##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1081788
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094774576904165	33.0	31.0	34.0	30.0	34.0
2	32.248662399656865	33.0	31.0	34.0	30.0	34.0
3	32.33313643708379	34.0	31.0	34.0	30.0	34.0
4	35.90499247542032	37.0	35.0	37.0	35.0	37.0
5	35.87360277614468	37.0	35.0	37.0	35.0	37.0
6	35.95877750538923	37.0	35.0	37.0	35.0	37.0
7	35.9083850070439	37.0	35.0	37.0	35.0	37.0
8	35.9242790639201	37.0	35.0	37.0	35.0	37.0
9	37.62152011299811	39.0	37.0	39.0	35.0	39.0
10	37.54498478444945	39.0	37.0	39.0	35.0	39.0
11	37.646686781513566	39.0	37.0	39.0	35.0	39.0
12	37.604440981042494	39.0	37.0	39.0	35.0	39.0
13	37.64339593339915	39.0	37.0	39.0	35.0	39.0
14	38.91896009199584	40.0	38.0	41.0	36.0	41.0
15	38.92044929320717	40.0	38.0	41.0	36.0	41.0
16	38.866145677341585	40.0	38.0	41.0	35.0	41.0
17	38.887688715349036	40.0	38.0	41.0	35.0	41.0
18	38.88995440881208	40.0	38.0	41.0	35.0	41.0
19	38.96044511493934	40.0	38.0	41.0	35.0	41.0
20	38.94397331085204	40.0	38.0	41.0	35.0	41.0
21	38.90800508047788	40.0	38.0	41.0	35.0	41.0
22	38.81439893953344	40.0	38.0	41.0	35.0	41.0
23	38.73124493893443	40.0	38.0	41.0	35.0	41.0
24	38.66301900187467	40.0	38.0	41.0	34.0	41.0
25	38.616608799506004	40.0	38.0	41.0	34.0	41.0
26	38.46509020251658	40.0	38.0	41.0	34.0	41.0
27	38.311060947246595	40.0	38.0	41.0	34.0	41.0
28	38.177260239529375	40.0	38.0	41.0	34.0	41.0
29	38.08236918878745	40.0	37.0	41.0	33.0	41.0
30	37.96096832281371	40.0	37.0	41.0	33.0	41.0
31	37.882249572004866	40.0	37.0	41.0	33.0	41.0
32	37.73163873143351	40.0	37.0	41.0	33.0	41.0
33	37.590773793016744	40.0	36.0	41.0	33.0	41.0
34	37.50164265087059	40.0	36.0	41.0	33.0	41.0
35	37.39746512255636	40.0	36.0	41.0	33.0	41.0
36	37.31231257880472	40.0	36.0	41.0	32.0	41.0
37	37.130537591468936	40.0	35.0	41.0	32.0	41.0
38	36.91076902313577	40.0	35.0	41.0	31.0	41.0
39	36.78526199218331	39.0	35.0	41.0	31.0	41.0
40	36.53027302946603	39.0	35.0	41.0	30.0	41.0
41	36.39089082149183	39.0	35.0	41.0	30.0	41.0
42	36.15400706977707	39.0	35.0	41.0	29.0	41.0
43	35.37825341009514	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	9.0
12	5.0
13	3.0
14	9.0
15	3.0
16	6.0
17	15.0
18	37.0
19	79.0
20	147.0
21	318.0
22	622.0
23	1064.0
24	1772.0
25	2754.0
26	4263.0
27	6194.0
28	9075.0
29	12581.0
30	16390.0
31	21313.0
32	26972.0
33	34847.0
34	45711.0
35	60191.0
36	84831.0
37	136922.0
38	228730.0
39	386919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32772410121022	20.091644573613316	12.571409555291796	23.00922176988467
2	18.952419512880528	21.925368001863582	33.96127522213225	25.160937263123646
3	21.26276128039875	24.61831708245978	29.26811907693559	24.85080256020588
4	13.949868181196315	15.969487552089689	34.126742023390904	35.95390224332309
5	13.739290877695076	38.5773367794799	33.122478711170764	14.560893631654261
6	33.902113907715744	34.266233310038565	16.10870152007602	15.722951262169667
7	27.500952127403888	31.84450188022052	21.852710512595813	18.801835479779772
8	28.307025036328742	31.903848073744577	20.405014660913228	19.384112229013446
9	26.624717597163215	13.330708050006102	21.574097697515597	38.47047665531509
10	17.959433826221034	27.25432339793009	32.052953073984916	22.733289701863953
11	34.030420008356536	22.719885966566462	20.805925005638812	22.44376901943819
12	21.14360669558176	27.748782571076774	28.91481510240454	22.192795630936928
13	29.54830336443	20.251749880752975	26.67990401076736	23.520042744049665
14	22.49313174115446	22.51106501458696	26.777705058662143	28.21809818559644
