FastQCFastQC Report
Fri 10 Feb 2017
ERR1630730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729127
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA33620.19443337591744272No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT30870.17852939662615874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21050.12173773239328284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20150.11653279371613535No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20090.11618579780432554No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG19190.11098085912717806No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18210.10531325923428413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18020.10421443884688633No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA17640.1020167980720907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT200.001842192837.04
TACCGAT200.001842192837.07
TAGTCGT400.001931579223.12515
CTAGAAC7750.022.9161283
TAGAACT8600.022.1569774
GTCTAGA7400.022.01
CTAGACA7550.019.602654
TCTAGAC7000.019.2928583
GGTATCA13600.018.7720581
ACTGATC7600.018.7434228
ACAACGG7100.018.523
ACACCGT701.2196486E-418.56
TTATCGT500.007036282718.54
CGCCGTA500.007036282718.525
GTTCTAG7750.018.3806441
AGAACTG10850.017.9032275
ACGGAGT7250.017.86206826
GTGTACG1258.587449E-917.761
TCTAGAA9950.017.6633172
AGTACCG953.6085166E-617.5263165
Produced by FastQC (version 0.11.5)