FastQCFastQC Report
Fri 10 Feb 2017
ERR1630734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766691
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG46130.2611096111317712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39840.22550632793170963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38970.2205818674572973No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG35880.2030915423240397No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT35650.20178967346298815No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA29150.16499772738979257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28880.16346944655290596No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC28720.16256379864956577No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT23470.13284722682121547No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC22530.1275265453890918No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC21420.12124361305966917No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC20410.11552671066983418No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC20070.11360220887523624No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC19680.11139469211084452No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC19610.11099847115313317No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA19330.10941358732228783No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC18920.10709286456997856No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG18760.10618721666663836No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT18260.10335706696870024No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA18160.10279103702911263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.10182878613181365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCG358.8712876E-426.4285725
ATGCGTT400.001931595523.12536
CAAGACG3300.017.9393944
TAGTACC852.7244567E-517.4117644
GGTATCA21450.016.9044281
CGGCCTT7800.016.83974524
ATCTCGC8000.016.65000211
TCTCGCC7950.016.52201312
AAGACGG3150.015.8571425
TTAACGG1059.3487E-615.85714235
TCGCCAT6800.015.77941213
TCCTCGG8800.015.76704520
TAACGCC2003.6379788E-1215.7254
TTCGGAA2850.015.57894731
GGCTTAC1201.9368345E-615.4166661
AACGCCG2055.456968E-1215.3414635
TCTTATA10100.015.202970537
CGACGGT2109.094947E-1214.976197
TTCCTCG8850.014.84180719
GCTTAGG10850.014.8341021
Produced by FastQC (version 0.11.5)