##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630731.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	938564
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06230582038092	33.0	31.0	34.0	30.0	34.0
2	32.2279407690898	33.0	31.0	34.0	30.0	34.0
3	32.324313525769156	34.0	31.0	34.0	30.0	34.0
4	35.89701075259652	37.0	35.0	37.0	35.0	37.0
5	35.85867559377943	37.0	35.0	37.0	35.0	37.0
6	35.94182176175519	37.0	35.0	37.0	35.0	37.0
7	35.89125941331651	37.0	35.0	37.0	35.0	37.0
8	35.90664888063041	37.0	35.0	37.0	35.0	37.0
9	37.58588758997788	39.0	37.0	39.0	35.0	39.0
10	37.51413648936034	39.0	37.0	39.0	35.0	39.0
11	37.61012141953026	39.0	37.0	39.0	35.0	39.0
12	37.568600542957114	39.0	37.0	39.0	35.0	39.0
13	37.60814286505769	39.0	37.0	39.0	35.0	39.0
14	38.90132052795547	40.0	38.0	41.0	36.0	41.0
15	38.897434804659035	40.0	38.0	41.0	35.0	41.0
16	38.84933153199995	40.0	38.0	41.0	35.0	41.0
17	38.87026457439237	40.0	38.0	41.0	35.0	41.0
18	38.86831478727077	40.0	38.0	41.0	35.0	41.0
19	38.92139587710588	40.0	38.0	41.0	35.0	41.0
20	38.8946614189336	40.0	38.0	41.0	35.0	41.0
21	38.85298178920137	40.0	38.0	41.0	35.0	41.0
22	38.78048593383083	40.0	38.0	41.0	35.0	41.0
23	38.72844686137546	40.0	38.0	41.0	35.0	41.0
24	38.662234008549234	40.0	38.0	41.0	34.0	41.0
25	38.61139144480291	40.0	38.0	41.0	34.0	41.0
26	38.45432490485465	40.0	38.0	41.0	34.0	41.0
27	38.30872162154099	40.0	38.0	41.0	34.0	41.0
28	38.21000805485827	40.0	38.0	41.0	34.0	41.0
29	38.12864013535572	40.0	37.0	41.0	33.0	41.0
30	38.022904138662895	40.0	37.0	41.0	33.0	41.0
31	37.94532392037197	40.0	37.0	41.0	33.0	41.0
32	37.83274022868979	40.0	37.0	41.0	33.0	41.0
33	37.723715164868885	40.0	37.0	41.0	33.0	41.0
34	37.64286079585409	40.0	37.0	41.0	33.0	41.0
35	37.54933494146377	40.0	36.0	41.0	33.0	41.0
36	37.48718574332704	40.0	36.0	41.0	33.0	41.0
37	37.31360567846199	40.0	36.0	41.0	32.0	41.0
38	37.13750261037074	40.0	36.0	41.0	31.0	41.0
39	37.02777434463712	40.0	36.0	41.0	31.0	41.0
40	36.79555043662446	39.0	35.0	41.0	31.0	41.0
41	36.6856836614232	39.0	35.0	41.0	30.0	41.0
42	36.49411228216722	39.0	35.0	41.0	30.0	41.0
43	35.71303395399781	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	2.0
13	3.0
14	3.0
15	4.0
16	3.0
17	7.0
18	24.0
19	50.0
20	116.0
21	206.0
22	471.0
23	742.0
24	1313.0
25	2211.0
26	3365.0
27	5045.0
28	7256.0
29	10133.0
30	13637.0
31	17982.0
32	23003.0
33	29898.0
34	39037.0
35	52274.0
36	73534.0
37	116407.0
38	206423.0
39	335407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.66418912295806	19.65545237192136	12.064813907202918	23.61554459791767
2	18.877988075400292	21.65659454230079	33.70638549955038	25.75903188274854
3	21.82333863220835	24.362004082832925	28.514624468869464	25.300032816089256
4	14.75317612863907	15.53692662407678	33.9497359796455	35.760161267638644
5	14.176230922984473	37.87296337809675	33.41242579088906	14.538379908029714
6	34.51144514385807	33.70990150911392	15.864661333697008	15.913992013331004
7	28.324227223716232	31.115512634194364	21.846991787454026	18.713268354635378
8	28.572691899540146	31.536687961609438	20.293661380577138	19.596958758273278
9	27.142848010364766	13.768267267868787	20.976406510371163	38.11247821139528
10	18.39640131093884	27.24406646749716	31.886903823287486	22.472628398276516
11	34.06640356970862	22.673360580631687	21.372010859142264	21.88822499051743
12	22.428625005860017	26.697912981959675	28.579510827178538	22.293951185001767
13	30.06433232043846	20.44900507583926	26.038394824433926	23.448267779288358
14	22.94345404255863	21.662028375262636	26.46415161885604	28.93036596332269
