##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630734.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1766691
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10623589524144	33.0	31.0	34.0	30.0	34.0
2	32.29815570464784	34.0	31.0	34.0	30.0	34.0
3	32.39226893667313	34.0	31.0	34.0	30.0	34.0
4	35.959457539547095	37.0	35.0	37.0	35.0	37.0
5	35.91557040818117	37.0	35.0	37.0	35.0	37.0
6	35.986131134420226	37.0	35.0	37.0	35.0	37.0
7	35.942994558754194	37.0	35.0	37.0	35.0	37.0
8	35.93377449706825	37.0	35.0	37.0	35.0	37.0
9	37.662300311712684	39.0	37.0	39.0	35.0	39.0
10	37.579671827161626	39.0	37.0	39.0	35.0	39.0
11	37.66368142476528	39.0	37.0	39.0	35.0	39.0
12	37.61505435868525	39.0	37.0	39.0	35.0	39.0
13	37.63639764961728	39.0	37.0	39.0	35.0	39.0
14	38.97467016020345	40.0	38.0	41.0	36.0	41.0
15	39.00432503476839	40.0	38.0	41.0	36.0	41.0
16	38.95933584311008	40.0	38.0	41.0	36.0	41.0
17	38.9405068571697	40.0	38.0	41.0	36.0	41.0
18	38.91083783185628	40.0	38.0	41.0	35.0	41.0
19	38.986637731216156	40.0	38.0	41.0	35.0	41.0
20	38.94793656615673	40.0	38.0	41.0	35.0	41.0
21	38.90118192711685	40.0	38.0	41.0	35.0	41.0
22	38.85285146072516	40.0	38.0	41.0	35.0	41.0
23	38.80655870211599	40.0	38.0	41.0	35.0	41.0
24	38.764643619059584	40.0	38.0	41.0	35.0	41.0
25	38.71454996940608	40.0	38.0	41.0	34.0	41.0
26	38.59716894465416	40.0	38.0	41.0	34.0	41.0
27	38.48439030934102	40.0	38.0	41.0	34.0	41.0
28	38.39781376596134	40.0	38.0	41.0	34.0	41.0
29	38.346598810997506	40.0	38.0	41.0	34.0	41.0
30	38.2786723880973	40.0	38.0	41.0	34.0	41.0
31	38.24842657827543	40.0	38.0	41.0	34.0	41.0
32	38.161387588435105	40.0	38.0	41.0	33.0	41.0
33	38.10176595680852	40.0	38.0	41.0	33.0	41.0
34	38.05728336194615	40.0	38.0	41.0	33.0	41.0
35	38.00944194542226	40.0	38.0	41.0	33.0	41.0
36	37.989434485147655	40.0	38.0	41.0	33.0	41.0
37	37.88962416178041	40.0	37.0	41.0	33.0	41.0
38	37.746676130687256	40.0	37.0	41.0	33.0	41.0
39	37.71258641154565	40.0	37.0	41.0	33.0	41.0
40	37.57146382700767	40.0	37.0	41.0	32.0	41.0
41	37.526734443091634	40.0	37.0	41.0	32.0	41.0
42	37.42784335234628	40.0	37.0	41.0	32.0	41.0
43	36.63426598086479	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	4.0
11	3.0
12	2.0
13	5.0
14	0.0
15	2.0
16	8.0
17	17.0
18	39.0
19	105.0
20	167.0
21	343.0
22	684.0
23	1184.0
24	1987.0
25	3266.0
26	4972.0
27	7559.0
28	11001.0
29	15598.0
30	21748.0
31	28835.0
32	37662.0
33	49602.0
34	65902.0
35	89812.0
36	127979.0
37	201421.0
38	391393.0
39	705385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.436571534014725	18.795646776940618	12.251095409440586	25.516686279604073
2	18.249201473262726	21.194481660912974	35.57424586416074	24.98207100166356
3	19.916612469299952	23.31698072837865	30.28079047213123	26.48561633019017
4	14.601704542560073	15.506843018954644	34.221434308546314	35.67001812993896
5	14.01654279101439	37.562992056901855	34.52907158071219	13.891393571371562
6	34.916802089329714	34.559863609425754	15.897064059306354	14.626270241938178
7	28.7738489639671	30.88825380329667	21.40045995592891	18.93743727680732
8	26.258015691481983	34.77110598287986	20.283626282128566	18.687252043509588
9	26.308165944129446	14.344896758969169	19.38007268956484	39.96686460733654
10	16.402358986376225	27.5122814346142	33.28952261600925	22.795836963000323
11	35.12074267656313	22.0108666427802	21.75751164182078	21.11087903883588
12	21.131425925642912	25.98139686000551	28.930978875196622	23.956198339154952
13	29.33506764906823	20.037799479365663	26.087867091641947	24.539265779924165
14	22.83653451565667	21.081275672995446	24.78599823059041	31.296191580757473
15	25.468064307793497	28.04559484369366	22.802629322275372	23.68371152623747
16	25.438064720995353	26.791442306549364	23.598014593383905	24.172478379071382
17	24.036970811534104	26.94296852137697	24.92863777536649	24.091422891722438
18	22.6888007014243	25.631533754346403	27.111928458343876	24.567737085885422
19	24.530152697896803	25.92490707203467	26.5636718588593	22.981268371209225
20	24.6409247570741	24.868525395782285	27.083060931424907	23.40748891571871
