##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630730.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1729127
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13397859150889	33.0	31.0	34.0	30.0	34.0
2	32.306956053546095	34.0	31.0	34.0	30.0	34.0
3	32.421826158518144	34.0	31.0	34.0	30.0	34.0
4	35.97363698560025	37.0	35.0	37.0	35.0	37.0
5	35.929445899578226	37.0	35.0	37.0	35.0	37.0
6	35.999727029882706	37.0	35.0	37.0	35.0	37.0
7	35.95252401934618	37.0	35.0	37.0	35.0	37.0
8	35.94303599446426	37.0	35.0	37.0	35.0	37.0
9	37.676760006639185	39.0	37.0	39.0	35.0	39.0
10	37.58957497049089	39.0	37.0	39.0	35.0	39.0
11	37.678006878615626	39.0	37.0	39.0	35.0	39.0
12	37.620004198650534	39.0	37.0	39.0	35.0	39.0
13	37.65300119655757	39.0	37.0	39.0	35.0	39.0
14	38.98114308549921	40.0	38.0	41.0	36.0	41.0
15	38.993420379185565	40.0	38.0	41.0	36.0	41.0
16	38.972530068641575	40.0	38.0	41.0	36.0	41.0
17	38.942970643567534	40.0	38.0	41.0	36.0	41.0
18	38.93115832440301	40.0	38.0	41.0	36.0	41.0
19	38.97465137031577	40.0	38.0	41.0	35.0	41.0
20	38.93283026637141	40.0	38.0	41.0	35.0	41.0
21	38.88741197147462	40.0	38.0	41.0	35.0	41.0
22	38.829183165840334	40.0	38.0	41.0	35.0	41.0
23	38.77875656328309	40.0	38.0	41.0	35.0	41.0
24	38.727779972205624	40.0	38.0	41.0	35.0	41.0
25	38.68634056376426	40.0	38.0	41.0	34.0	41.0
26	38.583209908815256	40.0	38.0	41.0	34.0	41.0
27	38.462686662113306	40.0	38.0	41.0	34.0	41.0
28	38.36878494176541	40.0	38.0	41.0	34.0	41.0
29	38.314004118841474	40.0	38.0	41.0	34.0	41.0
30	38.24647813607676	40.0	38.0	41.0	34.0	41.0
31	38.23086447669836	40.0	38.0	41.0	34.0	41.0
32	38.136416237789355	40.0	38.0	41.0	33.0	41.0
33	38.06017660935258	40.0	38.0	41.0	33.0	41.0
34	38.026720420188916	40.0	38.0	41.0	33.0	41.0
35	37.98911473824653	40.0	37.0	41.0	33.0	41.0
36	37.98279652101899	40.0	37.0	41.0	33.0	41.0
37	37.87426198307007	40.0	37.0	41.0	33.0	41.0
38	37.749992337173616	40.0	37.0	41.0	33.0	41.0
39	37.7145160534767	40.0	37.0	41.0	33.0	41.0
40	37.56409621733973	40.0	37.0	41.0	32.0	41.0
41	37.520647702568986	40.0	37.0	41.0	32.0	41.0
42	37.39638094830513	40.0	36.0	41.0	32.0	41.0
43	36.60150700324499	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	3.0
12	1.0
13	1.0
14	1.0
15	4.0
16	3.0
17	12.0
18	39.0
19	60.0
20	146.0
21	289.0
22	549.0
23	1087.0
24	1785.0
25	3078.0
26	4780.0
27	7269.0
28	10658.0
29	15157.0
30	21267.0
31	27891.0
32	36859.0
33	48631.0
34	65393.0
35	89723.0
36	128684.0
37	199339.0
38	385217.0
39	681192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94985041584568	18.19305348884148	10.957726066390727	25.899370028922107
2	19.60110506631381	20.644926601689754	33.326759688559605	26.427208643436835
3	20.687896262102207	21.84391314229666	28.508547955124175	28.959642640476957
4	14.502751966743913	14.348165288032632	33.75402732130144	37.39505542392202
5	15.336178314259161	36.088615815958	33.447225102609586	15.127980767173261
6	37.20189436634788	33.87634338021441	14.643632306938702	14.278129946499012
7	31.201756724636187	29.207860382724927	20.255597188639122	19.334785703999763
8	27.884707138342062	32.24361194984521	19.65812806115456	20.213552850658164
9	26.91288725466666	13.050631908471733	18.74217451928054	41.29430631758107
10	17.155767043137953	25.853740066519116	32.40768318347929	24.582809706863635
11	37.87229046796447	20.619942896039447	20.73786367340282	20.76990296259326
12	23.301758633113703	24.53966654849528	27.746197936877977	24.41237688151304
13	30.69190406488361	18.832046460439287	24.778688899080286	25.697360575596818
14	23.780612991411274	19.54413990412503	23.994940799605814	32.680306304857886
