FastQCFastQC Report
Fri 10 Feb 2017
ERR1630732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT852.2923408845738944TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT451.2135922330097086No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG411.1057173678532901No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC350.9439050701186623No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC270.7281553398058253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230.6202804746494067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160.4314994606256742No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC140.37756202804746497Illumina Paired End PCR Primer 2 (95% over 23bp)
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG130.35059331175836034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3236245954692557No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC120.3236245954692557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110.296655879180151No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG70.18878101402373249No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA60.16181229773462785No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA50.1348435814455232No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG50.1348435814455232No Hit
TCTGTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCC50.1348435814455232No Hit
GTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTAT50.1348435814455232No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG50.1348435814455232No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGCTGT50.1348435814455232No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC50.1348435814455232No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA50.1348435814455232No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG50.1348435814455232No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT50.1348435814455232No Hit
GCCTGAGGCAGGTGCTCGCTTGGTCTAGTGCCCATTTACTCTG50.1348435814455232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10787486515641855No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG40.10787486515641855No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC40.10787486515641855No Hit
AGCCACTGCTGGGTGCCCAGGGAGCCCCACTGGAGCCAGTGTA40.10787486515641855No Hit
CCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTATGCA40.10787486515641855No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG40.10787486515641855No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG40.10787486515641855No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG40.10787486515641855No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA40.10787486515641855No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC40.10787486515641855No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA40.10787486515641855No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG40.10787486515641855No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC40.10787486515641855No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)