15	25.876019110044705	27.419760399930105	23.964268819174826	22.739951670850363
16	24.260039805490088	26.28558095132564	25.919702865228157	23.534676377956114
17	23.038492846518725	26.833545714517072	26.480985846463323	23.646975592500887
18	22.360009546498695	25.00074582021045	28.858447585886527	23.78079704740433
19	23.65038505632008	25.543276750440036	28.99748978226312	21.80884841097677
20	24.09382844430428	24.68110858716081	28.612220370693954	22.612842597840956
21	24.200161097165456	25.0890722422765	28.311228642905544	22.3995380176525
22	24.235747375778317	25.28319858848198	28.32284212903968	22.158211906700025
23	23.543839311970203	25.42746152633171	28.00863872895189	23.020060432746195
24	22.885706249121	26.142276925175057	28.372812083139777	22.599204742564172
25	23.22793117997281	25.98778559586773	28.007360180019692	22.77692304413977
26	23.11328795905234	26.255428505674626	28.017588571477276	22.613694963795755
27	23.14919387489825	25.702456092498753	28.13010087751075	23.018249155092246
28	22.681244965713578	26.027100975532836	28.321776671596183	22.969877387157403
29	22.681244965713578	26.503893181498544	28.583879202696888	22.23098265009099
30	22.713208689018543	26.4999509889576	28.160572960394813	22.62626736162904
31	22.147770423753734	26.994003605507988	28.285124935539823	22.573101035198455
32	22.009154410354544	26.49334515280791	28.214378561291504	23.283121875546048
33	21.709121594265284	26.923470322748365	28.50940372739632	22.85800435559003
34	23.024428808264542	26.01388930323345	28.11422556160262	22.84745632689939
35	22.16875993539066	26.265017622666115	28.325931955625826	23.240290486317395
36	21.94043240524887	26.999117801236782	28.066279976645177	22.994169816869174
37	22.349674609296756	25.843096475040596	28.64013535571362	23.167093559949027
38	22.299704655196663	25.97766375015449	28.37675427568072	23.345877318968125
39	22.25080015854007	25.998653261791414	28.671246713063788	23.079299866604728
40	21.634326481731666	25.724297970090475	29.023593489628833	23.617782058549018
41	20.905766681867195	26.159537335759737	29.343443814167173	23.591252168205898
42	21.313091062516783	26.03242826275033	29.51317118491653	23.141309489816358
43	21.5143559735937	25.73228890091672	29.070473617142785	23.682881508346792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	405.0
1	362.5
2	320.0
3	734.0
4	1148.0
5	1148.0
6	1666.0
7	2184.0
8	2159.0
9	2134.0
10	3141.5
11	4149.0
12	4149.0
13	7186.0
14	10223.0
15	12926.5
16	15630.0
17	14964.5
18	14299.0
19	14299.0
20	16102.0
21	17905.0
22	15719.0
23	13533.0
24	14634.0
25	15735.0
26	15735.0
27	17561.0
28	19387.0
29	21521.5
30	23656.0
31	26265.5
32	28875.0
33	28875.0
34	32152.0
35	35429.0
36	38091.5
37	40754.0
38	43886.5
39	47019.0
40	47019.0
41	48588.0
42	50157.0
43	51729.5
44	53302.0
45	54506.0
46	55710.0
47	55710.0
48	58075.5
49	60441.0
50	59542.0
51	58643.0
52	57599.5
53	56556.0
54	56556.0
55	55064.5
56	53573.0
57	51866.5
58	50160.0
59	50516.0
60	50872.0
61	50872.0
62	47242.0
63	43612.0
64	39832.5
65	36053.0
66	31435.0
67	26817.0
68	26817.0
69	23753.0
70	20689.0
71	17300.0
72	13911.0
73	10551.5
74	7192.0
75	7192.0
76	5266.5
77	3341.0
78	2698.5
79	2056.0
80	1645.0
81	1234.0
82	1234.0
83	953.5
84	673.0
85	537.5
86	402.0
87	325.5
88	249.0
89	249.0
90	183.5
91	118.0
92	66.0
93	14.0
94	8.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	938564.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.50680670220111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.88875122776886	55.5615199099419
2	6.606948023410955	8.259584459816766
3	1.6413112125047091	3.0777936809456157
4	0.7085738012289401	1.7716274251064492