15	26.497475315578324	26.67606254485645	21.52247926265682	25.303982876908403
16	26.544204098368713	25.275702710095903	22.815501695364194	25.36459149617119
17	25.058078440739173	25.44648253135831	23.90258205441243	25.59285697349009
18	25.348745349531875	23.72989375563507	25.31080712984067	25.610553764992396
19	25.334229353887828	24.174453351315435	24.72172373689151	25.769593557905228
20	25.543757051969003	23.93942145371624	25.010193004909414	25.506628489405347
21	26.340054836920597	24.090653838613356	24.97584040964024	24.593450914825805
22	26.329529294262365	23.801721909379705	24.47113485591284	25.39761394044509
23	25.462849171865344	24.211177085315306	24.986192454342568	25.339781288476786
24	25.246208057592067	24.54117019744646	24.329271360634586	25.883350384326885
25	26.124454710382754	23.956655584002796	24.63763506092959	25.281254644684857
26	26.093398576275774	24.278031630990668	24.362004641648646	25.26656515108491
27	25.781391418906768	23.717170572202043	24.545160650432273	25.956277358458923
28	25.19913227888987	23.537484522536516	25.6649164578426	25.59846674073102
29	24.919048745407366	24.207591460893273	24.9448999408372	25.92845985286217
30	24.252874427384455	25.119034055913765	25.55040780694535	25.07768370975643
31	25.939043228172366	24.5837928619471	24.2673904230285	25.20977348685204
32	25.296869460716305	24.356510539711657	24.269530231151325	26.077089768420713
33	25.001344609158267	24.083019928553544	25.223074996804744	25.69256046548345
34	26.09657937213403	23.607866859981943	24.738842201874125	25.5567115660099
35	25.123371504811388	24.231418513504213	25.66173566198434	24.983474319700058
36	25.211913294974863	24.03334168051277	25.321679668410706	25.433065356101665
37	25.56156950877524	24.111126597410138	25.165531508096283	25.16177238571834
38	24.791354249861346	23.783735954617562	25.83066483838376	25.59424495713733
39	25.47742300016135	23.80941365209149	25.534388162350137	25.178775185397022
40	24.76770069520631	24.29983454078272	25.915158342909457	25.017306421101516
41	25.05374099184155	23.9062833441384	25.96790172150455	25.072073942515498
42	25.274950885620317	23.856605096097628	26.07228965830734	24.796154359974715
43	24.200420212049202	23.355832162704072	26.036664744694864	26.40708288055186
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	72.0
2	81.0
3	259.0
4	437.0
5	437.0
6	570.5
7	704.0
8	684.0
9	664.0
10	957.5
11	1251.0
12	1251.0
13	2078.0
14	2905.0
15	3953.5
16	5002.0
17	5178.5
18	5355.0
19	5355.0
20	6390.5
21	7426.0
22	7629.0
23	7832.0
24	9563.5
25	11295.0
26	11295.0
27	14073.5
28	16852.0
29	20798.5
30	24745.0
31	29245.5
32	33746.0
33	33746.0
34	41524.5
35	49303.0
36	56786.0
37	64269.0
38	71151.5
39	78034.0
40	78034.0
41	85215.0
42	92396.0
43	99079.0
44	105762.0
45	114711.5
46	123661.0
47	123661.0
48	127480.5
49	131300.0
50	133519.5
51	135739.0
52	138408.0
53	141077.0
54	141077.0
55	134571.0
56	128065.0
57	123352.5
58	118640.0
59	114225.0
60	109810.0
61	109810.0
62	102663.0
63	95516.0
64	87387.0
65	79258.0
66	70484.5
67	61711.0
68	61711.0
69	49928.0
70	38145.0
71	32331.0
72	26517.0
73	19895.5
74	13274.0
75	13274.0
76	10261.0
77	7248.0
78	5804.0
79	4360.0
80	3659.5
81	2959.0
82	2959.0
83	2222.5
84	1486.0
85	1244.0
86	1002.0
87	860.5
88	719.0
89	719.0
90	569.0
91	419.0
92	239.5
93	60.0
94	40.5
95	21.0
96	21.0
97	19.5
98	18.0
99	9.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1729127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.450559084208315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.83743534950062	41.458080502189745
