128206	1
TATACTC	105	9.341278E-6	15.857144	5
GTTAATC	85	5.3635315E-4	15.235294	35
TACACTA	85	5.3635315E-4	15.235294	5
GTATAGT	110	1.4515208E-5	15.136364	1
CTAGGAT	110	1.4515208E-5	15.136364	4
TAGCGTA	75	0.0041040503	14.8	7
TCACGTA	100	1.09320215E-4	14.8	25
>>END_MODULE