FastQCFastQC Report
Fri 10 Feb 2017
ERR1630733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081788
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95070.8788228377463977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85750.7926691736273651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65860.6088069011673267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47760.441491308833154No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15990.14781084648748186No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC13890.12839854019456678No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.1235916833982259No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.12072605723117653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.12063361767740075No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT12910.11933946392453974No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11790.10898623390165171No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11660.10778451970256649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA250.005496203429.59999829
TTAACGG405.939811E-527.75000235
GCAGCGT659.390715E-825.6153851
GGACCGT400.001931113923.1250026
TCTAGCG1500.022.228
GCCGTCT1500.022.236
TAACGGC502.7019606E-422.19999936
CTAGCGG1550.021.48387129
GACCGTG555.1428244E-420.1818187
CAGCGTC851.2451692E-619.5882362
AGCGTCA902.1519772E-618.53
TTATGCG609.236337E-418.54
CGGTCCA2150.018.06976710
GCGGCGC2100.016.73809632
CGCAATA2050.016.24390436
GCGTTAT1606.2937033E-1016.18751
ATGCCGT2200.015.97727334
TGCCGTC2200.015.97727335
CCGGTCC2550.015.9607869
ACCGTGA700.002592362215.8571428
Produced by FastQC (version 0.11.5)