2	8.79386269319928	8.697228533769326
3	2.6009440915437123	3.8585441842081467
4	1.261434914592129	2.495146470996854
5	0.7099597493733377	1.755395326689798
6	0.46985377084014884	1.3940718993521313
7	0.3307764675770956	1.1449956877510818
8	0.2505417812486662	0.9911544925359969
9	0.18668618565893524	0.8308562628719395
>10	1.2635754857676609	12.195941587137812
>50	0.1481868161933966	5.145887703494643
>100	0.1297207425033903	12.803500661205893
>500	0.013147990511623981	4.367960157550213
>1k	0.0038739614900320666	2.861236530246467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	3362	0.19443337591744272	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	3087	0.17852939662615874	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2105	0.12173773239328284	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2015	0.11653279371613535	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2009	0.11618579780432554	No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG	1919	0.11098085912717806	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1821	0.10531325923428413	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1802	0.10421443884688633	No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA	1764	0.1020167980720907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.783265196830539E-5	0.0
2	0.0	0.0	0.0	9.831550834611916E-4	0.0
3	0.0	0.0	0.0	0.0012144856913344133	0.0
4	0.0	0.0	0.0	0.002197640774795605	0.0
5	0.0	0.0	0.0	0.002313306078732216	0.0
6	0.0	0.0	0.0	0.002371138730700521	0.0
7	0.0	0.0	0.0	0.0025446366866054373	0.0
8	0.0	0.0	0.0	0.0027181346425103536	0.0
9	0.0	0.0	0.0	0.004742277461401042	0.0
10	0.0	0.0	0.0	0.007518244755879701	0.0
11	0.0	0.0	0.0	0.008385734535404282	0.0
12	0.0	0.0	0.0	0.009253224314928863	0.0
13	0.0	0.0	0.0	0.009947216138548527	0.0
14	0.0	0.0	0.0	0.010699040614136499	0.0
15	0.0	0.0	0.0	0.0117978610015343	0.0
16	0.0	0.0	0.0	0.013359342604678547	0.0
17	0.0	0.0	0.0	0.01532565277160093	0.0
18	0.0	0.0	0.0	0.016366640507030426	5.783265196830539E-5
19	0.0	0.0	0.0	0.01781245680623806	5.783265196830539E-5
20	0.0	0.0	0.0	0.019547436365287222	5.783265196830539E-5
21	0.0	0.0	0.0	0.021802909792051132	5.783265196830539E-5
22	0.0	0.0	0.0	0.0246367097384981	5.783265196830539E-5
23	0.0	1.7349795590491617E-4	0.0	0.029957313719582195	5.783265196830539E-5
24	0.0	2.89163259841527E-4	0.0	0.035104419744761375	5.783265196830539E-5
25	0.0	2.89163259841527E-4	0.0	0.037186395215620366	5.783265196830539E-5
26	0.0	2.89163259841527E-4	0.0	0.0407720196376553	5.783265196830539E-5
27	0.0	2.89163259841527E-4	0.0	0.04661311748645415	5.783265196830539E-5
28	0.0	4.0482856377813776E-4	0.0	0.06072428456672066	5.783265196830539E-5
29	0.0	5.204938677147485E-4	0.0	0.08316335353042316	5.783265196830539E-5
30	0.0	5.204938677147485E-4	0.0	0.11740028349565995	5.783265196830539E-5
31	0.0	5.204938677147485E-4	0.0	0.24307063622278757	5.783265196830539E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACT	20	0.0018421928	37.0	4
TACCGAT	20	0.0018421928	37.0	7
TAGTCGT	40	0.0019315792	23.125	15
CTAGAAC	775	0.0	22.916128	3
TAGAACT	860	0.0	22.156977	4
GTCTAGA	740	0.0	22.0	1
CTAGACA	755	0.0	19.60265	4
TCTAGAC	700	0.0	19.292858	3
GGTATCA	1360	0.0	18.772058	1
ACTGATC	760	0.0	18.743422	8
ACAACGG	710	0.0	18.5	23
ACACCGT	70	1.2196486E-4	18.5	6
TTATCGT	50	0.0070362827	18.5	4
CGCCGTA	50	0.0070362827	18.5	25
GTTCTAG	775	0.0	18.380644	1
AGAACTG	1085	0.0	17.903227	5
ACGGAGT	725	0.0	17.862068	26
GTGTACG	125	8.587449E-9	17.76	1
TCTAGAA	995	0.0	17.663317	2
AGTACCG	95	3.6085166E-6	17.526316	5
>>END_MODULE