5	0.40324846717377283	1.2602886995294955
6	0.29706770616527045	1.1141252212043302
7	0.19909001772720336	0.8711136878088465
8	0.16288211792732793	0.8144992848422892
9	0.11978925949669424	0.6738879679523708
>10	0.7888696181117181	9.647284644168879
>50	0.10516023371135885	4.650457414082262
>100	0.07386286465768177	8.323515748268143
>500	0.0029066404601633413	1.18063066681654
>1k	0.0011968519541849052	1.5534051545377399
>5k	3.4195770119568717E-4	1.2402660349783605
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6398	0.681679672350527	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5207	0.5547836908298209	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4992	0.5318763557945969	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4042	0.43065789866221166	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1186	0.1263632527989567	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1151	0.12263415174671093	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1126	0.11997050813796395	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1028	0.10952902519167579	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1010	0.10761120179337796	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	992	0.10569337839508015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0654574434987918E-4	0.0
2	0.0	0.0	0.0	5.327287217493959E-4	0.0
3	0.0	0.0	0.0	0.0010654574434987919	0.0
4	0.0	0.0	0.0	0.0024505521200472212	0.0
5	0.0	0.0	0.0	0.0027701893530968588	0.0
6	0.0	0.0	0.0	0.002983280841796617	0.0
7	0.0	0.0	0.0	0.0038356467965956504	0.0
8	0.0	0.0	0.0	0.0039421925409455294	0.0
9	0.0	0.0	0.0	0.006818927638392268	0.0
10	0.0	0.0	0.0	0.009482571247139247	0.0
11	0.0	0.0	0.0	0.010974211668037555	0.0
12	0.0	0.0	0.0	0.012572397833285743	0.0
13	0.0	0.0	0.0	0.013637855276784535	0.0
14	0.0	0.0	0.0	0.015662224419432238	0.0
15	0.0	0.0	0.0	0.01939132547167801	0.0
16	1.0654574434987918E-4	0.0	0.0	0.02429242971177245	0.0
17	1.0654574434987918E-4	0.0	0.0	0.032070269049313635	0.0
18	2.1309148869975836E-4	0.0	0.0	0.03462736691371073	0.0
19	2.1309148869975836E-4	0.0	0.0	0.041233203063403244	0.0
20	2.1309148869975836E-4	0.0	0.0	0.045495032837398405	0.0
21	2.1309148869975836E-4	0.0	0.0	0.054764512595837894	0.0
22	2.1309148869975836E-4	0.0	0.0	0.06946782531612122	0.0
23	2.1309148869975836E-4	0.0	0.0	0.08928533376519875	0.0
24	3.1963723304963753E-4	0.0	0.0	0.12039669111536347	0.0
25	3.1963723304963753E-4	0.0	0.0	0.13051853682860198	0.0
26	3.1963723304963753E-4	0.0	0.0	0.14607421550368435	0.0
27	3.1963723304963753E-4	0.0	0.0	0.17164519414765536	0.0
28	4.2618297739951673E-4	0.0	0.0	0.2205496908042499	0.0
29	4.2618297739951673E-4	0.0	0.0	0.2895913331429716	0.0
30	5.327287217493959E-4	0.0	0.0	0.38910505836575876	0.0
31	5.327287217493959E-4	0.0	0.0	0.6650585362319458	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	35	2.3878452E-5	31.714285	15
CCGAATT	55	5.1421276E-4	20.181818	14
TAGACAG	70	1.21889505E-4	18.5	5
AACATCG	145	1.546141E-10	17.862068	21
TACGACG	90	4.4445587E-5	16.444445	5
AGTCGGT	170	8.54925E-11	16.323528	11
TAGAACA	80	3.3817376E-4	16.1875	4
CCCCGAA	80	3.3817376E-4	16.1875	12
ATGGTCG	80	3.3817376E-4	16.1875	36
AGTCGCC	80	3.3817376E-4	16.1875	17
CCCGAAT	80	3.3817376E-4	16.1875	13
TTAGACA	105	9.339368E-6	15.857142	4
CAGTCCG	70	0.0025920188	15.857142	9
GCATCGC	70	0.0025920188	15.857142	14
TCGGGAT	130	2.587276E-7	15.653845	4
TAACGAA	95	7.0574686E-5	15.578948	13
TACAGAG	180	2.0190782E-10	15.416666	5
CGAACGA	85	5.36285E-4	15.235293	16
TACGCTA	85	5.36285E-4	15.235293	26
TCGTATT	85	5.36285E-4	15.235293	26
>>END_MODULE