21	24.235986938293113	26.156073699362253	25.475762314971888	24.132177047372743
22	24.429399368650206	25.352820612093456	25.633628065122878	24.584151954133464
23	23.435847015692048	26.2426196771252	26.104281959889985	24.217251347292766
24	24.849676598794016	26.1676773131238	25.756796179977144	23.22584990810504
25	24.64194361096536	25.4174046282004	26.035962146181763	23.904689614652476
26	24.569604984686062	25.95745379356096	25.68417453872805	23.78876668302493
27	24.791375515016494	25.103088202747397	25.56078001189795	24.544756270338162
28	23.486676504267017	25.56819500410655	26.587048895364273	24.358079596262165
29	23.596995739492645	26.224676528040273	26.10965924431607	24.068668488151012
30	23.109870373483535	27.14973925830833	26.159922702951448	23.580467665256684
31	24.2740241502334	26.062565553342377	25.94743506363025	23.715975232793962
32	23.204001152436955	26.05464113418815	26.078244582668958	24.663113130705934
33	23.34981046487473	25.636288405838936	26.440050920053366	24.573850209232965
34	23.971650956505695	25.579289190922466	26.324694018365406	24.124365834206436
35	24.261911109526228	25.63017528249139	26.299222671083967	23.808690936898415
36	23.43663945760747	26.39516474584407	26.25592138070551	23.91227441584295
37	24.93826028433948	24.868412189794366	25.951850097159042	24.241477428707114
38	23.79606846924561	25.37970703422387	26.998835676414267	23.82538882011625
39	23.94436831341757	25.246180571475147	26.776385910156332	24.03306520495095
40	23.42537546180968	24.707376671981688	27.601487753093213	24.265760113115423
41	22.645272999070013	25.52121451912077	27.910087276156386	23.92342520565283
42	23.883406888923982	24.535869600286638	27.42143362931039	24.15928988147899
43	22.856232357554322	24.6619244678328	27.568431604621296	24.913411569991585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	64.0
2	84.0
3	305.0
4	526.0
5	526.0
6	819.5
7	1113.0
8	1148.5
9	1184.0
10	1699.5
11	2215.0
12	2215.0
13	3678.5
14	5142.0
15	8012.0
16	10882.0
17	10716.5
18	10551.0
19	10551.0
20	12378.0
21	14205.0
22	14951.5
23	15698.0
24	18003.0
25	20308.0
26	20308.0
27	23523.0
28	26738.0
29	36817.5
30	46897.0
31	49898.0
32	52899.0
33	52899.0
34	58579.5
35	64260.0
36	68617.0
37	72974.0
38	87593.0
39	102212.0
40	102212.0
41	109688.5
42	117165.0
43	110249.0
44	103333.0
45	111085.5
46	118838.0
47	118838.0
48	125471.0
49	132104.0
50	137290.0
51	142476.0
52	151348.0
53	160220.0
54	160220.0
55	138703.5
56	117187.0
57	109185.5
58	101184.0
59	90625.5
60	80067.0
61	80067.0
62	75300.0
63	70533.0
64	62523.5
65	54514.0
66	47551.0
67	40588.0
68	40588.0
69	34443.0
70	28298.0
71	25040.0
72	21782.0
73	17271.0
74	12760.0
75	12760.0
76	10024.5
77	7289.0
78	6019.0
79	4749.0
80	3627.5
81	2506.0
82	2506.0
83	1932.5
84	1359.0
85	1120.0
86	881.0
87	737.5
88	594.0
89	594.0
90	445.0
91	296.0
92	159.0
93	22.0
94	13.5
95	5.0
96	5.0
97	5.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1766691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.08820586877844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.57193977943564	42.005729964836476
2	8.864290133285614	8.702641978866115
3	2.196079772133058	3.234048478761828
4	0.9218311773962415	1.8100415444914049
5	0.509726738328818	1.2510785533952986
6	0.3160952129783487	0.9309928133289926
7	0.2267860557587189	0.7792764415276582
8	0.1513190045282965	0.5942382756914908
9	0.11804451338456678	0.5215134037231247
>10	0.8321693989665695	8.073133255684315
>50	0.12061566214284086	4.171957658186147
>100	0.14426307788393522	15.415158704951622
>500	0.01966685578518395	6.424580823948811