15	25.817442974039274	27.69165492684334	24.364200749130145	22.126701349987243
16	24.103151449267322	26.508890836282156	26.14116629136208	23.246791423088442
17	21.706563578076295	27.311081283948425	27.11658846280417	23.865766675171106
18	21.683361250078573	24.772413818604015	29.86879129737065	23.675433633946763
19	21.871290862904747	26.53181584561855	29.52528591553983	22.071607375936875
20	23.06080304089156	25.02911845943937	29.72301412106623	22.18706437860283
21	24.76677500582369	25.669539687998018	28.155793926351556	21.407891379826733
22	23.791167955274044	25.604000044370984	28.705624392209934	21.899207608145034
23	22.743550492333064	25.8257625338791	28.7458355981024	22.68485137568544
24	22.14768512869435	26.718358865138086	28.77347502468136	22.360480981486205
25	22.379153771348914	26.58219540242635	28.635647650001665	22.40300317622307
26	22.369447618202457	27.089780992209196	28.630748353651548	21.910023035936803
27	22.73171822944976	26.176108442689326	28.894016202804984	22.198157125055925
28	21.593232685147182	26.344255991007483	29.91094373389241	22.15156758995293
29	21.139262036554296	26.91303656538989	29.940616830654438	22.007084567401375
30	21.15016990389984	27.978864620424705	29.563093693034126	21.307871782641328
31	21.99275643656613	27.88642506664892	28.16614715637445	21.954671340410506
32	21.162741683213344	27.27087007805596	28.642765495642397	22.923622743088295
33	20.85427089226355	27.21864173017264	29.29446434976169	22.632623027802122
34	21.91464501362559	26.725754029440147	29.130846339578547	22.228754617355712
35	21.57049255491834	26.731485281774248	29.672727003812206	22.025295159495208
36	20.80823599448321	28.097926765687912	28.513442559910075	22.5803946799188
37	21.81924739412898	27.076469696465484	28.861015282107026	22.24326762729851
38	21.056898394140074	26.579422215813082	29.324322325631268	23.039357064415576
39	21.47657396828214	26.44556974194574	29.374609442885298	22.70324684688682
40	20.747133449437413	26.53921100992061	30.165892023206027	22.547763517435946
41	20.260901396576777	26.940675991968853	30.225330656283855	22.57309195517051
42	20.774495557355046	26.820874330275434	30.338938867874298	22.065691244495227
43	20.026012490432507	26.01868388260916	30.02316535217621	23.93213827478212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	124.5
2	181.0
3	690.0
4	1199.0
5	1199.0
6	1813.0
7	2427.0
8	2631.0
9	2835.0
10	4125.5
11	5416.0
12	5416.0
13	9474.0
14	13532.0
15	18271.0
16	23010.0
17	21300.0
18	19590.0
19	19590.0
20	21624.5
21	23659.0
22	19782.5
23	15906.0
24	16880.5
25	17855.0
26	17855.0
27	20551.5
28	23248.0
29	25942.0
30	28636.0
31	31579.5
32	34523.0
33	34523.0
34	38011.0
35	41499.0
36	44924.0
37	48349.0
38	51611.5
39	54874.0
40	54874.0
41	57779.5
42	60685.0
43	61284.0
44	61883.0
45	64065.0
46	66247.0
47	66247.0
48	68779.0
49	71311.0
50	69447.5
51	67584.0
52	68314.0
53	69044.0
54	69044.0
55	68689.0
56	68334.0
57	65449.0
58	62564.0
59	58105.0
60	53646.0
61	53646.0
62	49222.5
63	44799.0
64	39381.5
65	33964.0
66	29233.0
67	24502.0
68	24502.0
69	20003.5
70	15505.0
71	13003.0
72	10501.0
73	7912.5
74	5324.0
75	5324.0
76	4466.0
77	3608.0
78	2843.0
79	2078.0
80	1632.0
81	1186.0
82	1186.0
83	926.0
84	666.0
85	647.0
86	628.0
87	617.0
88	606.0
89	606.0
90	441.0
91	276.0
92	152.5
93	29.0
94	18.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1081788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.59024316711283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.15146740844672	52.52967378407162
2	7.7337752463812235	9.217150950633101
3	1.6088342906306536	2.8761247978281044
4	0.6298370850399353	1.5012858021278108
