FastQCFastQC Report
Fri 10 Feb 2017
ERR1630729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028499
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103601.0072931524483737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99600.9684015249407145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74690.7262039146367668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52220.5077301971124911No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.13874588113357425No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.12873128705035203No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.11988344179235955No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA11920.11589704997282448No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA11540.11220234535959685No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11200.10889655702144582No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC10360.10072931524483739No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA450.003825538320.5555552
AGCGTAT555.1425875E-420.18181814
CGTAGAC609.2359167E-418.53
AACGCTA701.2190391E-418.533
TTGCGCG902.151768E-618.518
GCAGTCG1156.4030246E-817.6956529
ATTAGGC852.7226644E-517.4117643
ACGCTAA752.067E-417.26666634
GTATTGG650.001579781417.0769231
TAACGCT650.001579781417.07692332
ATACCGT1107.805629E-716.8181826
CAGTCGG1005.87658E-616.65000210
TCTATAC803.3821326E-416.18753
GGTATCA47950.015.9343071
CAATACG1403.4713594E-815.8571424
CTCTATG2700.015.7592581
AAGACGG1659.731593E-1015.696975
TCGGTGA1201.9349209E-615.41666613
ATAAGAC1455.3427357E-815.3103453
CTTAACG855.363469E-415.23529430
Produced by FastQC (version 0.11.5)