>1k	0.007172617992243557	6.085608102606735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4613	0.2611096111317712	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3984	0.22550632793170963	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3897	0.2205818674572973	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3588	0.2030915423240397	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3565	0.20178967346298815	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2915	0.16499772738979257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2888	0.16346944655290596	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2872	0.16256379864956577	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2347	0.13284722682121547	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2253	0.1275265453890918	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2142	0.12124361305966917	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2041	0.11552671066983418	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2007	0.11360220887523624	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1968	0.11139469211084452	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1961	0.11099847115313317	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1933	0.10941358732228783	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1892	0.10709286456997856	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1876	0.10618721666663836	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1826	0.10335706696870024	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1816	0.10279103702911263	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1799	0.10182878613181365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.6602993958762455E-5	0.0	0.0	5.094269456288621E-4	0.0
3	5.6602993958762455E-5	0.0	0.0	8.490449093814368E-4	0.0
4	5.6602993958762455E-5	0.0	0.0	0.0016980898187628735	0.0
5	5.6602993958762455E-5	0.0	0.0	0.001754692812721636	0.0
6	5.6602993958762455E-5	0.0	0.0	0.001754692812721636	0.0
7	5.6602993958762455E-5	0.0	0.0	0.0022075167643917358	0.0
8	5.6602993958762455E-5	0.0	0.0	0.0022075167643917358	0.0
9	5.6602993958762455E-5	0.0	0.0	0.0027735467039793605	0.0
10	5.6602993958762455E-5	0.0	0.0	0.0037924005952370845	0.0
11	5.6602993958762455E-5	0.0	0.0	0.004528239516700996	0.0
12	5.6602993958762455E-5	0.0	0.0	0.005207475444206145	0.0
13	5.6602993958762455E-5	0.0	0.0	0.005716902389835008	0.0
14	5.6602993958762455E-5	0.0	0.0	0.006056520353587583	0.0
15	1.1320598791752491E-4	0.0	0.0	0.0066791532871339696	0.0
16	1.1320598791752491E-4	0.0	0.0	0.00826403711797932	0.0
17	1.6980898187628736E-4	0.0	0.0	0.00967911196694838	5.6602993958762455E-5
18	1.6980898187628736E-4	0.0	0.0	0.010301744900494767	5.6602993958762455E-5
19	1.6980898187628736E-4	0.0	0.0	0.011377201785711253	5.6602993958762455E-5
20	1.6980898187628736E-4	0.0	0.0	0.012905482622597839	5.6602993958762455E-5
21	1.6980898187628736E-4	0.0	0.0	0.015622426332618438	5.6602993958762455E-5
22	1.6980898187628736E-4	0.0	0.0	0.019980856867443147	5.6602993958762455E-5
23	1.6980898187628736E-4	5.6602993958762455E-5	0.0	0.027112834106247215	5.6602993958762455E-5
24	1.6980898187628736E-4	5.6602993958762455E-5	0.0	0.03690515206111312	5.6602993958762455E-5
25	1.6980898187628736E-4	5.6602993958762455E-5	0.0	0.038942859843628566	5.6602993958762455E-5
26	2.2641197583504982E-4	5.6602993958762455E-5	0.0	0.0438673203180409	5.6602993958762455E-5
27	2.2641197583504982E-4	5.6602993958762455E-5	0.0	0.05071628258705116	5.6602993958762455E-5
28	2.2641197583504982E-4	5.6602993958762455E-5	0.0	0.06933866759948401	5.6602993958762455E-5
29	2.2641197583504982E-4	5.6602993958762455E-5	0.0	0.09797978254261781	5.6602993958762455E-5
30	2.2641197583504982E-4	5.6602993958762455E-5	0.0	0.14026221902981337	5.6602993958762455E-5
31	2.2641197583504982E-4	5.6602993958762455E-5	0.0	0.30220338474583275	5.6602993958762455E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGCG	35	8.8712876E-4	26.428572	5
ATGCGTT	40	0.0019315955	23.125	36
CAAGACG	330	0.0	17.939394	4
TAGTACC	85	2.7244567E-5	17.411764	4
GGTATCA	2145	0.0	16.904428	1
CGGCCTT	780	0.0	16.839745	24
ATCTCGC	800	0.0	16.650002	11
TCTCGCC	795	0.0	16.522013	12
AAGACGG	315	0.0	15.857142	5
TTAACGG	105	9.3487E-6	15.857142	35
TCGCCAT	680	0.0	15.779412	13
TCCTCGG	880	0.0	15.767045	20
TAACGCC	200	3.6379788E-12	15.725	4
TTCGGAA	285	0.0	15.578947	31
GGCTTAC	120	1.9368345E-6	15.416666	1
AACGCCG	205	5.456968E-12	15.341463	5
TCTTATA	1010	0.0	15.2029705	37
CGACGGT	210	9.094947E-12	14.97619	7
TTCCTCG	885	0.0	14.841807	19
GCTTAGG	1085	0.0	14.834102	1
>>END_MODULE