5	0.3399405940905375	1.0128571332113967
6	0.22494749364446043	0.804280550766358
7	0.15486456625859274	0.6459892012923298
8	0.11646044095755187	0.5551924796807762
9	0.09643731270185296	0.5172050622857685
>10	0.7370111070871159	8.80091584730731
>50	0.10897403700321719	4.5368575034262895
>100	0.08750332633936497	10.0420121338529
>500	0.008082011777840728	3.2542879016712565
>1k	0.0013988097307801256	1.4214876260025173
>5k	4.662699102600419E-4	2.2846792258424613
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9507	0.8788228377463977	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8575	0.7926691736273651	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6586	0.6088069011673267	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4776	0.441491308833154	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1599	0.14781084648748186	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1389	0.12839854019456678	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1337	0.1235916833982259	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1306	0.12072605723117653	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.12063361767740075	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1291	0.11933946392453974	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1179	0.10898623390165171	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1166	0.10778451970256649	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.243955377578601E-5	0.0
2	0.0	0.0	0.0	4.6219776887893005E-4	0.0
3	0.0	0.0	0.0	5.546373226547161E-4	0.0
4	0.0	0.0	0.0	6.470768764305021E-4	0.0
5	0.0	0.0	0.0	9.243955377578601E-4	0.0
6	0.0	0.0	0.0	9.243955377578601E-4	0.0
7	0.0	0.0	0.0	9.243955377578601E-4	0.0
8	0.0	0.0	0.0	0.0011092746453094321	0.0
9	0.0	0.0	0.0	0.0019412306292915062	0.0
10	0.0	0.0	0.0	0.0040673403661345845	0.0
11	0.0	0.0	0.0	0.004252219473686157	0.0
12	0.0	0.0	0.0	0.005546373226547161	0.0
13	0.0	0.0	0.0	0.006101010549201877	0.0
14	0.0	0.0	0.0	0.007857362070941811	0.0
15	0.0	0.0	0.0	0.010353230022888033	0.0
16	0.0	0.0	0.0	0.015437405480556264	0.0
17	0.0	0.0	0.0	0.023849404874152793	0.0
18	0.0	0.0	0.0	0.0270847892563053	0.0
19	0.0	0.0	0.0	0.032446283375300894	0.0
20	0.0	0.0	0.0	0.03734557972541755	0.0
21	0.0	0.0	0.0	0.046774414210547725	9.243955377578601E-5
22	0.0	0.0	0.0	0.06433792942794707	9.243955377578601E-5
23	0.0	0.0	0.0	0.08883441117853036	9.243955377578601E-5
24	0.0	0.0	0.0	0.13126416636161614	1.8487910755157202E-4
25	0.0	0.0	0.0	0.14254179192226205	1.8487910755157202E-4
26	0.0	0.0	0.0	0.16139946089252238	1.8487910755157202E-4
27	0.0	0.0	0.0	0.18977840390168868	1.8487910755157202E-4
28	0.0	0.0	0.0	0.24607409215114237	1.8487910755157202E-4
29	0.0	0.0	0.0	0.3178996254349281	1.8487910755157202E-4
30	0.0	0.0	0.0	0.444079616338876	1.8487910755157202E-4
31	0.0	0.0	0.0	0.7742737024259837	1.8487910755157202E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCA	25	0.0054962034	29.599998	29
TTAACGG	40	5.939811E-5	27.750002	35
GCAGCGT	65	9.390715E-8	25.615385	1
GGACCGT	40	0.0019311139	23.125002	6
TCTAGCG	150	0.0	22.2	28
GCCGTCT	150	0.0	22.2	36
TAACGGC	50	2.7019606E-4	22.199999	36
CTAGCGG	155	0.0	21.483871	29
GACCGTG	55	5.1428244E-4	20.181818	7
CAGCGTC	85	1.2451692E-6	19.588236	2
AGCGTCA	90	2.1519772E-6	18.5	3
TTATGCG	60	9.236337E-4	18.5	4
CGGTCCA	215	0.0	18.069767	10
GCGGCGC	210	0.0	16.738096	32
CGCAATA	205	0.0	16.243904	36
GCGTTAT	160	6.2937033E-10	16.1875	1
ATGCCGT	220	0.0	15.977273	34
TGCCGTC	220	0.0	15.977273	35
CCGGTCC	255	0.0	15.960786	9
ACCGTGA	70	0.0025923622	15.857142	8
>>END_MODULE