FastQCFastQC Report
Fri 10 Feb 2017
ERR1630731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938564
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63980.681679672350527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52070.5547836908298209No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49920.5318763557945969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.43065789866221166No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.1263632527989567No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC11510.12263415174671093No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.11997050813796395No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA10280.10952902519167579No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.10761120179337796No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT9920.10569337839508015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA352.3878452E-531.71428515
CCGAATT555.1421276E-420.18181814
TAGACAG701.21889505E-418.55
AACATCG1451.546141E-1017.86206821
TACGACG904.4445587E-516.4444455
AGTCGGT1708.54925E-1116.32352811
TAGAACA803.3817376E-416.18754
CCCCGAA803.3817376E-416.187512
ATGGTCG803.3817376E-416.187536
AGTCGCC803.3817376E-416.187517
CCCGAAT803.3817376E-416.187513
TTAGACA1059.339368E-615.8571424
CAGTCCG700.002592018815.8571429
GCATCGC700.002592018815.85714214
TCGGGAT1302.587276E-715.6538454
TAACGAA957.0574686E-515.57894813
TACAGAG1802.0190782E-1015.4166665
CGAACGA855.36285E-415.23529316
TACGCTA855.36285E-415.23529326
TCGTATT855.36285E-415.23529326